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1.
Linda Desbois Adrien Padirac Shohei Kaneda Anthony J. Genot Yannick Rondelez Didier Hober Dominique Collard Teruo Fujii 《Biomicrofluidics》2012,6(4)
Water-in-oil microdroplets offer microreactors for compartmentalized biochemical reactions with high throughput. Recently, the combination with a sol-gel switch ability, using agarose-in-oil microdroplets, has increased the range of possible applications, allowing for example the capture of amplicons in the gel phase for the preservation of monoclonality during a PCR reaction. Here, we report a new method for generating such agarose-in-oil microdroplets on a microfluidic device, with minimized inlet dead volume, on-chip cooling, and in situ monitoring of biochemical reactions within the gelified microbeads. We used a flow-focusing microchannel network and successfully generated agarose microdroplets at room temperature using the “push-pull” method. This method consists in pushing the oil continuous phase only, while suction is applied to the device outlet. The agarose phase present at the inlet is thus aspirated in the device, and segmented in microdroplets. The cooling system consists of two copper wires embedded in the microfluidic device. The transition from agarose microdroplets to microbeads provides additional stability and facilitated manipulation. We demonstrate the potential of this method by performing on-chip a temperature-triggered DNA isothermal amplification in agarose microbeads. Our device thus provides a new way to generate microbeads with high throughput and no dead volume for biochemical applications. 相似文献
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This case is about Major League Baseball and the Dominican Republic. We explore how Major League Baseball has used this Caribbean island as an inexpensive labor market and the social problems of the situation. Specifically, we examine Corporate Social Responsibility, the leadership of Sandy Alderson, ethics and the various stakeholders involved. We also look into possible reform alternatives while addressing the central question: What is in the best interest of the players? 相似文献
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Adrien Sedeaud Guillaume Saulière Laurie-Anne Marquet Scott Del Vecchio Avner Bar-Hen Jean-François Toussaint 《European Journal of Sport Science》2017,17(6):656-664
The aim of this study was to quantify the impact of selections and shared selections in the rugby union. Players’ names, positions, and number of selections were collected for all XV de France’s games (1906–2014). Every team’s percentage of renewal of workforce was calculated for backs and forwards. During the 1987–2014 period, all second row forwards (locks), halfbacks, and centres’ shared selections (number of times when two players have competed together) were recreated. The Best vs. Rest method was applied to these remodelled dyads. They were analysed and compared with surrounding teammates as well as opponents. Head coaches similarly change their workforce for upcoming matches after winning or losing (around 30%), but losing teams renew significantly more positions in their line-ups. The recreated halfbacks, locks, and centres reveal a common pattern. Whether victorious or not, the ‘renewed couples’ victory percentage will congregate towards the XV de France’s victory percentage. For all the best recreated couples, the cumulated number of selections for forwards’ is always higher than the ones part of less efficient teams: 231.3?±?80 vs. 212.9?±?91 selections for locks’ teammates (Effect sizes (ES) small, possibly positive, 54.8%). In best recreated couples, number 8’s are significantly more experienced than their counterparts in less efficient pairs (ES small, likely positive, 76.3%). The XV de France’s collective effectiveness relies on a balance between stability and workforce renewal, which allows the building of specific position interactions and builds on experimented forwards packs. Selections and shared selections are serious collective performance parameters associated with performance. 相似文献
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Adrien Sedeaud Andy Marc Julien Schipman Karine Schaal Mario Danial Marion Guillaume 《Journal of sports sciences》2013,31(12):1146-1154
In a context of morphological expansion of the general population, how do athletes follow such a pattern of anthropometric growth? Is there any relation to performance? Biometric data including mass, height, body mass index (BMI) and age were collected for 50,376 American athletes representing 249,336 annual performers playing in professional baseball, football, ice hockey and basketball. Distributions by mass in National Football League (NFL) players are described by periods. Field goals have been studied in relation to players’ height in the National Basketball Association (NBA). Between 1871 and 2011, athletes from the four sports have increased significantly in mass, height and BMI, following a multi-exponential function series. Consequently, biometric differences between athletes and the general population are increasing gradually. Changes in the mass distribution within the NFL show the emergence of a biometrical specificity in relation to the field position. At the professional level, performance remains structured around precise biometric values. In the NBA, a height-attractor at 201.3 ± 6.3 cm for the best scorers is invariant, regardless of the level of play. These results suggest that laws of growth and biometrics drive high-level sport and organise performance around the specific constraint of each field position. Discrepancies between some mass and height developments question the (disproportionate) large mass increase (relative to the height increase) during the 1980s and 1990s. 相似文献
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Andy Marc Adrien Sedeaud Marion Guillaume Melissa Rizk Julien Schipman Juliana Antero-Jacquemin 《Journal of sports sciences》2013,31(6):524-532
AbstractAs opposed to many other track-and-field events, marathon performances still improve. We choose to better describe the reasons for such a progression. The 100 best marathon runners archived from January 1990 to December 2011 for men and from January 1996 to December 2011 for women were analysed. We determined the impact of historical, demographic, physiological, seasonal and environmental factors. Performances in marathons improve at every level of performance (deciles). In 2011, 94% of the 100 best men athletes were African runners; among women athletes they were 52%. Morphological indicators (stature, body mass and Body Mass Index (BMI)) have decreased. We show a parabolic function between BMI and running speed. The seasonal distribution has two peaks, in spring (weeks 14 to 17) and autumn (weeks 41 to 44). During both periods, the average temperature of the host cities varies close to optimal value for long distance race. African men and women runners are increasingly dominating the marathon and pushing its record, through optimal eco-physiological conditions. 相似文献
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Kwon Kyong-Ah Ford Timothy G. Salvatore Alicia L. Randall Ken Jeon Lieny Malek-Lasater Adrien Ellis Natalie Kile Mia S. Horm Diane M. Kim Sun Geun Han Minkyung 《Early Childhood Education Journal》2022,50(1):169-171
Early Childhood Education Journal - The original version of this article unfortunately contains some typo in Table 1. 相似文献
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Barbara Combes Jo Hanisch Mary Carroll Hilary Hughes 《International Information and Library Review》2011,43(3):137-143
This paper reports on the preliminary research findings of the sub-study group of a larger undertaking, the Australian Learning and Teaching Council (ALTC) priority project Re-conceptualising and Re-positioning Australian Library and Information Science Education for the Twenty-first Century. It examines student experiences of library and information science (LIS) education across both the tertiary and vocational education sectors in Australia. The student sub-study group’s task was to provide the student/recent graduate perspective on LIS education in Australia. The research considered four major themes to find out how students and recent graduates felt about LIS education in Australia. The themes were: learning opportunities; learner attributes; learning experiences; and learner outcomes. In the second half of 2010, self-administered anonymous web questionnaires and focus groups were used to explore issues such as student demographics, socio-economic backgrounds, past experiences, expectations and rationale behind course choices. In addition the destinations of graduates were explored. Key findings suggest there are various pathways that students take to achieve a LIS qualification in Australia; students are generally optimistic about the future of the LIS profession, and consider that technology will continue to play a key role in future career options; they stress the importance of practical workplace experiences as part of course structure; their satisfaction levels with current courses are high and students feel confident they have been provided with the skills required to begin their professional life. 相似文献
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István Tóth-Király Beáta Bőthe Gábor Orosz Adrien Rigó 《Structural equation modeling》2018,25(2):267-286
This article assesses the multidimensionality of the Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS) using bifactor exploratory structural equation modeling (bifactor ESEM). The first study relies on a sample of community adults (N = 2,301), and revealed the superiority of a bifactor ESEM representation, supporting the 6-factor structure of BPNSFS ratings, and the presence of a single continuum of need fulfillment relative to 2 distinct dimensions reflecting need satisfaction and frustration. These results were replicated in a second representative sample of the Hungarian adult population (N = 504), as well as across gender, and found no evidence of differential item functioning as a function of age. Relative to males, females presented higher levels of relatedness satisfaction and lower levels of competence satisfaction. Finally, autonomy frustration decreased with age, whereas competence satisfaction and frustration presented opposite curvilinear tendencies, showing that the fulfillment of this need increased sharply for younger participants, a tendency that became less pronounced with age. 相似文献