Effective cell collection method using collagenase and ultrasonic vibration     

Y. Kurashina  K. Takemura  S. Miyata  J. Komotori  T. Koyama 《Biomicrofluidics》2014,8(5)

This study proposes a novel cell collection method based on collagenase treatment and ultrasonic vibration. The method collects calf chondrocytes from a reusable metal cell culture substrate. To develop our concept, we calculated the natural vibration modes of the cell culture substrate by a finite element method, and conducted eigenvalue and piezoelectric-structural analyses. Selecting the first out-of-plane vibration mode of the substrate, which has a single nodal circle, we designed and fabricated the cell collection device. The excited vibration mode properly realized our intentions. We then evaluated the cell collection ratio and the growth response, and observed the morphology of the collected cells. The collagenase and ultrasonic vibration treatment collected comparable numbers of cells to conventional trypsin and pipetting treatment, but improved the proliferating cell statistics. Morphological observations revealed that the membranes of cells collected by the proposed method remain intact; consequently, the cells are larger and rougher than cells collected by the conventional method. Therefore, we present a promising cell collection method for adhesive cell culturing process.  相似文献   

Learning to Teach Science: The Curriculum of Student Teaching in Hiroshima "Attached" Schools     

Tippins  Deborah J.  Kemp  Andy  Ogura  Yasushi  Akiyama  Mikio  Ikeda  Hideo  Isozaki  Tetsuo  Ochiai  Hiroshi  Ohshika  Kiyoyuki  Takemura  Shigekazu  Bryan  Lynn  Keys  Carolyn  Padilla  Michael  Riley  Joseph P.  Thomson  Norman 《Journal of Science Teacher Education》2000,11(3):189-206

Journal of Science Teacher Education -  相似文献   

Effects of Reward on Self‐regulation,Intrinsic Motivation and Creativity     

Marcus Selart  Thomas Nordström  Bård Kuvaas  Kazuhisa Takemura 《Scandinavian Journal of Educational Research》2013,57(5):439-458

This article evaluates the effects of two types of rewards (performance‐contingent versus engagement‐contingent) on self‐regulation, intrinsic motivation and creativity. Forty‐two undergraduate students were randomly assigned to three conditions; i.e. a performance‐contingent reward group, an engagement‐contingent reward group and a control group. Results provide little support for the negative effects of performance rewards on motivational components. However, they do indicate that participants in the engagement‐contingent reward group and the control group achieved higher rated creativity than participants in the performance‐contingent reward group. Alternative explanations for this finding are discussed.  相似文献