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Kawamoto R Miyagi O Ohashi J Fukashiro S 《Sports biomechanics / International Society of Biomechanics in Sports》2007,6(2):187-198
The aim of this study was to identify critical kinetic variables that lead to increased ball velocity during a side-foot passing kick in soccer. Seven experienced male soccer players and eight inexperienced players participated in the experiment. They were instructed to perform side-foot kicks along the ground with maximum effort with an eye on the target line. The joint angles, angular velocities, and torques of the kicking leg were determined based on the three-dimensional kinematic data. The mean ball speed of the experienced group (21.4 +/- 1.5 m/s) was significantly faster than that of the inexperienced group (16.0 +/- 1.0m/s; P < 0.001). The motions of the inexperienced players tended to be less dynamic than those of the experienced players. The most noticeable difference in the kinetics of the kick was found in the hip flexion torque throughout the back-swing phase until the leg-cocking phase. The mean peak value of the experienced group (168 +/- 20 N x or m) was significantly greater than that of the inexperienced group (94 +/- 17 N x or m; P < 0.001). To increase ball speed during a side-foot passing kick, the generation of hip-flexion torque during the earlier stage of kicking is critical. 相似文献
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Tsuyoshi NakamuraHiroshi Ohashi 《Research Policy》2012,41(4):770-779
This paper examines intra-plant diffusion of new technology in the Japanese steel industry. The introduction of the basic oxygen furnace (BOF) was the greatest breakthrough in steel refining in the last century. Using unique panel data, the paper estimates total factor productivity by technology type, and associates the estimates with intra-plant diffusion. The paper finds that intra-plant diffusion accounts for about a half of the industry productivity growth. Large plants are likely to adopt the new technology earlier, but retain the old technology longer, than their smaller counterparts. 相似文献
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Immunoassay is one of the important applications of microfluidic chips and many methodologies were reported for decreasing sample∕reagent volume, shortening assay time, and so on. Micro-enzyme-linked immunosorbent assay (micro-ELISA) is our method that utilizes packed microbeads in the microfluidic channel and the immunoreactions are induced on the beads surface. Due to the large surface-to-volume ratio and small analytical volume, excellent performances have been verified in assay time and sample∕reagent volume. In order to realize the micro-ELISA, one of the important processes is the immobilization of antibody on the beads surface. Previously, the immobilization process was performed in a macroscale tube by physisorption of antibody, and long time (2 h) and large amount of antibody (or high concentration) were required for the immobilization. In addition, the processes including the reaction and washing were laborious, and changing the analyte was not easy. In this research, we integrated the immobilization process into a microfluidic chip by applying the avidin-biotin surface chemistry. The integration enabled very fast (1 min) immobilization with very small amount of precious antibody consumption (100 ng) for one assay. Because the laborious immobilization process can be automatically performed on the microfluidic chip, ELISA method became very easy. On-demand immunoassay was also possible just by changing the antibodies without using large amount of precious antibodies. Finally, the analytical performance was investigated by measuring C-reactive protein and good performance (limit of detection <20 ng∕ml) was verified. 相似文献
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Emilie Weibull Shunsuke Matsui Manabu Sakai Helene Andersson Svahn Toshiro Ohashi 《Biomicrofluidics》2013,7(6)
Understanding biomolecular gradients and their role in biological processes is essential for fully comprehending the underlying mechanisms of cells in living tissue. Conventional in vitro gradient-generating methods are unpredictable and difficult to characterize, owing to temporal and spatial fluctuations. The field of microfluidics enables complex user-defined gradients to be generated based on a detailed understanding of fluidic behavior at the μm-scale. By using microfluidic gradients created by flow, it is possible to develop rapid and dynamic stepwise concentration gradients. However, cells exposed to stepwise gradients can be perturbed by signals from neighboring cells exposed to another concentration. Hence, there is a need for a device that generates a stepwise gradient at discrete and isolated locations. Here, we present a microfluidic device for generating a stepwise concentration gradient, which utilizes a microwell slide''s pre-defined compartmentalized structure to physically separate different reagent concentrations. The gradient was generated due to flow resistance in the microchannel configuration of the device, which was designed using hydraulic analogy and theoretically verified by computational fluidic dynamics simulations. The device had two reagent channels and two dilutant channels, leading to eight chambers, each containing 4 microwells. A dose-dependency assay was performed using bovine aortic endothelial cells treated with saponin. High reproducibility between experiments was confirmed by evaluating the number of living cells in a live-dead assay. Our device generates a fully mixed fluid profile using a simple microchannel configuration and could be used in various gradient studies, e.g., screening for cytostatics or antibiotics. 相似文献
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Purpose: The influence of attention on postural control and the relationship between attention and falling has been reported in previous studies. Although a dual-task procedure is commonly used to measure attentional demand, such procedures are affected by allocation policy, which is a mental strategy to divide attention between simultaneous tasks. Therefore, we examined the effectiveness of salivary α-amylase, which is a physiological method for measuring attentional demand during postural control. Method: Sixteen healthy participants performed a postural-control task using the Balance System, which is a device that can be calibrated to a specific stability level (“Level 1 = least stable” to “Level 8 = most stable”). Levels 1, 2, and 3 were used for this study. Dependent variables measured were overall stability index, which represents the variance of platform displacement in degrees from a horizontal plane; probe reaction time, which was measured using a sound stimulator and recorder; and salivary α-amylase, which was measured using a portable salivary amylase analyzer. Results: As stability level of the test task decreased, both stability index and probe reaction time significantly increased. In addition, we identified a positive moderate correlation between probe reaction time and salivary α-amylase. Conclusions: Our results suggest that salivary α-amylase and probe reaction time reflect the change in attentional demands during a postural-control task and that salivary α-amylase may be an effective tool for evaluating attentional demands during postural control because it is noninvasive and simple to perform. 相似文献
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