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Induction of diabetes by Streptozotocin in rats 总被引:1,自引:0,他引:1
A. Akbarzadeh D. Norouzian M. R. Mehrabi Sh. Jamshidi A. Farhangi A. Allah Verdi S. M. A. Mofidian B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via
comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide
in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats,
makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus
in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic
Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal
species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin
was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic
and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine
volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities
were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas
tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin
decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta
cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction
of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose
increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and
C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that
the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas
swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal
metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose
increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. 相似文献
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Hadi Yaghoubinejad Nourollah Zarrinabadi Dariush Nejadansari 《Teachers and Teaching》2017,23(2):127-140
Research has shown that lack of motivation on either students or teachers’ part negatively influences language learning. The latter case is a serious problem in this regard, since an unmotivated teacher will extinguish his learners’ enthusiasm and energy for learning. This study employed a mixed-method approach to investigate the demotivating factors for Iranian junior high school teachers. For the quantitative stage of the study, a questionnaire on demotivation was distributed among 105 participant teachers to find out the dominant demotivating factors. In the qualitative stage, and in order to gain a better picture of the salient demotivating factors and the reasons behind them, a set of semi-structured interviews with 10 volunteer teachers were conducted. The results indicated that lack of social recognition and respect, few adequate rewards, lack of supports or understanding regarding English education, and a large number of students in a single English class were the major demotivating factors for Iranian teachers. In the interviews, it was found that the participants perceived the first two items as specific to Iranian educational context. Finally, the implications for policy-makers and material developers and suggestions for future researchers are discussed. 相似文献
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Ali Farhangi Dariush Norouzian Mohammad Reza Mehrabi Mohsen Chiani Zahra Saffari Maryam Farahnak Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(4):406-417
The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250–300 g (75–90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2–4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250–300 g (75–90 days). Transplantation was performed in rats after 2–4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation. 相似文献
4.
Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mohammad Reza Mehrabi Shirin Jamshidi Davood Zare Morvarid Shafiei 《Indian journal of clinical biochemistry : IJCB》2008,23(1):57-61
Flow cytometry has been employed as a method to study homogeneity of isolated islet subpopulations. After collagenase digestion
of rat pancreas and elutriation of tissue fragments, islets were isolated and dissociated, and cells were analyzed and sorted
according to their low forward angle light scattering properties by using automated flow cytometry. A standardized procedure
was developed for the preparation of rat islet cell grafts for purification of islet cells. In this process, after collagenase
digestion of pancreas, islets were isolated, dissociated, identification by dithizone method and then with enzymatic procedure
by DNase and trypsin, the islet cells changed into single cells and beta cells were identified by immunofluorescence method
and then assayed by flow cytometry. Methods have been developed for the preparation of suspension of viable rat pancreatic
islet cells and their analysis and sorting in the fluorescence activated cell sorter (FACC IV, Becton Dickinson, Sunnyvale,
Ca). Flow cytometry of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles
were lost during digestion. Purified endocrine islet cell grafts were prepared by pure beta-cells, without endocrine non-beta
cells. The purified aggregates were devoid of endocrine non-beta cells and damaged cells. 相似文献
5.
Simin Sohrabi Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mehri Mortazavi Mohammad Reza Mehrabi Mohsen Chiani Zahra Saffari Soheil Ghassemi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):354-359
The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification
of rabbit’s polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant
factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases
like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood
plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease
like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors
and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful.
The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify
factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was
separated from the blood sample, purified and used to identify factor VIII in the research. 相似文献
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