The effect of extremely high glucose concentrations on 21 routine chemistry and thyroid Abbott assays: interference study     

Serap ?uhadar  Mehmet K?seo?lu  Yasemin ?inpolat  Güler Bu?dayc?  Murat Usta  Tuna Semerci 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2016,26(1):53-60

Introduction

Extremely high glucose concentrations have been shown to interfere with creatinine assays especially with Jaffe method in peritoneal dialysate. Because diabetes is the fastest growing chronic disease in the world, laboratories study with varying glucose concentrations. We investigated whether different levels of glucose spiked in serum interfere with 21 routine chemistry and thyroid assays at glucose concentrations between 17-51 mmol/L.

Materials and methods

Baseline (group I) serum pool with glucose concentration of 5.55 (5.44-5.61) mmol/L was prepared from patient sera. Spiking with 20% dextrose solution, sample groups were obtained with glucose concentrations: 17.09, 34.52, and 50.95 mmol/L (group II, III, IV, respectively). Total of 21 biochemistry analytes and thyroid tests were studied on Abbott c8000 and i2000sr with commercial reagents. Bias from baseline value was checked statistically and clinically.

Results

Creatinine increased significantly by 8.74%, 31.66%, 55.31% at groups II, III, IV, respectively with P values of < 0.001. At the median glucose concentration of 50.95 mmol/L, calcium, albumin, chloride and FT4 biased significantly clinically (-0.85%, 1.63%, 0.65%, 7.4% with P values 0.138, 0.214, 0.004, < 0.001, respectively). Remaining assays were free of interference.

Conclusion

Among the numerous biochemical parameters studied, only a few parameters are affected by dramatically increased glucose concentration. The creatinine measurements obtained in human sera with the Jaffe alkaline method at high glucose concentrations should be interpreted with caution. Other tests that were affected with extremely high glucose concentrations were calcium, albumin, chloride and FT4, hence results should be taken into consideration in patients with poor diabetic control.Key words: assay interference, glucose interference, preanalytical phase, creatinine, Jaffe kinetic assay, thyroid function tests  相似文献   

Sub-nanomolar detection of thrombin activity on a microfluidic chip     

Nam Cao Hoai Le  Murat Gel  Yonggang Zhu  Jian Wang  Helen Dacres  Alisha Anderson  Stephen C. Trowell 《Biomicrofluidics》2014,8(6)

Bioluminescence resonance energy transfer (BRET) is a form of Förster resonance energy transfer. BRET has been shown to support lower limits of detection than fluorescence resonance energy transfer (FRET) but, unlike FRET, has not been widely implemented on microfluidic devices for bioanalytical sensing. We recently reported a microscope-based microfluidic system for BRET-based biosensing, using a hybrid, high quantum-efficiency, form of BRET chemistry. This paper reports the first optical fiber-based system for BRET detection on a microfluidic chip, capable of quantifying photon emissions from the low quantum-efficiency BRET² system. We investigated the effects of varying core diameter and numerical aperture of optical fibers, as well as varying microfluidic channel design and measurement conditions. We optimized the set-up in order to maximize photon counts and minimize the response time. The optimized conditions supported measurement of thrombin activity, with a limit of detection of 20 pM, which is lower than the microscope-based system and more than 20 times lower than concentrations reported to occur in plasma clots.  相似文献   

Query expansion with terms selected using lexical cohesion analysis of documents     

Olga Vechtomova  Murat Karamuftuoglu 《Information processing & management》2007

We present new methods of query expansion using terms that form lexical cohesive links between the contexts of distinct query terms in documents (i.e., words surrounding the query terms in text). The link-forming terms (link-terms) and short snippets of text surrounding them are evaluated in both interactive and automatic query expansion (QE). We explore the effectiveness of snippets in providing context in interactive query expansion, compare query expansion from snippets vs. whole documents, and query expansion following snippet selection vs. full document relevance judgements. The evaluation, conducted on the HARD track data of TREC 2005, suggests that there are considerable advantages in using link-terms and their surrounding short text snippets in QE compared to terms selected from full-texts of documents.  相似文献   

Separation and enrichment of sodium-motile bacteria using cost-effective microfluidics     

Jyoti P. Gurung  Moein Navvab Kashani  Sanaz Agarwal  Gonzalo Peralta  Murat Gel  Matthew A. B. Baker 《Biomicrofluidics》2021,15(3)

Many motile bacteria are propelled by the rotation of flagellar filaments. This rotation is driven by a membrane protein known as the stator-complex, which drives the rotor of the bacterial flagellar motor. Torque generation is powered in most cases by proton transit through membrane protein complexes known as stators, with the next most common ionic power source being sodium. Sodium-powered stators can be studied through the use of synthetic chimeric stators that combine parts of sodium- and proton-powered stator proteins. The most well studied example is the use of the sodium-powered PomA-PotB chimeric stator unit in the naturally proton-powered Escherichia coli. Here we designed a fluidics system at low cost for rapid prototyping to separate motile and non-motile populations of bacteria while varying the ionic composition of the media and thus the sodium-motive force available to drive this chimeric flagellar motor. We measured separation efficiencies at varying ionic concentrations and confirmed using fluorescence that our device delivered eightfold enrichment of the motile proportion of a mixed population. Furthermore, our results showed that we could select bacteria from reservoirs where sodium was not initially present. Overall, this technique can be used to implement the selection of highly motile fractions from mixed liquid cultures, with applications in directed evolution to investigate the adaptation of motility in bacterial ecosystems.  相似文献   

We game on skyscrapers: the effects of an equity-informed game design workshop on students’ computational thinking skills and perceptions of computer science     

Çakır  Nur Akkuş  Çakır  Murat Perit  Lee  Frank J. 《Educational technology research and development : ETR & D》2021,69(5):2683-2703

Educational technology research and development - This paper presents a game-design workshop built around a digital art installation featuring video games displayed over a real-world skyscraper to...  相似文献   

Aligning Teaching to Learning: A 3-Year Study Examining the Embedding of Language and Argumentation into Elementary Science Classrooms     

Brian Hand  Lori A. Norton-Meier  Murat Gunel  Recai Akkus 《International Journal of Science and Mathematics Education》2016,14(5):847-863

How can classrooms become communities of inquiry that connect intellectually challenging science content with language-based activities (opportunities to talk, listen, read, and write) especially in settings with diverse populations? This question guided a 3-year mixed-methods research study using the Science Writing Heuristic (SWH) approach in cooperation with 2 universities, area education agencies, 6 school districts, 32 elementary teachers, and over 700 students each year. The participating teachers engaged in a yearly summer institute, planned units, implemented this curriculum in the classroom, and contributed to ongoing data collection and analysis. Findings demonstrate that critical embedded language opportunities contribute to an increase in student Iowa Tests of Basic Skills (ITBS) scores in science and language based on level of implementation particularly for elementary students who receive free and reduced lunch (an indicator of living at the poverty level).  相似文献   

TURKISH PRESERVICE ELEMENTARY TEACHERS’ SELF-EFFICACY BELIEFS REGARDING MATHEMATICS AND SCIENCE TEACHING     

Murat Bursal 《International Journal of Science and Mathematics Education》2010,8(4):649-666

This study investigated Turkish preservice, elementary teachers’ personal mathematics teaching efficacy (PMTE), and science teaching efficacy (PSTE) beliefs at the end of their teacher education program. A majority of the participants believed they were well prepared to teach both elementary mathematics and science, but their PSTE scores were significantly lower than their PMTE scores. However, a significant correlation was found between the PMTE and PSTE scores. No significant gender effect on PMTE and PSTE scores was observed, but unlike the results from other countries, Turkish female preservice elementary teachers were found to have slightly higher PMTE and PSTE scores than their male peers. High school major area was found to be a significant predictor of participants’ PMTE and PSTE scores. Participants with mathematics/science high school majors were found to have significantly higher PMTE and PSTE scores than those with other high school majors.  相似文献   

The Evolution of Leadership Research in Higher Education for Two Decades (1995-2014): A Bibliometric and Content Analysis     

Murat Esen  Mehmet Sukru Bellibas  Sedat Gumus 《International Journal of Leadership in Education》2020,23(3):259-273

ABSTRACT

The present study aims to examine the leadership research in higher education in order to shed light on its development during the last two decades by revealing the evolving trends in research on leadership in higher education, the most prominent scholars working on related research, the most popular topics in related research, and the countries in which the related studies are based. Bibliometric method was employed in the analysis of the original research and review papers published in five prominent higher education journals between 1995 and 2014: Higher Education, Research in Higher Education, Studies in Higher Education, The Journal of Higher Education and The Review of Higher Education. The results of this bibliometric analysis show that the majority of the related articles come from three countries: the U.S.A., the UK and Australia. It is also found that the leadership research in higher education is still very scarce and has not shown any meaningful increase during the last two decades. In addition, content analysis is used to provide more in-depth information about the topical focus, purpose and methodology of the selected articles. The results of content analyses are discussed in detail, and suggestions for the future research are provided.  相似文献   

The relationship of organizational corruption with organizational culture, attitude towards work and work ethics: a search on Turkish high school teachers     

Ali Balc?  Murat ?zdemir  ?i?dem Apayd?n  Fatmanur ?zen 《Asia Pacific Education Review》2012,13(1):137-146

The aim of this study is to analyse organizational corruption and to determine its level of relation to attitude towards work, work ethics and organizational culture. The data in study have been collected from 441 public high school teachers employed in the central districts of Ankara in the school year of 2008–2009. Data have been collected through ‘Scale for Organizational Corruption’, ‘Scale for Attitude towards Work’, ‘Scale for Work Ethics’ and ‘Scale for Organizational Culture’, all of which were developed by the researchers in this study. Correlation and regression analysis techniques have been used in analysing the data. It is concluded from the study that there is a significant, though at an average level, relation between organizational corruption, organizational culture and work ethics and that there is a negative significant relation, though at a low level, between organizational corruption and attitude towards work. It is also concluded that the variables for attitude towards work, work ethics and organizational culture explain 38% of the variation in organizational corruption.  相似文献   

论中国特色社会主义理论体系与中国模式的关系——纪念中国共产党成立90周年     

木拉提·巴海 《乌鲁木齐职业大学学报》2011,20(2):1-5

中国特色社会主义是改革开放新时期以来我们党继续推进马克思主义中国化的伟大历史性创造,是包含着中国特色社会主义理论体系和中国模式等理论的和实践的多样性的对立统一体。中国模式和中国特色社会主义理论体系是实践与认识的辩证关系,本质上表现为实践与理论两个方面的双向互动、良性运行、相辅相成的关系,二者统一于发展中国特色社会主义伟大事业中,统一于中国特色社会主义伟大旗帜上。  相似文献