Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L.and Oryza meyeriana L.     

严成其  钱凯先  薛刚平  吴忠长  陈跃磊  颜秋生  张雪琴  吴平 《浙江大学学报(A卷英文版)》2004,5(10)

Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice bfeeding program for the improvement of bacterial blight resistance.  相似文献   

抗CMV病毒外壳蛋白CP基因导入黄瓜的研究   总被引：3，自引：0，他引：3  

何铁海  应成波  钱剑锐  严成其  杨金水 《河南科技学院学报》2001,29(3):27-28

将CMV-CP基因经农杆菌感染介导转化黄瓜子叶,将CMV-CP基因导入黄瓜细胞,在含有卡那霉素和羧卡青霉素的MS培养基上诱导子叶分化,获得一批转化处理的黄瓜再生苗,分化率为23%左右,转基因黄瓜试管苗移栽在蔬菜种植土沙有机质为541的混合土中,其移栽成活率可达56%;委托省农科院品种引进中心进行转基因黄瓜再生植株对CMV的抗性接种试验结果表明7号株系为高抗,2、4号株系为中抗.  相似文献   

野生稻与栽培稻体细胞杂交第4代特性研究初报   总被引：1，自引：0，他引：1  

皇甫伟国  应成波  裘尧军  陈国  严成其  颜秋生 《河南科技学院学报》2001,29(3):12-14

通过对D3代体细胞杂交后代进行白叶枯病检测,获得67份高抗白叶枯病体细胞杂交材料,并对D4代体细胞杂交材料人工白叶枯病接种和稻瘟病重发病区的田间自然诱发发病及大田种植结果分析.结果表明有86.6%的植株表现为抗白叶枯病,有16个株系较抗稻瘟病,有5个株系还存在分离现象,分离率为7.5%,有10个株系丰产性较好.  相似文献   

Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.   总被引：2，自引：0，他引：2  

严成其  钱凯先  薛刚平  吴忠长  陈跃磊  颜秋生  张雪琴  吴平 《Journal of Zhejiang University. Science. B》2004,(10)

Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.  相似文献