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Arun Kumar B. S. Dhillon D. N. Rao Geetha Menon Hari Shankar L. K. Dhaliwal Maiti Leema Nomita Chandhiok Neeta Kumar Rohini Sehgal Suneeta Mittal Sumitu Sahdev Kandpal Shobha V. L. Jindal 《Indian journal of clinical biochemistry : IJCB》2012,27(4):405-409
Malondialdehyde (MDA) is widely used as oxidative stress biomarker in biomedical research. Plasma is stored in deep freezers generally till analysis. Effect of such storage on MDA values, which may be variable and prolong, was incidentally observed in the ongoing study which is to estimate oxidative stress with oral iron. Plasma from blood samples of pregnant women (20–30 years age) in third trimester of singleton pregnancy (n = 139), consuming oral iron tablets was stored at −20 °C with intention of MDA estimation, as soon as possible. However logistic problems led this storage for prolonged and variable period (1–708 days). When values of MDA estimated using “Ohkawa” 79 method and readings were plotted against time to check the temporal effect, it showed a hyperbolic curve. Standard deviation (SD) was lowest when samples were tested within 3 weeks time. The samples analyzed within 3 weeks had mean ± SD value of 31.59 ± 26.11 μmol/L, while 123.7 ± 93.97 and 366.5 ± 189.8 μmol/L for samples stored for 1–3 and 4 months to 1 year respectively. Mean ± SD were 539.9 ± 196.8 in the samples store for more than a year. Rate of change in values was also lowest (0.0433 μmol/L/day) in the samples tested within first 3 weeks, which rose to 1.2 μmol/L/day during 3 month’s storage. This rate peaked at storage of 120 days (1.87 μmol/L/day) and fell to 0.502 μmol/L/day in the second year of storage. It is concluded that at −20 °C, only 3 weeks of storage time should be considered valid for fairly acceptable stability in MDA values. 相似文献
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Cholesterol synthesis and its suppression by LDL was measured in freshly isolated leucocytes from patients with acute myocardial infarction and healthy controls. Cells incubated for different time intervals in lipoprotein-deficient serum exhibited increased cholesterol synthesis. The magnitude of this increase was far greater in leucocytes of hyperlipemic patients than in normolipemic patients. Addition of LDL to the incubation medium produced suppression of cholesterol synthesis. This reduction was less in hyperlipemic patients as compared to normolipemic patients. These observations may imply reduced suppression of HMG-CoA reductase activity with high endogenous cholesterol synthesis in patients suffering with acute myocardial infarction. 相似文献
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The cholesterol of high density lipoprotein (HDL) and its sub-fractions HDL2, HDL3, and apoprotein concentrations were monitored in fifty seven male alcoholic subjects. Thirty five of them had normal liver
enzymes and twenty two had abnormal liver enzymes. Subjects with abnormal liver enzymes had elevated cholesterol of high density
lipoprotein and its sub-fractions and also Apo AI, AII concentrations in plasma, whereas in the group of patients with normal
liver enzymes, the concentrations of lipids and apoproteins did not differ significantly from the control group.
The quantitation of HDL cholesterol and its sub-fractions along with Apo AI and AII can be used as indicators to characterise
the state of hepatic function. 相似文献
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Neeta Singh 《Indian journal of clinical biochemistry : IJCB》2007,22(2):6-16
Apoptosis a physiological mechanism that eliminates excessive, damaged or unwanted cells, is a highly regulated pathway important
for maintaining homeostasis in multicellular organisms. It can be initiated through various signals via the extrinsic pathway
which involves death receptors, or via the intrinsic pathway which is initiated by intracellular damage and involves the mitochondria
and release of cytochrome c from it to further activate caspases. The Bcl-2 family of proteins is situated upstream to the
irreversible damage of cellular constituents and is an important checkpoint in the fate of a cell. The pro-apoptotic members,
BH3 only members include BID, BAD and BIM. They directly or indirectly activate multidomain BAX/BAK that constitute the requisite
gateway to the intrinsic pathway which operates at the mitochondrial surface and endoplasmic reticulum. In contrast, antiapoptotic
members such as Bcl-2, Bcl-XL bind and sequester activation. Downstream of mitochondria, the apoptosome involvement is seen
to generate caspase activity. Post mitochondria regulation involves IAPs, and their inhibitors. The pathogenesis of several
diseases such as cancer, neurodegenerative disorders, autoimmune disorders, heart disease, infectious diseases including AIDS
is closely related to aberrant apoptosis. Consequently interest has emerged in employing various the rapeutic approaches such
as gene therapy, antisense therapy, recombinant biologicals, organic and combinatorial chemistry, to specifically target apoptosis
signaling pathways such as death receptors FAS/TRAIL, Bcl-2, p53, IAPs, SMAC and caspases, etc. and are now advancing from
preclinical to clinical phase. 相似文献
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Role of oxidative stress while controlling iron deficiency anemia during pregnancy - Indian scenario
Neeta Kumar Nomita Chandhiok Balwan S. Dhillon Pratik Kumar 《Indian journal of clinical biochemistry : IJCB》2009,24(1):5-14
Iron Deficiency anemia ranks 9th among 26 diseases with highest burden. Asia bears 71% of this global burden. Adverse maternal and birth outcome associated
with hemoglobin status renders the issue worth attention. Indian scenario has worsened over the period despite continuous
international and national efforts. This indicates some lacunae in the approach and strategies applied. Various reports state
that even with maximum effort to increase outreach and monitoring for adherence to Iron schedule, consumer’s compliance remains
abysmally low. Recent studies has pointed out biological basis of side effects (gastrointestinal complains and systemic events)
as raised oxidative stress for which iron is the key catalyst. Up till now the only target of research has been to raise hemoglobin
of pregnant women above 11gm/dl. With the reports of pregnancy specific morbidities i.e. hemorrhage and septicemia with low
hemoglobin, eclampsia, small for gestation age, gestational diabetes with higher ranges of hemoglobin, alarm is raised to
define optimum range. Use of oxidative stress as biochemical marker with different doses and schedules has been defined because
India lack information for its own population upon oxidative stress status when iron is supplemented as per current guidelines.
Studies done in India and abroad have defined that too much and too less, both may raise oxidative stress and studies of this
sort may provide biochemical scale for optimization. This review therefore has evaluated currently available Indian research
and reports to understand the need of future research area. Important findings from other countries have been incorporated
for comparison. 相似文献
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