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Pushpa Durgawale P. S. Shukla Sonali Mishra 《Indian journal of clinical biochemistry : IJCB》1999,14(2):241-244
An unexpected increase in erythrocyte osmotic resistance during viral hepatitis in two patients prompted study of effects
of hepatitis (in Karad) on osmotic resistance. The test was performed by placing erythrocytes in saline solutions of decreasing
osmolarity and osmotic fragility defined in terms of the saline concentration at which hemolysis begins. Study included 44
infected subjects (acute viral hepatitis non-A non-B). All forty four infected subjects showed abnormal results when compared
to normal subjects. increased erythrocyte osmotic resistance i.e. hemolysis begins between 0.45% to 0.40% of NaCl and is completed
between 0.25% to 0.20% NaCl, whereas in normal subjects. hemolysis begins between 0.50% to 0.45% NaCl and is completed between
0.35% to 0.30% NaCl. Osmotic resistance was observed with increase in total bilirubin (mean±SD) (4.6 ±3.6), direct bilirubin
(3.0±2.5), SGOT (58.8±55.5) SGPT (114.2±150.3) activity. Physiologic shifts in erythrocyte osmotic resistance may be due to
changes in membrane lipid ratio. 相似文献
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Chavan VU Durgawale PP Sayyed AK Sontakke AV Attar NR Patel SB Patil SR Nilakhe SD 《Indian journal of clinical biochemistry : IJCB》2011,26(3):283-289
Twenty-four hour urinary albumin excretion (UAE) is considered as gold standard method for albuminuria measurement, but collection
of 24-h urine is inconvenient. The aim of present study was to evaluate whether albumin: creatinine ratio (ACR) and urinary
albumin concentration (UAC) in different spot urine samples correlate or not with 24-h UAE for screening of microalbuminuria
in type 2 diabetic patients. We collected first morning void (FMV), random urine sample (RUS) and 24-h urine, separately on
consecutive days from 104 type 2 diabetic patients. ACR and UAC in each spot urine sample compared with 24-h UAE with regard
to Pearson correlation coefficient. Pearson’s correlation of albumin: creatinine ratio (ACR) with 24-h UAE was (r = 0.802 and 0.623) in first morning void (FMV) and random urine sample (RUS), respectively. Pearson’s correlation coefficient
of urinary albumin concentration (UAC) compared with 24-h UAE was (r = 0.943 and 0.920), in FMV and RUS, respectively, P < 0.01. Results revealed that values in first morning void (FMV) were better correlated with 24-h urinary albumin excretion
(UAE), than the values in random urine sample (RUS). We conclude that the first morning void (FMV) may be able to replace
24-h urine collection, preferably urinary albumin concentration (UAC) in the initial screening of microalbuminuria in diabetic
patients. 相似文献
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Pushpa P. Durgawale P. S. Shukla Shubhangi D. Sontakke P. G. Chougule 《Indian journal of clinical biochemistry : IJCB》2001,16(1):110-112
In the present study the property of lectin agglutination in blood on normal and different cancer patients has been observed.
The purifiedSynadenium grantii root lectin was non blood group specific and its utility as a diagnostic tool in malignancy was studied. Hemagglutination
(units/ml) of red blood cells of different types of cancer was compared with the normal control's red blood cells. Out of
113 total cancer patients, only a group of 29 breast cancer patients showed significant increase in titre value (P<0.05) compared
to normal control. 相似文献
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Pushpa Durgawale Sudhir Kanase Pramod S. Shukla Shubhangi Sontakke 《Indian journal of clinical biochemistry : IJCB》2005,20(2):174-177
Cerebrospinal fluid protein estimation is a routine diagnostic tool for various diseases. The proposed modified bromocresol
green (BCG) method is applicable to automated as well as manual measurements. Protein concentrations and absorbance at 600
nm are linearly related throughout a wide range of concentrations from 10 mg % to 2000 mg %. Ninety C.S.F. samples were analyzed
and results obtained by both the modified BCG method and routine pyrogallol red method were compared. An excellent co-relation
was observed (n=90, g=0.996, P<0.001). 相似文献
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Pushpa Durgawale Sangita Patil P. S. Shukla Ajit Sontakke Satish Kakade Sunil Yadav 《Indian journal of clinical biochemistry : IJCB》2009,24(1):30-35
Fasting samples of 914 subjects from healthy population were analyzed for total cholesterol, triglyceride and three major
fractions of lipoproteins i.e. high-density lipoprotein cholesterol, low lipoprotein cholesterol and very low-density lipoprotein
cholesterol. The values obtained were (in mg/dl) 165.7±30.2,88.36±31.2, 44.86±10.68, 101.66±29.8 and 18.11±7.35 respectively.
When these subjects were grouped according to the age and sex, no appropriate differences were observed between most of the
groups. Triglycerides were found to be low and HDL cholesterol was high in female when compared with male of similar age.
Beyond age 40 years cholesterol level and low density lipoprotein cholesterol was found to be gradually increased in case
of women. Minor difference was observed with dietary pattern. Present study suggests that clinical evaluation of patient should
be made on the basis of these reference values for Western Maharashtra population. 相似文献
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