排序方式： 共有21条查询结果，搜索用时 15 毫秒
1.
Yoshihiro Okamoto Tomoyo Mita Testuri Kodama Yoshimitsu Gotoh Naoya Fujita Takashi Fukui Toshiyuki Masuzawa 《Indian journal of clinical biochemistry : IJCB》2015,30(3):351-356
We measured serum soluble vascular endothelial growth factor receptor 1 (sVEGFR1) and receptor 2 (sVEGFR2) levels in healthy Japanese individuals in order to establish a reference value using a specific ELISA. Significant differences were observed in serum sVEGFR1 and sVEGFR2 levels between children and adults. To demonstrate the usefulness of the reference value for children, we measured serum sVEGFR1 and sVEGFR2 levels in children with diarrhea positive (D+) hemolytic uremic syndrome (HUS) as a preliminary study. Serum sVEGFR2 levels in children with HUS were markedly higher than those in healthy children from the onset of D + HUS. The reference value for healthy children in the present study will allow normal and pathological conditions to be discriminated from each other in future study 相似文献
2.
Oxygen and glucose supply is one of the important factors for the growth and viability of the cells in cultivation of tissues, e.g., spheroid, multilayered cells, and three-dimensional tissue construct. In this study, we used finite element methods to simulate the flow profile as well as oxygen and glucose supply to the multilayered cells in a microwell array chip for static and perfusion cultures. The simulation results indicated that oxygen supply is more crucial than glucose supply in both static and perfusion cultures, and that the oxygen supply through the wall of the perfusion culture chip is important in perfusion cultures. Glucose concentrations decline with time in static cultures, whereas they can be maintained at a constant level over time in perfusion cultures. The simulation of perfusion cultures indicated that the important parameters for glucose supply are the flow rate of the perfusion medium and the length of the cell culture chamber. In a perfusion culture chip made of oxygen-permeable materials, e.g., polydimethylsiloxane, oxygen is hardly supplied via the perfusion medium, but mainly supplied through the walls of the perfusion culture chip. The simulation of perfusion cultures indicated that the important parameters for oxygen supply are the thickness of the flow channel and the oxygen permeability of the walls of the channel, i.e., the type of material and the thickness of the wall. 相似文献
3.
Extracellular matrix (ECM) proteins are required for cell culture. In this paper, we report the use of O(2) plasma bonding to fabricate a perfusion culture microchamber array chip with identical-size ECM spots in the isolated microchambers. The chip was fabricated by assembly of two poly(dimethylsiloxane) (PDMS) layers, a microfluidic network layer, and an ECM array layer, which were aligned and then bonded by O(2) plasma oxidation with protection of the ECM microarray with a physical mask made from PDMS. We successfully cultivated Chinese hamster ovary K1 cells in the microchambers with fibronectin. In the fibronectin microchambers, the cells adhered and extended after 12 h of static culture and then grew over the course of 1 d of perfusion culture. 相似文献
4.
This is the second study of the series, and its aim was to investigate the influence of using the suspended aid on circle kinetics. In addition to the kinematic data recorded for part one (Fujihara & Gervais, 2012), the pommel reaction forces and the force applied from the suspended aid (aid reaction force) were analysed in relation to the motion of a whole-body mass centre. The hip joint moment was also computed by combining all segments in the lower extremities into a single rigid segment. Generally, the use of the aid changed the magnitude of the mass-centre trajectory or horizontal pommel reaction forces but not their patterns. The results also showed that the net hip joint moment was altered during circles with the aid. In summary, a suspended aid can be used as a progression for a variety of goals because it allows gymnasts to practise circles which would not be possible without the aid. When a suspended aid is used, however, practitioners should be aware of the possible kinetic alteration caused by the external force from the aid. 相似文献
5.
Fujihara T Gervais P 《Sports biomechanics / International Society of Biomechanics in Sports》2012,11(1):34-47
The aim of this study was to investigate the influence of a suspended aid on the reaction forces during a basic skill on pommel horse. Twenty gymnasts performed three sets of 10 circles with and without a suspended aid on a pommel horse under which two force plates were set. The results confirmed that the suspended aid could reduce the magnitude of the pommel reaction forces during circles while maintaining the general loading pattern. On the left hand, the average and peak forces were attenuated to 0.59 body weight (BW) and 0.85 BW from 0.76 BW and 1.13 BW, respectively. The right hand experienced slightly larger forces with no-aid trials, but the asymmetry between the hands decreased with the aid. Despite a relatively large variability, all gymnasts experienced smaller impact peak forces with the aid. A suspended aid is most commonly used for a beginner gymnast as an introduction to pommel horse exercises. However, this study confirmed that it can also be useful for all levels of gymnasts who would like to practice pommel horse exercises with reduced pommel reaction forces for a purpose such as a progression for learning a new skill, control of training volume, or rehabilitation. 相似文献
6.
7.
Toshiyuki Fujihara Pierre Gervais 《Sports biomechanics / International Society of Biomechanics in Sports》2013,12(1):34-47
The aim of this study was to investigate the influence of a suspended aid on the reaction forces during a basic skill on pommel horse. Twenty gymnasts performed three sets of 10 circles with and without a suspended aid on a pommel horse under which two force plates were set. The results confirmed that the suspended aid could reduce the magnitude of the pommel reaction forces during circles while maintaining the general loading pattern. On the left hand, the average and peak forces were attenuated to 0.59 body weight (BW) and 0.85 BW from 0.76 BW and 1.13 BW, respectively. The right hand experienced slightly larger forces with no-aid trials, but the asymmetry between the hands decreased with the aid. Despite a relatively large variability, all gymnasts experienced smaller impact peak forces with the aid. A suspended aid is most commonly used for a beginner gymnast as an introduction to pommel horse exercises. However, this study confirmed that it can also be useful for all levels of gymnasts who would like to practice pommel horse exercises with reduced pommel reaction forces for a purpose such as a progression for learning a new skill, control of training volume, or rehabilitation. 相似文献
8.
A qualitative investigation of trainees＇ adjustment in Japan：A case study of trainees from Indonesia
In Japan, there is a residence status known as “the trainee”. This is the status for residents who undertake “activities to learn and acquire the technology, skills, or knowledge at public or private organizations in Japan”. With the introduction of the Industrial Training Program in 1993, trainees have been permitted to extend their stay in Japan for education and employment purposes up to a maximum of three years. This study aims to investigate the adjustment styles of trainees and to consider the changes in their attitudes toward Japan in the course of this adjustment. Six male Indonesian trainees （mean age=23.83） were invited to a semi-structured interview. They were employed at the same factory, involved in the manufacture of car components, and were trainees in their first-, second-, and third-years. The interview mainly focused on the trainees＇ Japanese language skills, the changes in their images of Japan or Japanese people, the communication between them and their Japanese colleagues, and the difficulties they encountered during their stay in Japan. The results revealed that there were clear differences between the first, second, and third trainees. As the trainees＇ Japanese language skills improved, their communication with the Japanese colleagues increased. Subsequently, they developed more positive images of Japan and Japanese people and hoped to have more informal communication with Japanese people. 相似文献
9.
Toshiyuki Fujihara Takafumi Fuchimoto Pierre Gervais 《Sports biomechanics / International Society of Biomechanics in Sports》2013,12(1):22-38
The aim of this study was to analyse the principal mechanics of circles. Seventeen university male gymnasts performed circles on an instrumented pommel horse model that enabled the pommel reaction forces to be recorded at 1000 Hz with two force plates. The circles were also videotaped using two digital video cameras operating at 60 frames per second. During circles, the vertical component of the reaction forces peaked in the double-hand support phases. Changes in the velocity of the centre of mass corresponded to the change in the tangential components of horizontal reaction forces. The velocity of the centre of mass had its peaks in the single-hand support phases and its local minimums in the double-hand support phases. The velocity of the ankles and that of the centre of mass of the head and trunk were minimal in the single-hand support phases and were maximal in the double-hand support phases. These results suggest that the circles were composed of vertical movements and two kinds of rotations: rotation of the centre of mass and rotation of the body itself about the centre of mass. These two kinds of rotations, which influence the legs' rotational velocity, are phase dependent. To further our understanding of circles, research on circles in different orientations on the pommel horse will be beneficial. 相似文献
10.
In the third study of this series, we examined how circles with a suspended aid would vary depending of the gymnast's level of expertise. We analysed circles with the aid performed by three groups of eight gymnasts: expert, intermediate, and developing groups. The gymnasts for the expert and intermediate groups were selected from the previous studies based on judges' evaluation of their non-aid circles. The developing group of eight gymnasts, who were unable to perform circles on pommel horse without an aid, were added to this study and performed three sets of 10 circles on the pommel horse with the aid. Discriminative biomechanical variables identified in the previous two studies were used to compare circles with the aid among the three groups. As a result, the expert group demonstrated a larger pommel reaction force and a smaller aid reaction force than the other two groups, implying that they were less dependent on the aid. The smaller aid reaction force resulted in a smaller net hip joint moment. Furthermore, the expert group showed a shorter total duration and a greater shoulder excursion. In summary, the suspended aid can be used in a progressive manner depending on the gymnast's level of expertise. 相似文献