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We report a case of a diabetic, heterozygote with near normal hematology, marginally low level of hemoglobin A2(HbA2) having an increased level of hemoglobin F(HbF) that was pancellularly distributed among the red cells. BioRad DiaSTAT measurements
gave a high glycated hemoglobin A1c(HbA1c) of 31.5% and the BioRad Variant analyzer recorded an HbA1c value which was very
low, in discordance with the detected blood glucose levels. Flow cytometry and polymerization chain reaction (PCR) based studies
were carried out which revealed the case to be that of the common hereditary persistence of fetal hemoglobin (HPFH)-3, an
Asian Indian mutation. Fructosamine estimation and HbA1c by Boronate affinity chromatography were able to resolve the discordant
value detected and was able to confirm the diabetes status. The case would have been a diagnostic dilemma, if reported without
correlation. 相似文献
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Merin Iype Subramanya Upadhya Sharmila Upadhya Gopalakrishna Bhat 《Indian journal of clinical biochemistry : IJCB》2008,23(4):401-403
Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various
studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model
was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used
a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia
B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical
procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were
measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant
elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared
to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained
for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating
to renal failure in the field of research. 相似文献
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The study was conducted on 38 patients admitted into the intensive care unit with a provisional diagnosis of sepsis and 25 apparently healthy volunteers as controls. Serum procalcitonin (PCT) was assayed by an electrochemiluminescence method. Serum ischemia modified albumin (IMA), expressed as absorbance units was assayed by the albumin cobalt binding test. Patients with sepsis had significantly higher IMA levels (1.087 ± 0.786) as compared with those without sepsis (0.085 ± 0.234) with a p value <0.0001. The receiver operator characteristic (ROC) plot showed a sensitivity of 100 % and a specificity of 86.2 %. The area under the curve of the ROC plot was 0.917 with a p value of <0.0001. The higher levels of IMA serve to highlight the occurrence of ischemic damage which could be a prelude to poorer prognosis. The performance characteristics of IMA warrants its inclusion along with PCT as a parameter in the diagnosis of sepsis. 相似文献
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B. V. Rajarama Bhat 《Resonance》2010,15(7):595-603
This article is on the use of ‘invariants’ in mathematics illustrated with some simple examples. 相似文献
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G. Kashinath Mira Pal K. Ramachandran P. Bhat 《Indian journal of clinical biochemistry : IJCB》1994,9(2):87-90
Serum phenytoin levels were measured in grand mal epilepsy patients receiving diphenyl hydantoin. The drug levels were correlated with various biochemical parametres. A linear relationship between the levels of diphenyl hydantoin and creatinine was observed. This positive correlation coefficient was found to be statistically signifficant. This correlation may be related to a positive Jaffe’s reaction seen with the chromogen diphenyl hydantoin. 相似文献
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Kabekode V.S. Bhat 《Journal of The Franklin Institute》1981,312(1):89-93
In this paper we characterize a symmetrizability property using the theory of output sets. Employing the basic properties of symmetric matrices and an efficient algorithm for systematic generation of output sets, an algorithm for testing the symmetrizability of a matrix is presented and illustrated. 相似文献