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A size-selective cell sorting microfluidic device that utilizes optical force is developed. The device consists of a three-dimensional polydimethylsiloxane microstructure comprised of two crossed microchannels in a three-dimensional configuration. A line shaped focused laser beam is used for automatic size-selective cell sorting in a continuous flow environment. As yeast cells in an aqueous medium are fed continuously into a lower channel, the line shaped focused laser beam is applied (perpendicular to the direction of flow) at the junction of the two crossed channels. The scattering force of the laser beam was employed to push cells matching specific criteria upward from one channel to another. The force depends on the size of the cells, the laser power, and the fluid flow speed. The variation in size of yeast cells causes them to follow different routes at the intersection. For flow speeds below 30 μm∕s, all yeast cells larger than 3 μm were removed from the main stream. As a result, a high purity sample of small cells can be collected at the outlet of bottom channel. 相似文献
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本研究利用Incofish项目开发的可持续发展指标开展渔业资源评估应用研究.研究对象为金色小沙丁鱼(Sardinella aurita)和短体小沙丁鱼(Sardinella maderensis).数据来源于1990年-2005年间塞内加尔个体渔业渔获量及由DR FRIDTJOF NANSEN调查船在塞内加尔海域获得的渔获量.采用Froese(2004年)提出的三个可持续捕捞指标,为渔业资源量的开发状况作出诊断和评估.这些指标包括:渔获物中成鱼的百分比（以100%为最佳）、达到最适开捕体长鱼的百分比（以100%为最佳）和大型产卵鱼的百分比（以0%为最佳,体长大于最适开捕体长1.1倍的雌鱼即为大型产卵鱼）.分析结果表明:金色小沙丁鱼处于过度捕捞状态,幼鱼在其渔获物中占有优势地位,捕捞到的金色小沙丁鱼多数小于最适开捕体长,即生长型过度捕捞;短体小沙丁鱼处于充分开发利用状态.总体结果揭示,可持续捕捞指标能够成为替代或完善传统资源评估方法的有用工具.渔业相关的利益主体（包括公众）通过这些指标能够避免过度捕捞,从而更好地理解渔业管理. 相似文献
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