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Dmitriy Khodakov Leigh Thredgold Claire E. Lenehan Gunther G. Andersson Hilton Kobus Amanda V. Ellis 《Biomicrofluidics》2012,6(2)
Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional “tag sequence” linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes. 相似文献
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ABSTRACTBelonging, Being and Becoming: The Early Years Learning Framework for Australia emphasises that families have an important role in their children's learning and it recognises that their earliest development is influenced through these relationships and adds that partnerships can be fostered with families by early childhood educators sharing the children's documented experiences. The research discussed employed an in-depth structured questionnaire. It involved 37 families with children aged from three to five years who attended an early learning centre in Northern Tasmania, Australia. The aim of this research was to determine these families’ perceptions, beliefs and experiences of educators sharing children's learning through pedagogical documentation. The documentation included hard copy and digital formats of the children's learning, capturing their voices and explorations. The findings show that families consider the sharing of documentation fosters family conversations about the children's learning experiences and helps to create stronger connections between the centre, home and extended family. Another recurring theme from the families’ responses was that children gain pride and a positive sense of identity when their documented work is shared with families. 相似文献
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Pupil involvement in special educational needs disagreement resolution: some perceived barriers to including children in mediation 总被引：1，自引：0，他引：1
Kirstie Soar Katie Burke Katia Herbst Irvine Gersch 《British Journal of Special Education》2005,32(1):35-41
Recent legislation in England has encouraged the use of disagreement resolution and mediation and emphasised the need to involve pupils in their own schooling. These policies apply in the educational system generally, but are particularly significant in the area of special educational needs (SEN). Kirstie Soar, a lecturer in the School of Psychology at the University of East London; Katie Burke, a PhD student at Salford University, Manchester; Katia Herbst, an independent researcher offering research and development services to the not-for- profit sector; and Professor Irvine Gersch, Director of the MSc educational psychology programme at the University of East London, set out to examine how pupil involvement in informal disagreement resolution has developed across 11 English regions since its introduction. The research consisted of 12 in-depth interviews with local education authority (LEA) SEN officers, mediators and parent partnership officers (PPOs) involved in informal special educational needs disagreement resolution. The aims of the study were to determine how far children were actually involved in mediation and what, if any, barriers existed which were seen to restrict such pupil involvement. A thematic analysis of interview content was conducted. Four major themes emerged, including: the distinction between direct and indirect pupil involvement; the importance of the child's view and how it is elicited; the role of other agencies; and other barriers to pupil involvement in informal disagreement resolution. Direct pupil involvement was found to be limited and variable, but indirect pupil involvement was more prevalent. In this article, the authors note a series of recommendations concerning pupil involvement in the mediation process and, in conclusion, put forward the implications of their work for future policy, practice and research. 相似文献
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This case study research reports on elementary (grade 8) and secondary school (grade 9) teachers’ participation in job-embedded, professional learning and engagement in collaborative inquiry. Teachers constructed an inquiry-oriented media literacy unit following the collaborative inquiry model. The current study sought to investigate how collaborative inquiry influenced their self-efficacy in literacy instruction, instructional and assessment practices. Qualitative data sources included researchers’ anecdotal notes, teacher interviews, and professional learning blog conversations. Inductive analyses of the data generated six major themes comprising enhancements to teachers’ literacy instructional and assessment practices, which were evident in their adoption of the backward-design model, use of success criteria, and greater emphasis on diagnostic and formative assessment. With respect to teachers’ self-efficacy, data analyses also revealed increased levels of intrinsic motivation, professional satisfaction, and reflective practice. This study provides evidence for the transformative potential and characteristics of school-based teacher collaborative inquiry and the resultant impacts on teacher learning. 相似文献
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In this single-case study, we examined the perceived influence of school-wide Apple iPod Touch integration on student learning and engagement. Data collection consisted of elementary teacher and principal interviews, as well as parent surveys. Findings revealed that there was a marked shift in teachers’ and students’ perceived roles when the iPod Touch was used. Consistent with current pedagogical practices, the findings also suggested that mobile technology facilitated self-directed learning, peer mentoring, differentiated instruction, and formative assessment, and enhanced student engagement. Caveats are offered regarding the integration of these mobile devices into K–8 schools and the use of the iPod Touch as a way to extend the support of learning beyond the classroom. 相似文献
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Katia Lehraus 《Education 3-13》2015,43(1):55-69
This study explored how to integrate cooperative skills training into learning tasks in the area of writing. Cooperative learning sessions, aimed at developing both cooperative and cognitive skills, were created and conducted in two elementary school classes (Grade 2, age 7–8). Pupils’ teamwork interactions were videotaped and analysed. Results show that young pupils were able to work cooperatively on writing tasks (WT) without teacher's help, advocating realisable teaching practices. Interactive dynamics likely to enhance pupils’ involvement in constructive interactions and in WT are documented; this typology could be used as a heuristic tool in future qualitative research. 相似文献
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Katia Bernardeschi Cristina Padovani Giuseppe Pasquinelli 《Journal of Cultural Heritage》2004,5(4):92
The paper describes the numerical techniques implemented in the finite-element code NOSA for structural analysis of masonry constructions. The code is then applied to the analysis of “Buti’s bell tower”, a medieval structure located on the Pisa mountains, under two different conditions: firstly, with the structure subjected to its own weight alone, and then while subjected to both its own weight and a horizontal load, which models an earthquake. The displacement and stress fields, as well as the distribution of cracking have been calculated with NOSA, and the numerical results analysed and compared to the actual distribution of fractures in the tower. 相似文献
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The influence of the sleep patterns of 2,259 students, aged 11 to 14 years, on trajectories of depressive symptoms, self-esteem, and grades was longitudinally examined using latent growth cross-domain models. Consistent with previous research, sleep decreased over time. Students who obtained less sleep in sixth grade exhibited lower initial self-esteem and grades and higher initial levels of depressive symptoms. Similarly, students who obtained less sleep over time reported heightened levels of depressive symptoms and decreased self-esteem. Sex of the student played a strong role as a predictor of hours of sleep, self-esteem, and grades. This study underscores the role of sleep in predicting adolescents' psychosocial outcomes and highlights the importance of using idiographic methodologies in the study of developmental processes. 相似文献
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This study analyzed the effect of selected variables on attrition in the Introductory Course of the National Open University of Venezuela. The findings suggested that noninstitutional variables accounted for the largest proportion of variance of student completion. Counseling and course redesign may enhance students’ motivation to persist successfully. 相似文献