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1.
Vahid Aryadoust Sanaz Akbarzadeh Sara Akbarzedeh 《Asia Pacific Journal of Education》2011,31(1):51-64
The Multidimensional School Anger Inventory–Revised (MSAI-R) is a measurement tool to evaluate high school students' anger. Its psychometric features have been tested in the USA, Australia, Japan, Guatemala, and Italy. This study investigates the factor structure and psychometric quality of the Persian version of the MSAI-R using data from an administration of the inventory to 585 Iranian high school students. The study adopted the four-factor underlying structure of high school student anger derived through factor analysis in previous validation studies, which consists of: School Hostility, Anger Experience, Positive Coping, and Destructive Expressions. Confirmatory factor analysis of this four-factor model indicated that it fit the data better than a one-factor baseline model, although the fit was not perfect. The Rasch model showed a very high internal consistency among items, with no item misfitting; however, our results suggest that to represent the construct sufficiently some items should be added to Positive Coping and Destructive Expression. This finding is in agreement with Boman, Curtis, Furlong, and Smith's Rasch analysis of the MSAI-R with an Australian sample. Overall, the results from this study support the psychometric features of the Persian MSAI-R. However, results from some test items also point to the dangers inherent in adapting the same test stimuli to widely divergent cultures. 相似文献
2.
This study considers merging dynamical networks (relative sensing networks in this paper) in terms of a stability margin criterion. The main motivation of this consideration is that merging can cause a significant drop in the stability margin of merged network with respect to the original networks initially with ample stability margins. In this paper, various types of network merging (i.e. undirected/directed homogeneous/heterogeneous dynamical network merging via one-way/two-way links) are analysed to show their effects on the stability margin. In particular, it is shown that (1) merging with one-way links yields the stability margin less than the original networks’; (2) merging undirected homogeneous networks with two-way links results in a stability margin being at least a quantity solely characterized by the positive realness (PRness) of SISO (Single-Input-Single-Output) local dynamics; (3) the quantity depends both on the PRness of SISO local dynamics and the eigenvalues of Laplacian matrix, in case of merging directed homogeneous networks with two-way links; (4) two-way merging using multiple nodes may allow for a large increase in the stability margin; and (5) merging heterogeneous networks may be simply treated as merging homogeneous networks by exploiting the design of link dynamics. Several numerical results are presented to show their consistency with the performed analysis. 相似文献
3.
Anil Haraksingh Thilsted Vahid Bazargan Nina Piggott Vivien Measday Boris Stoeber 《Biomicrofluidics》2012,6(4)
A flow redirection and single cell immobilization method in a microfluidic chip is presented. Microheaters generated localized heating and induced poly(N-isopropylacrylamide) phase transition, creating a hydrogel that blocked a channel or immobilized a single cell. The heaters were activated in sets to redirect flow and exchange the fluid in which an immobilized cell was immersed. A yeast cell was immobilized in hydrogel and a 4′,6-diamidino-2-phenylindole (DAPI) fluorescent stain was introduced using flow redirection. DAPI diffused through the hydrogel and fluorescently labelled the yeast DNA, demonstrating in situ single cell biochemistry by means of immobilization and fluid exchange.The ability to control microfluidic flow is central to nearly all lab-on-a-chip processes. Recent developments in microfluidics either include microchannel based flow control in which microvalves are used to control the passage of fluid,1 or are based on discrete droplet translocation in which electric fields or thermal gradients are used to determine the droplet path.2, 3 Reconfigurable microfluidic systems have certain advantages, including the ability to adapt downstream fluid processes such as sorting to upstream conditions and events. This is especially relevant for work with individual biomolecules and high throughput cell sorting.4 Additionally, reconfigurable microfluidic systems allow for rerouting flows around defective areas for high device yield or lifetime and for increasing the device versatility as a single chip design can have a variety of applications.Microvalves often form the basis of flow control systems and use magnetic, electric, piezoelectric, and pneumatic actuation methods.5 Many of these designs require complicated fabrication steps and can have large complex structures that limit the scalability or feasability of complex microfluidic systems. Recent work has shown how phase transition of stimuli-responsive hydrogels can be used to actuate a simple valve design.6 Beebe et al. demonstrated pH actuated hydrogel valves.7 Phase transition of thermosensitive poly(N-isopropylacrylamide) (PNIPAAm) using a heater element was demonstrated by Richter et al.8 Phase transition was also achieved by using light actuation by Chen et al.9 Electric heating has shown a microflow response time of less than 33 ms.11 Previous work10 showed the use of microheaters to induce a significant shift in the viscosity of thermosensitive hydrogel to block microchannel flow and deflect a membrane, stopping flow in another microchannel. Additionally, Yu et al.12 demonstrated thermally actuated valves based on porous polymer monoliths with PNIPAAm. Krishnan and Erickson13 showed how reconfigurable optically actuated hydrogel formation can be used to dynamically create highly viscous areas and thus redirect flow with a response time of ～ 2?s. This process can be used to embed individual biomolecules in hydrogel and suppress diffusion as also demonstrated by others.15, 16 Fiddes et al.14 demonstrated the use of hydrogels to transport immobilized biomolecules in a digital microfluidic system. While the design of Krishnan and Erickson is highly flexible, it requires the use of an optical system and absorption layer to generate a geometric pattern to redirect flow.This paper describes the use of an array of gold microheaters positioned in a single layer polydimethylsiloxane (PDMS) microfluidic network to dynamically control microchannel flow of PNIPAAm solution. Heat generation and thus PNIPAAm phase transition were localized as the microheaters were actuated using pulse width modulation (PWM) of an applied electric potential. Additionally, hydrogel was used to embed and immobilise individual cells, exchange the fluid parts of the microfluidic system in order to expose the cells to particular reagents to carry out an in situ biochemical process. The PDMS microchannel network and the microheater array are shown in Figure Figure11.Open in a separate windowFigure 1A sketch of the electrical circuit and a microscope image of the gold microheaters and the PDMS microchannels. The power to the heaters was modulated with a PWM input through a H-bridge. For clarity, the electrical circuit for only the two heaters with gelled PNIPAAm is shown (H1 and V2). There are four heaters (V1-V4) in the “vertical channels” and three heaters (H1-H3) in the “horizontal” channel.The microchannels were fabricated using a patterned mould on a silicon wafer to define PDMS microchannels, as described by DeBusschere et al.17 and based on previous work.10 A 25 × 75 mm glass microscope slide served as the remaining wall of the microchannel system as well as the substrate for the microheater array. The gold layer had a thickness of 200 nm and was deposited and patterned using E-beam evaporation and photoresist lift-off.21 The gold was patterned to function as connecting electrical conductors as well as the microheaters.It was crucial that the microheater array was aligned with an accuracy of ～ 20μm with the PDMS microchannel network for good heat localization. The PDMS and glass lid were treated with plasma to activate the surface and alignment was carried out by mounting the microscope slide onto the condenser lens of an inverted microscope (TE-2000 Nikon Instruments). While imaging with a 4× objective, the x, y motorized stage aligned the microchannels to the heaters and the condenser lens was lowered for the glass substrate to contact the PDMS and seal the microchannels.Local phase transition of 10% w/w PNIPAAm solution in the microchannels was achieved by applying a 7 V potential through a H-bridge that received a PWM input at 500 Hz which was modulated using a USB controller (Arduino Mega 2650) and a matlab (Mathworks) GUI. The duty cycle of the PWM input was calibrated for each microheater to account for differences in heater resistances (25?Ω to 52?Ω) due to varying lengths of on-chip connections and slight fabrication inconsistencies, as well as for different flow conditions during device operation. Additionally, thermal cross-talk between heaters required decreasing the PWM input significantly when multiple heaters were activated simultaneously. This allowed confining the areas of cross-linked PNIPAAm to the microheaters, allowing the fluid in other areas to flow freely.By activating the heaters in sets, it was possible to redirect the flow and exchange the fluid in the central area. Figure Figure22 demonstrates how the flow direction in the central microchannel area was changed from a stable horizontal flow to a stable vertical flow with a 3 s response time, using only PNIPAAm phase transition. Constant pressures were applied to the inlets to the horizontal channel and to the vertical channels. Activating heaters V1-4 (Figure (Figure2,2, left) resulted in flow in the horizontal channel only. Likewise, activating heaters H1 and H2 allowed for flow in the vertical channel only. In this sequence, the fluid in the central microchannel area from one inlet was exchanged with fluid from the other inlet. Additionally, by activating heater H3, a particle could be immobilised during the exchange of fluid as shown in Figure Figure33 (top).Open in a separate windowFigure 2Switching between fluid from the horizontal and the vertical channel using hydrogel activation and flow redirection with a response time of 3 s. A pressure of 25 mbar was applied to the inlet of the horizontal channel and a pressure of 20 mbar to the vertical channel. The flow field was determined using particle image velocimetry, in which the displacement of fluorescent seed particles was determined from image pairs generated by laser pulse exposure. Processing was carried out with davis software (LaVision).Open in a separate windowFigure 3A series of microscope images near heater H3 showing: (1a)-(1c) A single yeast cell captured by local PNIPAAm phase transition and immobilized for 5 min before being released. (2a) A single yeast cell was identified for capture by embedding in hydrogel. (2b) The cell as well as the hydrogel displayed fluorescence while embedded due to the introduction of DAPI in the surrounding region. (2c) The diffusion of DAPI towards the cell as the heating power of H3 is reduced after 15 min, showing a DAPI stained yeast cell immobilized.Particle immobilisation in hydrogel and fluid exchange in the central area of the microfluidic network were used to carry out an in situ biochemical process in which a yeast cell injected through one inlet was stained in situ with a 4′,6-diamidino-2-phenylindole (DAPI) solution (Invitrogen), which attached to the DNA of the yeast cell.18 A solution of yeast cells with a concentration of 5 × 107cells/ml suspended in a 10% w/w PNIPAAm solution was injected through the horizontal channel. A solution of 2μg/l DAPI in a 10% w/w PNIPAAm solution was injected through the vertical channel. A single yeast cell was identified and captured near the central heater, and by deactivating the heaters in the vertical channel, DAPI solution was introduced in the microchannels around the hydrogel. After immobilising the cell for 15 min, the heater was deactivated, releasing the cell in the DAPI solution. This process is shown in Figure Figure33 (bottom). The sequence of the heater activation and deactivation in order to immobilize the cell and exchange the fluid is outlined in the supplementary material.21Eriksen et al.15 demonstrated the diffusion of protease K in the porous hydrogel matrix,19 and it was therefore expected that DAPI fluorescent stain (molecular weight of 350 kDa, Ref. 20) would also diffuse. DAPI diffusion is shown in Figure 3(2b) in which the yeast cell shows fluorescence while embedded in the hydrogel. The yeast cell was released by deactivating the central heater and activating all the others to suppress unwanted flow in the microchannel. As a result, the single cell was fully immersed in the DAPI solution. Immobilization of a single cell allows for selection of a cell that exhibits a certain trait and introduction of a new fluid while maintaining the cell position in the field of view of the microscope such that a biochemical response can be imaged continuously.In summary, a microfluidic chip capable of local heating was used to induce phase transition of PNIPAAm to hydrogel, blocking microchannel flow, and thereby allowing for reconfigurable flow. Additionally, the hydrogel was used to embed and immobilise a single yeast cell. DAPI fluorescent stain was introduced using flow redirection, and it stained the immobilized cell, showing diffusion into the hydrogel. The versatile design of this microfluidic chip permits flow redirection, and is suitable to carry out in situ biochemical reactions on individual cells, demonstrating the potential of this technology for forming large-scale reconfigurable microfluidic networks for biochemical applications. 相似文献
4.
Vahid Aryadoust 《Pedagogies: An International Journal》2017,12(2):151-179
This study adapts Levels 1 and 2 of Kirkpatrick’s model of training evaluation to evaluate learning outcomes of an English as a second language (ESL) paragraph writing course offered by a major Asian university. The study uses a combination of surveys and writing tests administered at the beginning and end of the course. The survey evaluated changes in students’ perception of their skills, attitude, and knowledge (SAK), and the writing tests measured their writing ability. Rasch measurement was applied to examine the psychometric validity of the instruments. The measured abilities were successively subjected to path modeling to evaluate Levels 1 and 2 of the model. The students reported that the module was enjoyable and useful. In addition, their self-perceived level of skills and knowledge developed across time alongside their writing scores but their attitude remained unchanged. Limitations of Kirkpatrick’s model as well as lack of solid frameworks for evaluating educational effectiveness in applied linguistics are discussed. 相似文献
5.
Vahid Aryadoust 《教育心理学》2016,36(10):1742-1770
This study sought to examine the development of paragraph writing skills of 116 English as a second language university students over the course of 12 weeks and the relationship between the linguistic features of students’ written texts as measured by Coh-Metrix – a computational system for estimating textual features such as cohesion and coherence – and the scores assigned by human raters. The raters’ reliability was investigated using many-facet Rasch measurement (MFRM); the growth of students’ paragraph writing skills was explored using a factor-of-curves latent growth model (LGM); and the relationships between changes in linguistic features and writing scores across time were examined by path modelling. MFRM analysis indicates that despite several misfits, students’ and raters’ performances and scale’s functionality conformed to the expectations of MFRM, thus providing evidence of psychometric validity for the assessments. LGM shows that students’ paragraph writing skills develop steadily during the course. The Coh-Metrix indices have more predictive power before and after the course than during it, suggesting that Coh-Metrix may struggle to discriminate between some ability levels. Whether a Coh-Metrix index gains or loses predictive power over time is argued to be partly a function of whether raters maintain or lose sensitivity to the linguistic feature measured by that index in their own assessment as the course progresses. 相似文献
6.
Vahid Mahmoudi-Gahrouei Mansoor Tavakoli Doug Hamman 《Asia Pacific Education Review》2016,17(4):581-597
Professional identity surfaces repeatedly as an important underlying factor in teacher development. A sequential explanatory mixed methods design was used to investigate identity development in terms of teachers’ expected and feared possible selves. Teachers (n = 120) representing three career groups (prospect, new, and experienced) participated. The findings reveal differences in possible selves which are detectable based on career group. New teachers exhibited a greater focus on task self-concepts; prospective teachers expressed both task and quality possible selves; and experienced teachers expressed identities more concerned about the quality-focused ones. Our findings also reveal a potential transition path in professional identity development. 相似文献
7.
Lim Nikki Zhi Li Zakaria Azrifah Aryadoust Vahid 《Education and Information Technologies》2022,27(5):6125-6155
Education and Information Technologies - Digital storytelling (DST) is a novel approach that uses modern computer technology to amplify language learning and teaching. The present study aims to... 相似文献
8.
Graph transformation systems have become a general formal modeling language to describe many models in software development process. Behavioral modeling of dynamic systems and model-to-model transformations are only a few examples in which graphs have been used to software development. But even the perfect graph transformation system must be equipped with automated analysis capabilities to let users understand whether such a formal specification fulfills their requirements. In this paper, we present a new solution to verify graph transformation systems using the Bogor model checker. The attributed graph grammars （AGG）-like graph transformation systems are translated to Bandera intermediate representation （BIR）, the input language of Bogor, and Bogor verifies the model against some interesting properties defined by combining linear temporal logic （LTL） and special-purpose graph rules. Experimental results are encouraging, showing that in most cases our solution improves existing approaches in terms of both performance and expressiveness. 相似文献
9.
A link addition problem is considered for a directed linear network in order to improve the network robustness. The robustness of network is quantified through a well-known measure of stability margin. Optimal identification of links such that the stability margin is maximized by their addition, requires solving a complex combinatorial problem. Here two efficient methods are proposed for suboptimal link addition by exploiting the sensitivity being embedded in the derivative of singular value and Lagrange multiplier. Numerical tests are presented to demonstrate the efficacy of the proposed methods. 相似文献
10.
Vahid Esfahanian 《Journal of The Franklin Institute》2011,348(7):1208-1230
A locally power-law preconditioning algorithm is developed. This is applied to compute incompressible inviscid, steady-state, non-cavitating and cavitating flows. The preconditioning parameters are adapted automatically from the pressure of computational domain. This method suggests better convergence rates rather than the standard artificial compressibility and the standard preconditioning method. Single-fluid Euler equations, cast in their conservative form, along with the barotropic cavitation model are employed. The cell-centred Jameson's finite volume discretization technique is used to solve the preconditioned governing equations. The stabilization is achieved via the second and fourth, order artificial dissipation scheme. Explicit four-stage Runge-Kutta time integration is applied to find the steady-state condition. In this paper, the method is assessed through simulations of incompressible inviscid, steady-state, non-cavitating and cavitating flows over a 2D NACA0012 and a 2D NACA66(MOD)+a=0.8 hydrofoil section. The results show satisfactory agreement with others numerical and experimental works in pressure distribution and hydrodynamic forces. Using the power-law preconditioner decreases the convergence rate significantly. In addition, information such as the effects of the new locally power-law preconditioner, the effects of the artificial dissipation terms, and the effects of the artificial compressibility parameter, on convergence speed and solution accuracy is highlighted. 相似文献