排序方式： 共有71条查询结果，搜索用时 31 毫秒
1.
Christopher R. Phaneuf Nikita Pak D. Curtis Saunders Gregory L. Holst Joav Birjiniuk Nikita Nagpal Stephen Culpepper Emily Popler Andi L. Shane Robert Jerris Craig R. Forest 《Biomicrofluidics》2015,9(4)
Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously—each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel. 相似文献
2.
A number of recent studies have measured the extent and timing of segment rotation during the golf swing. A promising technique,
instantaneous screw axis (ISA) theory, could provide a better expression of segment rotation. In Part 1 of this two-part study,
the objectives are to identify the ISA of the pelvis, shoulders and left arm during the downswing, compute segment angular
velocity relative to that segment’s ISA and verify that ISA theory is a valid tool to analyse segment rotation during the
golf swing. Results indicate that for all subjects, at least 71% of marker velocity is a result of rotation about their respective
ISA, when averaging results over the duration of the downswing, confirming that motion is primarily rotational. Furthermore,
ISA position and orientation of each segment approaches, on average, the expected gross axis of rotation, confirming that
motion about the ISA is representative of joint motion. 相似文献
3.
低碳理念促进体育产业可持续发展的策略分析 总被引：1，自引：0，他引：1
季锋 《体育科技文献通报》2011,19(10):83-83,89
低碳理念与低碳经济发展模式,作为减少二氧化碳排放、缓解全球变暖的重要理论与实践支持,成为企业绿色环保发展的重要指标。体育产业通过落实低碳理念,能够更好地促进经济的发展,提高体育产业升级的速度。本文在分析低碳理念对促进体育产业发展意义的基础上,对我国体育产业的发展现状进行了研究,最后提出了低碳经济理念下我国体育产业发展的策略。 相似文献
4.
Forest B. Wortham 《About Campus》2013,18(1):21-24
With the effectiveness of traditional methods of connecting with incoming Wittenberg University minority students waning, Forest B. Wortham spans the virtual and physical worlds to help them find their place. 相似文献
5.
目前制约森林公安派出所顺利开展工作的一些问题主要表现在派出所隶属关系不规范,派出所机构摊子大、规模小、人员少,职能作用没有得到充分发挥。加强和改革森林公安派出所工作要从理顺隶属关系,充实派出所警力等方面入手, 不断强化派出所的职能作用。 相似文献
6.
ABSTRACTThis paper presents an account of a cross-sectorial study investigating whether shared understandings of practices might enhance continuity during transitions to school. Applying an ontological lens to cross-sectorial leading practices, the paper sheds light on the contextualised realities of transitions to school as a site specific lived experience. The paper begins with an overview of the ways transitions to school practices are understood, including emerging perspectives of transitions as continuity practices. Then, cross-sectorial concepts of leadership and leading are presented to highlight fundamental differences in the ways these practices are enacted across the sectors. The paper presents empirical evidence of the ways leading practices can work to establish shared understandings of transitions to school practices and policies. Given ‘little research has been done on the direct effects of leadership on transitions’ (OECD. [2017]. Starting Strong V: Transitions from Early Childhood Education and Care to Primary School. Paris: OECD Publications, 95) the paper contributes to emerging discourses that construct transitions to school as continuities. 相似文献
7.
高庸 《重庆职业技术学院学报》2005,14(1):144-145
巨额财产来源不明罪，从其犯罪主体上看具有其特殊性，其犯罪侵犯了国家工作人员廉洁自律制度，危害了国家正常的秩序，具有很大的危害性，《刑法》中加大对此类犯罪的打击力度。 相似文献
8.
将落叶松，樟子松种子放在元极堂内三元场处理和发放三元能量处理，播种后出苗数分别比对照组多４０．７％，１２８．１％和６１．６％、１３６．２％，保苗株数分别比对照组多１１４．１％，１１８．４％和１６５．３％，２０２．０％，防病效果分别比对照组提高７７．７％，６８．０％和７７．１％，６５．６％，表明可将三元能量应用于造林。 相似文献
9.
信息素养和终身学习领域的图书馆员和其他信息专业人员的职业和专业发展机会与挑战 总被引：3，自引：0，他引：3
在今天和明天的培训班上,大家会反复地听到"信息素养和终身学习".我很荣幸作为联合国教科文组织信息素养项目的推广者,今年已在全世界所有的地区主办了11个培训班,其中6个已经完成.在这6个培训班上,专家和学员充分强调和讨论了该项目带来的职业和专业发展机会与挑战.今天,我愿意与大家分享这些成果. 相似文献
10.
ObjectiveThe present study extends field research on interviews with young children suspected of having been abused by examining multiple assessment interviews designed to be inquisitory and exploratory, rather than formal evidential or forensic interviews.MethodsSixty-six interviews with 24 children between the ages of 3 and 6 years who were undergoing an assessment for suspected child abuse were examined. Each child was interviewed 2, 3, or 4 times. The interviewer's questions were categorized in terms of openness (open, closed or choice), in terms of the degree of interviewer input (free recall, direct, leading, suggestive), and for topic (whether the question was abuse-specific or nonabuse-related). Children's on-task responses were coded for amount of information (number of clauses) reported in relation to each question type and topic, and off-task responses were categorized as either ignoring the question or a diverted response.ResultsChildren provided a response to most questions, independent of question type or topic and typically responded with one or two simple clauses. Some children disclosed abuse in response to open-ended questions; generally, however, failure to respond to a question was more likely for abuse-specific than for nonabuse-related questions.ConclusionThe findings are discussed in terms of the growing literature on interviewing children about suspected abuse, particularly in interviews conducted over multiple sessions.Practice implicationsAssessment of suspected child abuse may involve more than a single investigative interview. Research examining children's responses to questioning over multiple interviews (or single interviews conducted over multiple sessions) is necessary for the development of best practise guidelines for the assessment of abuse. 相似文献