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Bharti Jain J. Kumarasamy Chandrakala Gholve Savita Kulkarni M. G. R. Rajan 《Indian journal of clinical biochemistry : IJCB》2017,32(2):193-199
Serum thyroglobulin (Tg) and thyroid stimulating hormone (TSH) measurements have evolved as important analytes for monitoring the prognosis of patients with differentiated thyroid cancer, post-thyroidectomy. Individual analyte immunoassay is the current practice in clinical pathology, but the simultaneous assay for all relevant analytes for a given disease, can reduce assay costs, improve patient compliance and give the clinician more information for an unequivocal diagnosis. Microarray immunoassay (MI) can achieve this goal and, hence, we have developed and validated a immuno-radiometric MI for quantitation of serum TSH and Tg by using highly micro-porous polycarbonate (PC) track-etched membranes (TEM) to immobilize the monoclonal anti-TSH and polyclonal anti-Tg antibodies in ~1 mm diameter spots. Non-competitive immunoassays were performed using mixture of 125I labeled monoclonal anti-TSH and anti-Tg antibodies. Phosphorimager was used to quantify the bound radioactivity. TSH and Tg were detected with detection limit of 0.07 µIU/ml and 0.13 ng/ml respectively, which is lower than the clinically required cut-off level. The assay showed: acceptable intra-assay precision within 20 % and recovery in the range of 76–111.2 %. MI compared well with the established immunoradiometric assay (IRMA) with r = 0.98, p < 0.01 (n = 41). No cross-reactivity was seen between the immobilized antibodies. Although two hormones are addressed in this report, MI using PC TEM and isotopic/non-isotopic tracers has the potential for highly automated multiplexed analysis. 相似文献
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This two-part article considers certain fundamental symmetries of nature, namely the discrete symmetries of parity (P), charge conjugation (C) and time reversal (T), and their possible violation. Recent experimental results are discussed in some depth. In this second part, we discussCP andT violation and arrive at a synthesis. 相似文献
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The electroviscous flow at low Reynolds number through a two-dimensional slit contraction with electric double-layer overlap is investigated numerically for cases where the permittivity of the wall material is significant in comparison with the permittivity of the liquid. The liquid-solid interface is assumed to have uniform surface-charge density. It is demonstrated that a finite wall permittivity has a marked effect on the distribution of ions in and around the contraction, with a significant build-up of counter-ions observed at the back-step. The development length of the flow increases substantially as the wall permittivity becomes significant, meaning that the electric double-layers require a longer distance to develop within the contraction. Consequently, there is a corresponding decrease in the hydrodynamic and electro-potential resistance caused by the contraction. The effect of wall-region width on the flow characteristics is also quantified, demonstrating that the development length increases with increasing wall-region width for widths up to 5 channel widths. 相似文献
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Suchanda Sahu MBBS MD Rajinder Chawla Bharti Uppal 《Indian journal of clinical biochemistry : IJCB》2005,20(2):54-61
Current recommendations of the Adult Treatment Panel and Adolescents Treatment Panel of National Cholesterol Education Program
make the low-density lipoprotein cholesterol (LDL-C) levels in serum the basis of classification and management of hypercholesterolemia.
A number of direct homogenous assays based on surfactant/solubility principles have evolved in the recent past. This has made
LDL-C estimation less cumbersome than the earlier used methods. Here we compared one of the direct homogenous assays with
the widely used Friedewald’s method of estimation of LDL-C to see the differences and correlation. We used direct homogenous
assay kit to estimate serum LDL-C and high-density lipoprotein cholesterol (HDL-C). Serum Triglyceride (TG) and Total Cholesterol
(TC) was estimated and using Friedewald’s formula LDL-C was calculated. The LDL-C level obtained by both methods in 893 fasting
serum samples were compared. The statistical methods used were paired t-test and Pearson’s correlation.
There was significant difference in the mean LDL-C levels obtained by the two methods at the TG levels <200 mg/dl (p<0.02)
and TC levels >150 mg% (p<0.001). The correlation coefficient (r) between Friedewald’s and direct assay estimation was 0.88.
Friedewald’s method classified 23.5 % of patients as high cardiac risk whereas there were 17.58% by direct assay.
Both had good correlation even though the serum triglyceride and total cholesterol levels affect the difference in LDL-C estimated
by both methods. Taking into account the cost and performance, Friedewald’s method is as good or even better for classifying
and managing patients. 相似文献
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Yazan M. Alsmadi Alaa M. Abdel-hamed Abo Eleyoun Ellissy Amged S. El-Wakeel Almoataz Y. Abdelaziz Vadim Utkin Ali Arshad Uppal 《Journal of The Franklin Institute》2019,356(8):4191-4214
This paper proposes an optimization scheme for optimal configuration and energy management of the micro-grid (MG), using the Cuckoo search optimization algorithm (CSOA). The selected MG supplies a load profile located between 30.119 latitude and 31.605 longitude. The energy produced by the MG generation sources, according to meteorological data of the proposed location, is calculated using MATLAB. The objective/fitness function is modeled and designed for minimizing the total investment cost (TIC) including capital, investment, operation and maintenance costs. A novel weighted goal attainment function (WGAF) has been proposed to reduce CO2 emissions and their associated costs. Moreover, WGAF also applies higher taxes on the amount of emissions that exceed governmental approved limits. To investigate the effects of WGAF on the TIC ($/year), annual cost of energy ($/kWh), and CO2 emissions, various weighted coefficients are analyzed. The simulation results have shown that the designed optimization scheme can robustly and efficiently produce the optimal MG configuration that is both eco-friendly and generates economic benefits. 相似文献
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Vibha Uppal Chittranjan Vij Gurdeep Kaur Bedi Anil Vij Basu Dev Banerjee 《Indian journal of clinical biochemistry : IJCB》2013,28(4):336-341
The study was planned to assess the prevalence of thyroid disorders in type 2 diabetes in North Indian population and to correlate the serum insulin and glycosylated haemoglobin levels with thyroid hormones. It is a case control study. One hundred and twenty patients of type 2 diabetes were included in the study along with 117 adults of the same age group and normal glucose levels as controls. All blood samples were taken from subjects who fasted for at least 12 h before the blood collection. Glycosylated hemoglobin was determined by ion exchange chromatography and serum insulin and thyroid hormones were assessed through enzyme linked immunosorbent assay. Fasting blood glucose and glycosylated haemoglobin levels were significantly higher in diabetics showing a poor glucose control. Serum tri-iodothyronine values were significantly lower in diabetics. There was a significant correlation between glycosylated haemoglobin and thyroid hormones. There was no correlation between serum insulin and thyroid hormones. 相似文献
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This two-part article considers certain fundamental symmetries of nature, namely the discrete symmetries of parity (P), charge conjugation (C) and time reversal (T), and their possible violation. Recent experimental results are discussed in some depth. In the first part of this article
we present a general background and discuss parity violation.
B Ananthanarayan is at the Centre for Theoretical Studies, Indian Institute of Science His research interests are in the field
of elementary particle physics.
J Meeraa is finishing her MSc at the Indian Institute of Technology, Chennai.
Bharti Sharma is finishing her MSc at Aligarh Muslim University.
Seema Sharma is finishing her MSc at the University of Rajasthan, Jaipur.
Ritesh K Singh is working towards his PhD at the Indian Institute of Science in the field of elementary particle physics. 相似文献
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Manisha Mavai Yogendra Raj Singh R. C. Gupta Sandeep K. Mathur Bharti Bhandari 《Indian journal of clinical biochemistry : IJCB》2018,33(2):222-228
Autonomic activity may be deranged in thyroid dysfunctions and may lead to cardiovascular morbidity and mortality. Myopathy is a common manifestation in thyroid disorders and may be associated with raised serum creatine kinase (CK). We hypothesized that cardiovascular abnormality in thyroid dysfunction may manifest as raised CK-MB. This study was designed to investigate the correlation of CK and its isoform CK-MB with thyroid profile and linear parameters of heart rate variability (HRV). The study was conducted on 35 hypothyroid and hyperthyroid patients each, and 25 age-matched healthy controls. Autonomic activity was assessed by simple short term 5-min HRV. Biochemical evaluation of serum thyroid profile, CK-NAC and CK-MB were estimated in all the subjects. Our results demonstrated low HRV in hypo- as well as hyperthyroid patients. We observed significantly higher serum CK levels in hypothyroid patients when compared to hyperthyroids and controls. However, no significant differences were observed in CK-MB levels in the three groups. Significant positive correlation of CK with TSH and negative correlation with some HRV parameters (LF power, HF power, total power, SDNN, RMSSD) was observed in hypothyroid patients. Whereas correlation of CK-MB with thyroid profile as well as HRV parameters was non-significant in all the groups. Based on the CK and CK-MB findings and their correlation, we conclude that the cardiovascular changes seen in thyroid dysfunctions may primarily be due to autonomic imbalance without apparent cardiac muscle involvement. Whereas, raised CK levels indicate predominantly skeletal muscle involvement in hypothyroid patients. 相似文献
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