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Ganesa Yogeeswaran Charles E. Jebaraj Venkataraman Sriram 《Indian journal of clinical biochemistry : IJCB》1999,14(2):220-228
A comparison of the absorbance, enzyme/substrate concentration, reaction efficiency and sensitivity has been made for enzyme-based
clinical chemistry assays, using a conventional colorimeter versus a strip-microwell reader, in order to establish the value
of ultra-microchemical procedure, with reaction volume 87 μl (light path length=0.25 cm). By utilizing commercial kits available
for the quantitation of serum glucose, cholesterol and triglycerides, it has been established that the micro method is highly
cost effective (9–30 fold), reproducible and sensitive. Comparison of blood drawn by a finger prick (capillary) and venipuncture
for normal and pathological specimens show reproducibility between different laboratory technologists and in reference with
the values reported by an accredited reference laboratory. Since the micro method uses very little serum, it is most suitable
for analyses of small smaples, from large population-based field trials. However, the assay range has to be titrated for each
commercial kit to establish the enzyme/substrate equivalence. 相似文献
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