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Dimov N Muñoz L Carot-Sans G Verhoeven ML Bula WP Kocer G Guerrero A Gardeniers HJ 《Biomicrofluidics》2011,5(3):34102-3410212
To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His(6)-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS). 相似文献
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Systematic characterization of degas-driven flow for poly(dimethylsiloxane) microfluidic devices 总被引：1，自引：0，他引：1
Degas-driven flow is a novel phenomenon used to propel fluids in poly(dimethylsiloxane) (PDMS)-based microfluidic devices without requiring any external power. This method takes advantage of the inherently high porosity and air solubility of PDMS by removing air molecules from the bulk PDMS before initiating the flow. The dynamics of degas-driven flow are dependent on the channel and device geometries and are highly sensitive to temporal parameters. These dependencies have not been fully characterized, hindering broad use of degas-driven flow as a microfluidic pumping mechanism. Here, we characterize, for the first time, the effect of various parameters on the dynamics of degas-driven flow, including channel geometry, PDMS thickness, PDMS exposure area, vacuum degassing time, and idle time at atmospheric pressure before loading. We investigate the effect of these parameters on flow velocity as well as channel fill time for the degas-driven flow process. Using our devices, we achieved reproducible flow with a standard deviation of less than 8% for flow velocity, as well as maximum flow rates of up to 3 nL∕s and mean flow rates of approximately 1-1.5 nL∕s. Parameters such as channel surface area and PDMS chip exposure area were found to have negligible impact on degas-driven flow dynamics, whereas channel cross-sectional area, degas time, PDMS thickness, and idle time were found to have a larger impact. In addition, we develop a physical model that can predict mean flow velocities within 6% of experimental values and can be used as a tool for future design of PDMS-based microfluidic devices that utilize degas-driven flow. 相似文献
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Research in Science Education - This paper discusses how multimodal creative inquiry might be conceptualised and implemented for children’s meaning-making in science. We consider... 相似文献
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Nikolay Geraskin Lyubomir Pironkov Vasily Glebov 《European Journal of Engineering Education》2017,42(5):561-569
This paper presents the results of the European educational projects CORONA and CORONA-II which are dedicated to preserving and further developing nuclear knowledge and competencies in the area of VVER-type nuclear power reactors technologies (Water-Water Energetic Reactor, WWER or VVER). The development of the European Center of Competence for VVER-technology is focused on master’s degree programmes. The specifics of a systematic approach to training in the area of VVER-type nuclear power reactors technologies are analysed. This paper discusses enhancement of the training opportunities of the European Center that have arisen from advances in methodology and distance education. With a special attention paid to the European Nuclear Education Network (ENEN), the possibilities of further development of the international cooperation between European countries and educational institutions are examined. 相似文献
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In this paper, we propose a novel framework for a scholarly journal – a token‐curated registry (TCR). This model originates in the field of blockchain and cryptoeconomics and is essentially a decentralized system where tokens (digital currency) are used to incentivize quality curation of information. TCR is an automated way to create lists of any kind where decisions (whether to include N or not) are made through voting that brings benefit or loss to voters. In an academic journal, TCR could act as a tool to introduce community‐driven decisions on papers to be published, thus encouraging more active participation of authors/reviewers in editorial policy and elaborating the idea of a journal as a club. TCR could also provide a novel solution to the problems of editorial bias and the lack of rewards/incentives for reviewers. In the paper, we discuss core principles of TCR, its technological and cultural foundations, and finally analyse the risks and challenges it could bring to scholarly publishing. 相似文献
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Movchun Vasily Lushkov Richard Pronkin Nikolay 《Education and Information Technologies》2021,26(3):2523-2537
Education and Information Technologies - The aim of the article is to study the prediction model of an individual learning style in e-learning systems to enhance the effectiveness of dentistry... 相似文献
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