Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients     

Harleen Mangat  Tajamul Hussain  C Sundaram  MP JS Anandaraj 《Indian journal of clinical biochemistry : IJCB》2002,17(2):74-79

Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on³⁵S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle.  相似文献   

“What Say these Young Ones”: Students’ Responses to Shakespeare—An Icon of Englishness     

Vessela Balinska-Ourdeva  Ingrid Johnston  Joyti Mangat  Brent McKeown 《Interchange》2013,44(3-4):333-347

Challenging the taken-for-granted status of canonical authors, especially Shakespeare, is difficult, but not impossible. This research offers a glimpse into the inferential processes of a group of grade ten students from diverse backgrounds who read unfamiliar passages from Shakespeare. The findings reveal a complex picture of meaning-making, involving a variety of factors that play role in the students’ reading comprehension. The most crucial insight is the finding that subjective interpretations seem to hinder independent understanding and appreciation of Shakespeare’s texts beyond the literal level. Our research, conducted in a large, urban, culturally-diverse high school in Western Canada, shows that students tend to rely on uncritically absorbed bits and pieces of background information or popular culture references, as well as overgeneralized understanding of previously read Shakespearean texts, to form opinions about the author, which often carve an interpretive lens, colouring the reconstruction of the characters’ experiences in subsequent encounters with his works. The study juxtaposes actual experiences of students reading Shakespeare to culturally endorsed attitudes informing curriculum decisions and policies, as well as prevalent teaching practices in the high school classroom. Finally, it asks if teachers need to look at personal connections not as a strategy that leads to an analytic engagement, but as a form of analytic engagement, affirming that critical reflection is a competence that requires careful nurturing and guidance.  相似文献   

Relative subcellular distribution of calcium activated neutral protease in skeletal muscle of duchenne muscular dystrophy patients     

Harleen Mangat  M. P. J. S. Anandaraj  A. Anjaneyuiu 《Indian journal of clinical biochemistry : IJCB》1994,9(1):26-30

Calcium—activated neutral proteases (CANP) were examined in the subcellular fractions of the skeletal muscle from Duchenne muscular dystrophy patients and healthy individuals. Both and m CANP were detected in subcellular fractions of skeletal muscle. An increase in the quantity of CANP in subcellular particles of DMD muscle was observed. A 33 fold rise in the concentration of calcium in nuclei of dystrophic muscle was noted followed by cytosol, myofibrils, microsomes and mitochondria.  相似文献