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After Stuart Hall’s death in 2014, Korean newspapers ran detailed obituaries praising him as an influential British intellectual figure. The broad media attention appears to be unusual, given that he maintained a relatively unknown presence in the Korean cultural fields compared with other theorists. This work examines how and in which context Hall’s writings have been cited, or not cited, and emphasized in the Korean cultural studies. The incorporation of Hall’s writings into the resources of the academic field entangled him in scholarly rituals of parochial citations and applications, resulting in the fragmentation and de-contextualization of Hall’s overall problem. The selective focus on and occasional absence of certain aspects among his intellectual and political legacies may demonstrate how imported British cultural studies have, or not yet, been indigenized and localized in Korean cultural and political contexts.  相似文献   
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We present an engineered three-dimensional (3D) in vitro brain microvasculature system embedded within the bulk of a collagen matrix. To create a hydrogel template for the functional brain microvascular structure, we fabricated an array of microchannels made of collagen I using microneedles and a 3D printed frame. By culturing mouse brain endothelial cells (bEnd.3) on the luminal surface of cylindrical collagen microchannels, we reconstructed an array of brain microvasculature in vitro with circular cross-sections. We characterized the barrier function of our brain microvasculature by measuring transendothelial permeability of 40 kDa fluorescein isothiocyanate-dextran (Stoke''s radius of ∼4.5 nm), based on an analytical model. The transendothelial permeability decreased significantly over 3 weeks of culture. We also present the disruption of the barrier function with a hyperosmotic mannitol as well as a subsequent recovery over 4 days. Our brain microvasculature model in vitro, consisting of system-in-hydrogel combined with the widely emerging 3D printing technique, can serve as a useful tool not only for fundamental studies associated with blood-brain barrier in physiological and pathological settings but also for pharmaceutical applications.  相似文献   
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Three-dimensional (3D) printing technology has become more affordable, accessible, and relevant in healthcare, however, the knowledge of transforming medical images to physical prints still requires some level of training. Anatomy educators can play a pivotal role in introducing learners to 3D printing due to the spatial context inherent to learning anatomy. To bridge this knowledge gap and decrease the intimidation associated with learning 3D printing technology, an elective was developed through a collaboration between the Department of Anatomy and the Makers Lab at the University of California, San Francisco. A self-directed digital resource was created for the elective to guide learners through the 3D printing workflow, which begins with a patient's computed tomography digital imaging and communication in medicine (DICOM) file to a physical 3D printed model. In addition to practicing the 3D printing workflow during the elective, a series of guest speakers presented on 3D printing applications they utilize in their clinical practice and/or research laboratories. Student evaluations indicated that their intimidation associated with 3D printing decreased, the clinical and research topics were directly applicable to their intended careers, and they enjoyed the autonomy associated with the elective format. The elective and the associated digital resource provided students with the foundational knowledge of 3D printing, including the ability to extract, edit, manipulate, and 3D print from DICOM files, making 3D printing more accessible. The aim of disseminating this work is to help other anatomy educators adopt this curriculum at their institution.  相似文献   
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The Internet of Things (IoT) is gaining importance in education owing to its rapid development. This study addresses the importance of interdisciplinary education between technology and the humanities. The use of films as a teaching resource is suitable for interdisciplinary education because films represent creative forecasts and predictions on future human society, helping the public realize what could occur in the future. By utilizing films in instruction, students can be trained to understand the three key functions of IoT and acquire the ability to develop creative applications of IoT. An IoT teaching model using the films Minority Report (2002), Big Hero 6 (2014), Iron Man 3 (2013), Her (2013), and Transcendence (2014), all of which depict various applications of IoT that could be helpful in the teaching of the functions and concepts of IoT, is suggested; an interdisciplinary class is carried out for 15 weeks following the model. The effectiveness of the class is measured using quantitative analysis, qualitative analysis, and reflection journals. The findings show that students developed interdisciplinary capacity through education using films.  相似文献   
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A simple microfluidic 3D hydrodynamic flow focusing device has been developed and demonstrated quantitative determinations of quantum dot 525 with antibody (QD525-antibody) and hemagglutinin epitope tagged MAX (HA-MAX) protein concentrations. This device had a step depth cross junction structure at a hydrodynamic flow focusing point at which the analyte stream was flowed into a main detection channel and pinched not only horizontally but also vertically by two sheath streams. As a result, a triangular cross-sectional flow profile of the analyte stream was formed and the laser was focused on the top of the triangular shaped analyte stream. Since the detection volume was smaller than the radius of laser spot, a photon burst histogram showed Gaussian distribution, which was necessary for the quantitative analysis of protein concentration. By using this approach, a linear concentration curve of QD525-antibody down to 10 pM was demonstrated. In addition, the concentration of HA-MAX protein in HEK293 cell lysate was determined as 0.283 ± 0.015 nM. This approach requires for only 1 min determining protein concentration. As the best of our knowledge, this is the first time to determinate protein concentration by using single molecule detection techniques.  相似文献   
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ABSTRACT

Employing the multiple-baseline across-subjects design, the authors examined the implementation and potential effect of a virtual-reality-based social interaction program on the interaction and communication performance of children with high functioning autism. The data were collected via behavior observation and analysis, questionnaires, and interviewing. The children participants demonstrated increased performance of responding, initiation, greeting, and positive conversation-ending during the intervention, and improved social competence measures after the intervention. The study also contributed salient themes on the adaptive design of a virtual-reality-based learning environment for learners with special needs. The study findings should extend the discussion on the design and usage of technology-supported informal learning environment for children with diverse characteristics and learning needs.  相似文献   
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This article describes collaboration between preschool and university educators focused on adapting the Center for Research on Education, Diversity, and Excellence (CREDE) standards for Effective Pedagogy for use in early childhood (EC) settings. The CREDE standards are strategies of best practices for culturally diverse K–12 students. Teachers and teacher educators worked together to adapt the CREDE standards for use with young children. Participants included 13 preschool teachers and 2 administrators serving 2- to 5-years-olds at a university-based EC center. Data sources included participant observations and video-recordings of the teachers' instruction. Criteria for measuring use of the standards were changed to be more developmentally appropriate, including adjustments for language development and a focus on goals that included self-management and social skills. The adapted CREDE EC standards may provide a useful framework for high-quality EC instruction for diverse children. This partnership may have been successful because the project drew on the strengths of both the university and EC collaborators, minimizing the hierarchical relationship that is often experienced in such an arrangement.  相似文献   
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Im do J  Noh J  Yi NW  Park J  Kang IS 《Biomicrofluidics》2011,5(4):44112-4411210
We experimentally investigate the effects of high electric field on living cells inside a charged droplet under electrophoretic actuation. When an aqueous droplet suspended in a dielectric liquid contacts with electrified electrode, the droplet acquires charge. This charged droplet undergoes electrophoretic motion under strong electric field (1–3 kV/cm), which can be used as a droplet manipulation method in biomicrofluidic applications. However, because strong electric field and use of dielectric oil can be a harmful environment for living cells, the biological feasibilities have been tested. Trypan blue test and cell growth test have been performed to check the viability and proliferation of cells in a droplet under various electric field strengths and actuation times. We have not observed any noticeable influence of electric field and silicone oil on the viability and proliferation of cells, which indicates that electrophoresis could be safely used as a manipulation method for a droplet containing living biological system.  相似文献   
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