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1.
The aim of this study was to investigate the effect of low-intensity exercise training using belt electrode skeletal muscle electrical stimulation on muscle strength and cardiorespiratory fitness in healthy subjects. Nineteen healthy subjects were allocated into control or intervention groups; in both groups the participants kept regular physical activity while the intervention group underwent 30 min B-SES training at 3–4 METs for four weeks. Knee extensor muscle strength and cardiorespiratory endurance during incremental exercise test were measured at baseline and after four weeks for all participants. The relative change of knee extensor muscle strength in the intervention group was significantly higher than control group (p?p?相似文献   
2.
Purpose: Brain-derived neurotrophic factor (BDNF) is well known for its potential to promote brain plasticity. It has been proposed that combining cognitive and physical exercise (CCPE) may have the potential to generate more synergistic benefits in cognitive function than either cognitive exercise (CE) or physical exercise (PE) alone. The purpose of this study was to examine acute responses of peripheral BDNF levels and cognitive performance to CE, PE, and CCPE.

Methods: Thirteen healthy adult men participated in four experimental sessions; a 30-min CE, a 30-min cycling PE at an intensity of 60% peak oxygen uptake, a 30-min CCPE at the same intensity as PE, and a 30-min session of complete rest. Plasma BDNF levels and cognitive performance were measured before and after each session.

Results: Both PE and CCPE significantly increased plasma BDNF levels (p?p?≥?.05), and there was no significant difference in peripheral BDNF levels between PE and CCPE (p?≥?.05). No session induced a significant change in cognitive performance (p?≥?.05).

Conclusions: Our study suggests that CE and PE have different responses of peripheral BDNF levels and that CCPE had no additional or synergistic effect on peripheral BDNF levels compared with PE alone. This study offers further insights into the potential mechanisms underlying the respective roles of CE, PE, and CCPE for peripheral BDNF levels and cognitive performance.  相似文献   
3.
A microfluidic device was successfully fabricated for the rapid serodiagnosis of amebiasis. A micro bead-based immunoassay was fabricated within integrated microfluidic chip to detect the antibody to Entamoeba histolytica in serum samples. In this assay, a recombinant fragment of C terminus of intermediate subunit of galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica (C-Igl, aa 603-1088) has been utilized instead of the crude antigen. This device was validated with serum samples from patients with amebiasis and showed great sensitivity. The serodiagnosis can be completed within 20 min with 2 μl sample consumption. The device can be applied for the rapid and cheap diagnosis of other infectious disease, especially for the developing countries with very limited medical facilities.Entamoeba histolytica is the causative agent of amebiasis and is globally considered a leading parasitic cause of human mortality.1 It has been estimated that 50 × 106 people develop invasive disease such as amebic dysentery and amebic liver abscess, resulting in 100 000 deaths per annum.2, 3 High sensitive diagnosis method for early stage amebiasis is quite critical to prevent and cure this disease. To date, various serological tests have been used for the immune diagnosis of amebiasis, such as the indirect fluorescent antibody test (IFA) and enzyme-linked immunosorbent assay (ELISA).We have recently identified a 150-kDa surface antigen of E. histolytica as an intermediate subunit (Igl) of galactose and N-acetyl-D-galactosamine-inhibitable lectin.4, 5 In particular, it has been shown that the C-terminus of Igl (C-Igl, aa 603-1088) was an especially useful antigen for the serodiagnosis of amebiasis. ELISA using C-Igl is more specific than the traditional ELISA using crude antigen.6 However, the ELISA process usually takes several hours, which is still labor-intensive and requires experienced operators to perform. More economic and convenient filed diagnosis methods are still in need, especially for the developing countries with limited medical facilities.Among all the bioanalytical techniques, microfluidics has been attracting more and more attention because of its low reagent/power consumption, the rapid analysis speed as well as easy automation.7, 8, 9, 10, 11 Especially with the development of the fabrication technique, microfluidics chip can include valves, mixers, pumps, heating devices, and even micro sensors, so many traditional bioanalytical methods can be performed in the microfluidics. Qualitative and quantitative immune analysis on the microfluidic chip was successfully proved by plenty of research with improved sensitivity, shorten reaction time, and less sample consumption.8, 10, 11, 12, 13, 14, 15, 16, 17 Moreover, with the intervention of other physical, chemical, biology, and electronic technology, microfluidic technique has been successfully utilized in protein crystallization, protein and gene analysis, cell capture and culturing and analysis as well as in the rapid and quantitative detection of microbes.13, 14, 15, 16, 17, 18, 19, 20Herein, we report a new integrated microfluidic device, which is capable of rapid serodiagnosis of amebiasis with little sample consumption. The microfluidic device was fabricated from polydimethysiloxane (PDMS) following standard soft lithography.21, 22 The device was composed of two layers (shown in Figure Figure1)1) including upper fluidic layer (in green and blue) and bottom control layer (in red).Open in a separate windowFigure 1Structure illustration of microfluidic chip.To create the fluidic layer and the control layer, two different molds with different patterns have fabricated by photolithographic processes. The mold to create the fluidic channels was made by positive photoresist (AZ-50 XT), while the control pneumatic mold was made by negative photoresist (SU8 2025). For the chip fabrication, the fluidic layer is made from PDMS (RTV 615 A: B in ratio 5:1), and the pattern was transferred from the respective mold. The control layer is made from PDMS (RTV 615 A:B in ratio 20:1). The two layers were assembled and bonded together accurately, and there is elastic PDMS membrane about 30 μm thick between the fluidic layer channels and control layer.21, 22 The elastic membrane at the intersection can deform to block the fluid inside the fluidic channels, functioning as valves under the pressures introduced though control channels. There are two types of channels in fluidic layer, the rectangular profiled (in green, 200 μm wide, 35 μm thick) channel and round profiled channels (in blue, 200 μm wide, 25 μm center height). Because of the position of the valves on the fluidic channels, two types of valves (Figure (Figure2a)2a) were built, working as a standard valve and a sieve valve. The standard valves (on blue fluidic channels) can totally block the fluid because of the round profile of fluidic channel; the sieve valve can only half close because of the rectangular profile. The sieve valve can be used to trap the microspheres (beads) filled inside the green fluidic channels, while letting the fluid pass through. By this sieve valve, a micro column (in green) is constructed, where the entire ELISA reaction happens. The micrograph of the fabricated micro device is shown in Figure Figure2b.2b. The channels were filled with food dyes in different colors to show the relative positions of the channels. The pressures though different control channels are individually controlled by solenoid valves, connected to a computer through relay board. By programming the status (on/off) of various valves at different time periods, all the microfluidic chip operation can be digitally controlled by the computer in manual, semi-automatic, or automatic manner.Open in a separate windowFigure 2(a) Structure illustration of micro column, standard valve and sieve valve; (b) photograph of the microfluidic chip.To validate this device, 12 patient serum samples were collected. Sera from 9 patients (Nos. 1–9) with an amebic liver abscess or amebic colitis were used as symptomatic cases. The diagnosis of these patients was based on their clinical symptoms, ultrasound examination (liver abscess) and endoscopic or microscopic examination (colitis). We also identified the clinical samples using PCR amplification of rRNA genes.24 As negative control, sera obtained from 3 healthy individuals with no known history of amebiasis were mixed into pool sera. The serum was positive for E. histolytica with a titer of 1:64 (borderline positive), as determined by an indirect fluorescent-antibody (IFA) test.23, 24 In our previously study, the sensitivity and specificity of the recombinant C-Igl in the ELISA were 97% and 99%.6, 25 In the current study, the serodiagnosis of amebiasis was also examined by ELISA using C-Igl.26 The cut-off for a positive result was defined as an ELISA value > 3 SD above the mean for healthy negative controls27 (shown in Figure Figure3).3). The seropositivity to C-Igl was 100% in patients with amebiasis.Open in a separate windowFigure 3ELISA reactivity of sera from patients against C-Igl. ELISA plate was coated with 100 ng per well of C-Igl. Serum samples from patients and healthy controls were used at 1:400 dilutions. The dashed line indicates the cut-off value. Data are representative of results from three independent experiments.In the diagnosis process with microfluidic chip, the 4 micro immuno-columns filled with C-Igl-coated microspheres were the key components of the device. The C-Igl was prepared in E. coli as inclusion bodies. After expression, the recombinant protein was purified and analyzed by SDS-PAGE. The apparent molecular mass was 85 kDa.26The immune-reaction mechanism is illustrated in Figure Figure4.4. The anti-His monocolonal antibody was immobilized onto the microspheres (beads, 9 μm diameter) coated with protein A. The C-Igl was then immobilized onto the beads through the binding between the His tag and C-Igl. For the diagnosis, the microspheres immobilized with C-Igl and blocked by 5% BSA were preloaded into the columns for the rapid analysis of the patient serum samples. Generally, serum samples which were diluted 100 times were first loaded into the reaction column and incubated at room temperature for 5 min. After being washed by PBS buffer, FITC-conjugated goat anti-human polyclonal antibody was added into the column for 4 min incubation. The fluorescence image can be collected by the fluorescence microscope after the micro column was washed with PBS buffer. From loading diluted serum samples into column to collecting fluorescence images, the total time to complete the immunoassay is less than 10 min. The final fluorescence results were analyzed by Image Pro Plus 6.0.Open in a separate windowFigure 4Schematic representation of the ELISA in the chip.Different reaction conditions have been investigated to find the optimized ones. For each patient, 2 μl sample is enough for the analysis. The designed microfluidic chip with 4 micro columns is capable for 4 parallel analyses at the same time. More micro columns can be integrated into the device if more parallel tests are needed.Different incubating time for the diagnosis has also been investigated and no significant difference has been found for various time periods. It is enough to incubate the chip for only 5 min. The total diagnosis time for one sample is less than 10 min. The detection result appeared as the fluorescence intensity of the reaction column. As shown in Figure Figure5,5, the negative sample showed relatively low fluorescence intensity, because little FITC-conjugated goat anti-human polyclonal antibody could attach to the surface of microspheres; on the contrast, the positive sample showed much brighter fluorescence. The fluorescence intensity can be transferred to digital data (Table
SampleAverage scoresStandard deviation
133 790368
223 269271
339 598307
4778452
521 222197
638 878290
722 437227
836 295334
941 024396
Negative20032
Open in a separate windowOpen in a separate windowFigure 5ELISA on the chip. The signals were collected by CCD of microscope. A: negative sample; B and C: positive samples.For the heterogeneous immunoreactions, the immobilization of the immune molecules is essential for the reaction efficiency. Herein, we utilized micro columns filled with pre-modified microspheres (beads) instead of the direct surface modification for the ELISA analysis. Compared with the traditional method, diagnosis using the microfluidic device took less than 10 min with only 2 μl sample consumption and little reagent consumption. The high efficiency might be attributed to the high surface modification efficiency by using beads as well as the advantages from microfluidic device itself. The C-Igl modified microspheres can be easily prepared in 1 h and preloaded inside the micro device for convenient application. The device is made from standard soft lithography by PDMS and its throughput can be easily improved by adding more micro columns into the microfluidic device in an economic manner, which is perfect for the onsite rapid and cheap diagnosis of amebiasis. Similar methodologies can be developed for diagnosis of other infectious disease, especially for the developing countries with very limited medical facilities.  相似文献   
4.
Muscle cross-sectional areas and performance power of limbs and trunk in the rowing motion     
Tachibana K  Yashiro K  Miyazaki J  Ikegami Y  Higuchi M 《Sports biomechanics / International Society of Biomechanics in Sports》2007,6(1):44-58
Although it is clear that rowers have a large muscle mass, their distribution of muscle mass and which of the main motions in rowing mediates muscle hypertrophy in each body part are unclear. We examine the relationships between partial motion power in rowing and muscle cross-sectional area of the thigh, lower back, and upper arms. Sixty young rowers (39 males and 21 females) participated in the study. Joint positions and forces were measured by video cameras and rowing ergometer software, respectively. One-dimensional motion analysis was performed to calculate the power of leg drive, trunk swing, and arm pull motions. Muscle cross-sectional areas were measured using magnetic resonance imaging. Multiple regression analyses were carried out to determine the association of different muscle cross-sectional areas with partial motion power. The anterior thigh best explained the power demonstrated by leg drive (r2 = 0.508), the posterior thigh and lower back combined best explained the power demonstrated by the trunk swing (r2 = 0.493), and the elbow extensors best explained the power demonstrated by the arm pull (r2 = 0.195). Other correlations, such as arm muscles with leg drive power (r2 = 0.424) and anterior thigh with trunk swing power (r2 = 0.33 5), were also significant. All muscle cross-sectional areas were associated with rowing performance either through the production of power or by transmitting work. The results imply that rowing motion requires a well-balanced distribution of muscle mass throughout the body.  相似文献   
5.
Rapid microfluidic immunoassay for surveillance and diagnosis of Cryptosporidium infection in human immunodeficiency virus-infected patients     
Li Zhang  Yongfeng Fu  Wenwen Jing  Qing Xu  Wang Zhao  Meng Feng  Hiroshi Tachibana  Guodong Sui  Xunjia Cheng 《Biomicrofluidics》2015,9(2)
Cryptosporidiosis has been reported to be associated with HIV/acquired immune deficiency syndrome, which greatly reduces the quality of life and shortens the life expectancy of HIV-infected patients. In order to properly treat the infected patients, accurate and automatic diagnostic tools need to be developed. In this study, a novel microfluidic immunochip system was presented for the surveillance and the rapid detection of Cryptosporidium infection in 190 HIV-infected patients from Guangxi, China, using the P23 antigen of Cryptosporidium. The procedure of detection can be completed within 10 min with 2 μl sample consumption. The system also was evaluated using the standard ELISA method. Among 190 HIV-infected individuals, the rate of P23 positivity was 13.7%. Seropositivity in HIV-infected individuals was higher in female patients. The seropositivity to P23 was higher in HIV-infected individuals with high viral load, although the difference was statistically insignificant. Significantly higher Cryptosporidium seropositivity was observed in HIV-infected individuals with a CD4+ T-cell count of <200 cells/μl than in those with ≥200 cells/μl. Our results also demonstrate that a lower CD4+ T-cell count may reflect an increased accumulated risk for cryptosporidiosis. The detection system was further validated using the standard ELISA method and good correlation between the two methods was found (r = 0.80). Under the same sensitivity, this new microfluidic chip device had a specificity of 98.2%. This developed system may provide a powerful platform for the fast screening of Cryptospordium infection in HIV-infected patients.  相似文献   
6.
Higher reliability and closer relationship between open-field test measures on aggregation data     
Toshiaki Tachibana 《Learning & behavior》1985,13(4):345-348
Eighty male rats were tested in an open field. Correlation coefficients between aggregated test days were larger than those between nonaggregated test days, indicating that aggregation across days can enhance the reliability of scores in the open-field test. Also, absolute values of correlation coefficients among the seven open-field test measures based on the aggregated data were generally larger than those based on nonaggregated data, indicating that the correlation among measures may be closer than previously assumed on the basis of nonaggregated data. Issues concerning appropriate aggregation and limitations of aggregation are discussed. The technique of aggregation is recommended as a routine procedure in the analysis of open-field test results, because of the enhanced reliability obtained.  相似文献   
7.
Job search motivation of part-time or unemployed Japanese college graduates     
Toshiaki Shirai  Hideo Shimomura  Tomotsugu Kawasaki  Tomoko Adachi  Yosuke Wakamatsu 《International Journal for Educational and Vocational Guidance》2013,13(2):95-114
We clarify how individuals actively interact with socio-cultural contexts to attain regular employment in Japan. Based on a large sample (N = 3,512) of part-time employed and unemployed college graduates (23–39 years old), we found that: Career decision making self-efficacy predicted job search; a lack of both hope and fulfillment motivated job search, while having hope promoted it; wishing for perfect vocation and being free from both inclination towards personal interests and passivity motivated job search. Clients’ desire for “a perfect vocation,” should not necessarily be considered as a career barrier but, perhaps, rather as an asset for motivating job search. Counselors should seek to better understand the influence of clients’ socio-cultural contexts on their career attitudes.  相似文献   
8.
The open-field test: An approach from multivariate analysis     
Toshiaki Tachibana 《Learning & behavior》1980,8(3):465-467
The validity of open-field test measures was assessed by simple and multiple regression analysis, taking the difference in experience of a novel situation as the external criterion. Ambulation in the first 1 min, rearing, latency to urination, and urination score were valid indexes of a new operational concept presented in this study, and they were also reliable. The weighting coefficients were obtained for the reduction of multiple measure scores to a single score that represents the concept.  相似文献   
9.
ASPEC: 亚洲科学论文摘录语料库          下载免费PDF全文
Toshiaki Nakazaw  Manabu Yaguchi  Kiyotaka Uchimoto  Masao Utiyam  Eiichiro Sumit  Sadao Kurohashi  Hitoshi Isahar  何彦青  刘建辉 《情报工程》2017,3(3):040-046
本文详细介绍了ASPEC(亚洲科学论文摘录语料库)。作为首个大规模的科学论文领域内的平行语料库,ASPEC 是由日- 中机器翻译项目于 2006 年至2010 年间利用科技促进专用协作基金构建起来的。它包含约300 万条平行语句的日- 英科学论文摘要语料库(ASPEC-JE)和约68万条平行语句的中- 日科学论文摘录语料库(ASPEC-JC)。ASPEC 被用作机器翻译评测研讨会WAT(亚洲翻译研讨会)的官方数据集。  相似文献   
10.
Nonadmirable moral exemplars and virtue development     
Koji Tachibana 《Journal of moral education》2019,48(3):346-357
ABSTRACT

Linda Zagzebski’s exemplarist moral theory claims that admiration for a person is a necessary condition for her to be a moral exemplar. I argue that this claim is empirically unsupported. I provide two counterexamples, astronauts and brain data. I demonstrate that they play the role of exemplars well but receive no admiration and, accordingly, are entitled to be called nonadmirable moral exemplars. I conclude that my argument suggests why Aristotle, distinct from Zagzebski, does not emphasise the role of the praiseworthiness of virtue in his theory of virtue development in the Nicomachean Ethics.  相似文献   
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