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1.
The proximal-to-distal segmental sequence has been identified in many sports activities, including baseball pitching and ball kicking. However, proximal-to-distal sequential muscle activity has not been identified. The aims of this study were to establish whether sequential muscle activity does occur and, if it does, to determine its functional role. We recorded surface electromyograms (EMGs) for 17 muscles from the upper extremity and abdomen during overarm throwing and detected the onset and peak times as indices of muscle activity. The following electromyographic properties were commonly identified in the participants. First, sequential muscle activity was observed from the scapular protractors to the shoulder horizontal flexors and from the shoulder horizontal flexors to the elbow extensor, but not from the elbow extensor to the wrist flexor or forearm pronator. Secondly, the external oblique contralateral to the throwing arm became activated before the ipsilateral external oblique. This sequence is considered to be very effective for the generation of high force and energy in the trunk. Thirdly, the ipsilateral external oblique began its activity almost at foot strike. Finally, the main activity of the rectus abdominis appeared just before the point of release.  相似文献   
2.
Oxygen and glucose supply is one of the important factors for the growth and viability of the cells in cultivation of tissues, e.g., spheroid, multilayered cells, and three-dimensional tissue construct. In this study, we used finite element methods to simulate the flow profile as well as oxygen and glucose supply to the multilayered cells in a microwell array chip for static and perfusion cultures. The simulation results indicated that oxygen supply is more crucial than glucose supply in both static and perfusion cultures, and that the oxygen supply through the wall of the perfusion culture chip is important in perfusion cultures. Glucose concentrations decline with time in static cultures, whereas they can be maintained at a constant level over time in perfusion cultures. The simulation of perfusion cultures indicated that the important parameters for glucose supply are the flow rate of the perfusion medium and the length of the cell culture chamber. In a perfusion culture chip made of oxygen-permeable materials, e.g., polydimethylsiloxane, oxygen is hardly supplied via the perfusion medium, but mainly supplied through the walls of the perfusion culture chip. The simulation of perfusion cultures indicated that the important parameters for oxygen supply are the thickness of the flow channel and the oxygen permeability of the walls of the channel, i.e., the type of material and the thickness of the wall.  相似文献   
3.
Extracellular matrix (ECM) proteins are required for cell culture. In this paper, we report the use of O(2) plasma bonding to fabricate a perfusion culture microchamber array chip with identical-size ECM spots in the isolated microchambers. The chip was fabricated by assembly of two poly(dimethylsiloxane) (PDMS) layers, a microfluidic network layer, and an ECM array layer, which were aligned and then bonded by O(2) plasma oxidation with protection of the ECM microarray with a physical mask made from PDMS. We successfully cultivated Chinese hamster ovary K1 cells in the microchambers with fibronectin. In the fibronectin microchambers, the cells adhered and extended after 12 h of static culture and then grew over the course of 1 d of perfusion culture.  相似文献   
4.
Ground reaction force and wire tensile force were measured during test throws by three hammer throwers: the Asian record holder, who had a personal best of 83.47 m at the time of the investigation, and two university athletes, with personal bests of 59.95 m and 46.30 m respectively. They were filmed using three high-speed video cameras (250 Hz). The displacements of the hammer head and the athletes' centres of mass were calculated using three-dimensional analysis procedures. The Asian record holder's centre of mass and the hammer head on the final two turns exhibited approximate conjunctions of the hammer high point and the thrower's low point and vice versa about the hammer's azimuth angle. It is conjectured that the reason why the thrower's movement is asynchronous with the hammer's movement by approximately half a turn is to accelerate the hammer head in a manner similar to the way that the amplitude of a pendulum increases when it is pulled upward by a string against the downward movement of the swinging weight.  相似文献   
5.
Direction of spin axis and spin rate of the pitched baseball   总被引:1,自引:0,他引:1  
In this study, we aimed to determine the direction of the spin axis and the spin rate of pitched baseballs and to estimate the associated aerodynamic forces. In addition, the effects of the spin axis direction and spin rate on the trajectory of a pitched baseball were evaluated. The trajectories of baseballs pitched by both a pitcher and a pitching machine were recorded using four synchronized video cameras (60 Hz) and were analyzed using direct linear transform (DLT) procedures. A polynomial function using the least squares method was used to derive the time-displacement relationship of the ball coordinates during flight for each pitch. The baseball was filmed immediately after ball release using a high-speed video camera (250 Hz), and the direction of the spin axis and the spin rate (omega) were calculated based on the positional changes of the marks on the ball. The lift coefficient was correlated closely with omegasinalpha (r = 0.860), where alpha is the angle between the spin axis and the pitching direction. The term omegasinalpha represents the vertical component of the velocity vector. The lift force, which is a result of the Magnus effect occurring because of the rotation of the ball, acts perpendicularly to the axis of rotation. The Magnus effect was found to be greatest when the angular and translational velocity vectors were perpendicular to each other, and the break of the pitched baseball became smaller as the angle between these vectors approached 0 degrees. Balls delivered from a pitching machine broke more than actual pitcher's balls. It is necessary to consider the differences when we use pitching machines in batting practice.  相似文献   
6.
Ground reaction force and wire tensile force were measured during test throws by three hammer throwers: the Asian record holder, who had a personal best of 83.47 m at the time of the investigation, and two university athletes, with personal bests of 59.95 m and 46.30 m respectively. They were filmed using three high-speed video cameras (250Hz). The displacements of the hammer head and the athletes' centres of mass were calculated using three-dimensional analysis procedures. The Asian record holder's centre of mass and the hammer head on the final two turns exhibited approximate conjunctions of the hammer high point and the thrower's low point and vice versa about the hammer's azimuth angle. It is conjectured that the reason why the thrower's movement is asynchronous with the hammer's movement by approximately half a turn is to accelerate the hammer head in a manner similar to the way that the amplitude of a pendulum increases when it is pulled upward by a string against the downward movement of the swinging weight.  相似文献   
7.
The aims of this study were to establish the temporal-spatial relationship between muscle activity and the smash stroke of skilled badminton players and to assess performance accuracy using the ellipse of constant distance. We recorded the surface electromyographic (EMG) activity of selected superficial muscles of the stroking arm and shoulder--flexor carpi ulnalis, extensor carpi radialis, triceps brachii (lateral head), biceps brachii and trapezius (upper)--during the badminton smash. In the first part of the study, we examined the characteristics of muscle function and performance accuracy of skilled and unskilled individuals during the badminton smash. Five well-trained badminton players and five students with no experience of badminton were asked to smash a shuttle as hard as they could towards a vertical square target 4 m away, repeating the stroke 30 times. In general, the skilled players showed a more constant time from peak electromyographic amplitude to impact. Immediately after impact, the electromyographic activity of the triceps brachii and flexor carpi radialis of the skilled players decreased; in the unskilled participants, however, it continued until well after impact. The area of the ellipse of constant distance and the off-target distance, which were used as indices of performance accuracy, were smaller for the skilled than for the unskilled participants. In the second part of the study, one skilled and one unskilled participant performed 100 trials a day for 6 days. The time from peak electromyographic amplitude to impact in the extensor carpi radialis and flexor carpi ulnalis was more variable in the unskilled than in the skilled participant even after 6 days of practice, but the proximal muscles of the unskilled participant had a similar pattern of activity to that of the skilled player. Thus, controlling the distal muscles appears to be important for achieving accurate performance of the smash in badminton.  相似文献   
8.
Strategy effects on word searching in the Japanese letter fluency test were investigated using the Near-infrared Spectroscopy (NIRS). Participants were given a Japanese letter fluency test and they were classified into two types of strategy users, based on analysis of their recorded verbal responses. One group, AIUEO-order strategy users, employed sequential word searching based upon the imagery of the AIUEO-table which they mastered when young, and the other group, semantic strategy users, searched for the next word mainly based on semantic relations. The blood-flow volumes (OxyHb) measured of the AIUEO-order strategy users were greater than for the semantic strategy users in the frontal parts of the brain, and the blood-flow was more active in the right than in the left part of the brain. These different activation patterns suggest that the NIRS might be useful new brain imaging technique for examination of psychological process in verbal behavior.  相似文献   
9.
This study re-evaluated the magnitude of hand propulsion (HP) in the pull and push phases of the front crawl stroke and investigated the association between the angular velocity of shoulder roll (ωSR) and hand propulsive lift (HPL). ωSR was computed in the plane normal to a forward direction for 16 skilled swimmers performing the front crawl stroke at a maximal sprinting pace. HP, hand propulsive drag (HPD) and HPL were determined by a dynamic pressure approach. HP and HPD in the pull phase were greater than in the push phase (P < 0.05) while HPL in the pull phase was similar to that in the push phase. Eleven swimmers out of the 16 swimmers had a significant within-swimmers correlation between ωSR and HPL in the push phase (P < 0.05). That is, HPL increased in the push phase as the ωSR of rolling back to the neutral position became faster. A swimmer should use more drag for hand propulsion in the pull phase and propulsion from drag and lift equally in the push phase. Based on the relationship between ωSR and HPL in the push phase, a possible stroke technique to enhance HPL using ωSR is discussed.  相似文献   
10.
Cell culture in microfluidic systems has primarily been conducted in devices comprised of polydimethylsiloxane (PDMS) or other elastomers. As polystyrene (PS) is the most characterized and commonly used substrate material for cell culture, microfluidic cell culture would ideally be conducted in PS-based microsystems that also enable tight control of perfusion and hydrodynamic conditions, which are especially important for culture of vascular cell types. Here, we report a simple method to prototype perfusable PS microfluidics for endothelial cell culture under flow that can be fabricated using standard lithography and wet laboratory equipment to enable stable perfusion at shear stresses up to 300 dyn/cm2 and pumping pressures up to 26 kPa for at least 100 h. This technique can also be extended to fabricate perfusable hybrid PS-PDMS microfluidics of which one application is for increased efficiency of viral transduction in non-adherent suspension cells by leveraging the high surface area to volume ratio of microfluidics and adhesion molecules that are optimized for PS substrates. These biologically compatible microfluidic devices can be made more accessible to biological-based laboratories through the outsourcing of lithography to various available microfluidic foundries.  相似文献   
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