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1.
Lakshmi Lavanya Reddy Swarup A. V. Shah Alpa J. Dherai Chandrashekhar K. Ponde Tester F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2016,31(1):87-92
Acute coronary syndrome (ACS) is a term for a range of clinical signs and symptoms suggestive of myocardial ischemia. It results in functional and structural changes and ultimately releasing protein from injured cardiomyocytes. These cardiac markers play a major role in diagnosis and prognosis of ACS. This study aims to assess the efficacy of heart type fatty acid binding protein (h-FABP) as a marker for ACS along with the routinely used hs-TropT. In our observational study, plasma h-FABP (cut-off 6.32 ng/ml) and routinely done hs-Trop T (cutoff 0.1 and 0.014 ng/ml) were estimated by immunometric laboratory assays in 88 patients with acute chest pain. Based on the clinical and laboratory test findings the patients were grouped into ACS (n = 41) and non-ACS (n = 47). The diagnostic sensitivity, specificity, NPV, PPV and ROC curve at 95 % CI were determined. Sensitivity of hs-TropT (0.1 ng/ml), hs-TropT (0.014 ng/ml) and h-FABP were 53, 86 and 78 % respectively and specificity for the same were 98, 73 and 70 % respectively. Sensitivity, specificity and NPV calculated for a cut-off combination of hs-TropT 0.014 ng/ml and h-FABP was 100, 51 and 100 % respectively. These results were substantiated by ROC analysis. Measurement of plasma h-FABP and hs-TropT together on admission appears to be more precise predictor of ACS rather than either hs-Trop T or h-FABP. 相似文献
2.
Karen M. D'Souza Tester F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2007,22(1):37-44
This study was aimed at isolating, in its pure form, and characterizing the sarcoplasmic reticulum from caprine (Capra hircus) heart. The sarcoplasmic reticulum from thirty caprine heart ventricular homogenates was isolated and purified. It was characterized
on the basis of both, its protein and lipid composition. The protein content was 142±10 mg/g of tissue. Ca2+-ATPase activity equaled 3.75±1.06mmol Pi/mg protein/min while the uptake rate was 24±1.14 nmol/mg protein/min. 205kD, 110kD,
90kD, 84kD, 66kD, 55kD and 29kD molecular weight proteins were seen on an SDS polyacrylamide gel. Triglyceride, Cholesterol
and Phospholipids (phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, sphingomyelin and phosphatidylserine)
were present in increasing order of their concentration. Long chain fatty acids predominated over the unsaturated ones. The
ryanodine receptor displayed two binding sites for ryanodine. Characterisation encompassing the above biochemical aspects
of normal caprine cardiac sarcoplasmic reticulum was thus achieved after isolating it in the pure form. 相似文献
3.
Seema P. Todur Tester F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2013,28(2):116-123
There is an increasing interest to understand the molecular basis of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) subfractions and their association with coronary artery disease (CAD). The formation of these subfractions is greatly influenced by hepatic lipase (HL) and cholesteryl ester transfer protein (CETP) enzymes. To identify genetic markers influencing LDL and HDL subfractions and their role in CAD we performed a case–control genetic association study on 117 healthy controls and 119 angiographically verified CAD patients. Biochemical analysis was performed using standard assays. HDL-C and LDL-C subfractions were estimated using precipitation methods. Genotyping of C-514T (rs1800588) in the LIPC gene for HL and I405V (rs5882) in the CETP gene was done using PCR-based restriction enzyme analysis and sequencing. Both the polymorphisms were not associated with CAD. The C-514T was associated with increased HDL3-C levels in controls (P = 0.049). The I405V polymorphism was found to be associated with low levels of small dense, LDL (P = 0.038). A multiple regression analysis showed that the effects were dependent on gender and triglyceride levels. We conclude that these polymorphisms are not associated with CAD but are important determinants of HDL-C and small dense LDL particles in our population. 相似文献
4.
Influence of blood specimen collection method on various preanalytical sample quality indicators 总被引:1,自引:0,他引:1
Tester F. Ashavaid Sucheta P. Dandekar Bhamini Keny Vishaal R. Bhambhwani 《Indian journal of clinical biochemistry : IJCB》2008,23(2):144-149
Preanalytical errors contribute to a large proportion of total laboratory errors. In order to achieve continuous laboratory
improvement, it is important to focus on all phases of patient specimen testing i.e. preanalytical, analytical and post-analytical.
With large variations in the way venous blood specimens are collected using diverse devices in the country, the effect of
such practices on specimen quality is not known. The purpose of this study was to monitor fourteen specimen preanalytical
quality indicators in order to compare the usage of evacuated blood collection devices with needle and syringe open collection
using either disposable tubes or re-washed glass vials. The study involved 26638 patient specimens assessed over a period
of 6 months. The results demonstrated that evacuated closed blood collection resulted in an approximate 100-fold reduction
in the incidence of hemolysis in samples. Similarly, there was a 200-fold reduction in incidence of insufficient specimen
quantity while using evacuated collection system. It was also found that incidence of specimen contamination, improper volume
of sample collected, and specimen spillage was also lower when the evacuated collection system was used. Further, it was also
observed that the facility with a laboratory information system demonstrated much lower specimen identification and related
errors. The observed results clearly demonstrate that the usage of the evacuated blood collection system resulted in improvement
of preanalytical specimen quality as compared to needle and syringe usage. 相似文献
5.
Ashavaid T. F. Altaf A. K. Nair K. G. 《Indian journal of clinical biochemistry : IJCB》2000,15(1):11-16
Modification of platelet proteins by free radicals and glycation has been studied in the present work, as anin vitro model. The results of the two parameters, SDS-PAGE and carbonylation study are quite corroborative. We observed that the
inducers like ferrous sulphate, ascorbate (mainly in supraphysiological concentration) and glucose attack the protein in a
dose dependent manner, of which ferrous sulphate is most potent. Proteins from aged and degenerative conditions like malignancy
and diabetes mellitus have suffered greater damage than normal adult and foetal proteins. The individual life expectancy in
terms of biological versus chronological age may also be worked out from the individual stress level. 相似文献
6.
Tester F. Ashavaid Ph.D. FACB Neena S. Kumbhat 《Indian journal of clinical biochemistry : IJCB》2005,20(1):72-79
Cardiac mitochondria provide energy for the contraction/relaxation cycle. The aim of our study was to isolate and characterize
mitochondria from Caprine hearts under control and in-vitro induced ischemia. A decrease in activities of all the enzymes
was observed in the ischemic models. Further characterization of proteins was done by SDS-PAGE and BN-PAGE. Lipids have been
characterized by analyzing the phospholipids by HPTLC and fatty acids by GLC in both groups. Our results indicated that injury
occurs early in the course of ischemia and progresses during ischemia. TBARS and carbonyl content have also been measured.
The in-vitro effects of fatty acids have been studied on the enzymes and complexes of mitochondria. 相似文献
7.
Tester F. Ashavaid Farah F. Eghlim Kavita K. Shalia Kappiareth G. Nair 《Indian journal of clinical biochemistry : IJCB》2003,18(2):106-110
Determination of plasma total homocysteine by high-pressure liquid chromatography (HPLC) usually requires reduction of protein
bound or free homocysteine-disulphides into thiols by a reducing agent and the liberated thiols are then derivatized by a
fluorescent marker. In this study we have standardized the HPLC method for homocysteine measurement using dithiothreitol (DTT)
as reductant.
The results of plasma total homocysteine values obtained by HPLC were compared with IMx method. The difference between the
two means was statistically insignificant [P=0.616847 (two tail)] Linear regression analysis showed strong correlation between
the two methods (r=0.983). Using this method we have analyzed 132 controls and 130 Coronary Heart Disease (CHD) patients for
plasma total homocysteine, wherein, the mean plasma total homocysteine levels were 10.51±8.36 and 11.51±10.06 μmol/L respectively.
Our research study suggests that DTT method is a simple and inexpensive assay for homocysteine determination in human plasma
for research application. 相似文献
8.
Ashavaid T. F. Shalia K. K. Nair K. G. Dalal J. J. 《Indian journal of clinical biochemistry : IJCB》2000,15(1):1-5
The levels of cytotoxic aldehydic products in different culinary oils, with or without thermal stress, (routine domestic or
commercial frying) were determined by thiobarbituric acid method. The results showed that (i) thiobarbituric acid reactivity
was much higher in edible oils rich in polyunsaturated fatty acids than those rich in saturated fatty acids or monounsaturated
fatty acids, even without thermal stress, (ii) the lipid peroxide levels were in proportion to the duration of thermal stress,
(iii) nature of the container used (steel, iron or teflon-coated) had no significant effect on the extent of lipid peroxidation
under identical conditions of thermal stress and (iv) thermally stressed oils collected from hotels and roadside caterers
contained higher levels of cytotoxic aldehydic products, when compared to oils thermally stressed under domestic frying conditions.
These results suggest that dietary ingestion of thermally or autoxidatively stressed polyunsaturated fatty acid rich culinary
oils is more harmful compared with those similarly treated oils rich in saturated fatty acids and monounsaturated fatty acids. 相似文献
9.
Tester F. Ashavaid Seema P. Todur Kappiareth G. Nair 《Indian journal of clinical biochemistry : IJCB》2002,17(1):83-93
Apolipoprotein E genotypes and lipid and lipoprotein levels were determined in hypercholesterolemic and angiographically vertified
CHD subjects and compared against 90 normolipidemic controls. The ε4 allele was significantly prevalent in the hypercholesterolemic
and CHD subjects. Significant increase in total cholesterol levels in apo ε4 containing subjects were observed in the hypercholesterolemic
and CHD group. The study suggests that the ε4 allele by influencing the lipid levels could act as a risk factor for CHD. 相似文献
10.