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排序方式: 共有73条查询结果,搜索用时 31 毫秒
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2.
Ajay Kumar T. A. Venkita Subramanian Usha P. Sharma 《Indian journal of clinical biochemistry : IJCB》1992,7(2):165-168
Protein fraction termed P2 was obtained from sonicates of Mycobacterium smegmatis, subjected to ammonium sulphate precipitation. P2 fraction was further fractionated into 4 fractions (PeakI-IV) by DEAE cellulose ion-exchange chromatography. ELISA was performed
on the sera of 104 tuberculous cases and 62 controls, using P2 fractions Peak I as test antigens. Though the mean ELISA values in tuberculous cases were higher than the controls, no statistically
significant difference was found between the two. Antigens P2, Peak I and IV were tested on Western blots with pooled sera from tuberculous cases as well as controls. P2 fraction, Peak-I and IV were separated on PAGE-SDS, electroblotted onto nitrocellulose Sheets and the blots were subjected
to ELISA. Peak IV appeared as a single band (M.W. 55,000). P2 fraction exhibited some discriminatory bands on development of blots. 相似文献
3.
K. Pratibha Usha Anand Rajni Agarwal 《Indian journal of clinical biochemistry : IJCB》2004,19(2):128-131
Serum adenosine deaminase (ADA), 5′ nucleotidase (5′NT) and malondialdehyde (MDA) were estimated in patients with acute infective
hepatitis (AIH) along with the routine parameters of liver disease. Present study is done to evaluate these special parameters
in patients with clinical history of AIH and to assess the utility of these parameters as diagnostic/ prognostic indices of
liver function and to correlate special parameters with routine live function tests (LFT). ADA, 5′NT and MDA along with routine
LFT was estimated in 25 patients with AIH and 25 samples from healthy voluntary blood donars served as the control group.
Routine LFT was estimated by standard clinical chemistry procedures on dade behring analyser and ADA, 5′NT and MDA were estimated
by berthlot reaction, fiske and subbarao method and thiobarbituric acid method respectively.
Statistical analysis showed that serum ADA, 5′NT and MDA were significantly higher in patients as compared with the controls.
There was a significant positive correlation between ADA and total bilirubin and MDA and total bilirubin. Hence we can conclude
that these tests would be more sensitive to diagnose the patients with AIH and that the raised bilirubin levels could be looked
upon, as a protective mechanism which the liver has evolved in order to combat oxidative stress. 相似文献
4.
Learning about Spelling Sequences: The Role of Onsets and Rimes in Analogies in Reading 总被引:2,自引:0,他引:2
Usha Goswami 《Child development》1991,62(5):1110-1123
Children's phonological awareness, especially their awareness of rhyme, is known to be importnat for later reading development. However, the exact nature of the connection between phonological skills and reading is not yet known. One possibility is that when children analyze written words in reading, they find it relatively easy to learn about spelling sequences within these words that reflect phonological categorizations such as rhyme. This hypothesis implies that learning to read words is not a purely visual process. For example, spelling sequences that reflect the intrasyllabic linguistic units of onset (initial consonants) and rime (vowel and final consonants), and thus are connected to rhyme, may be easier to learn about than other spelling sequences. In Experiment 1, children learned more about shared consonant blends at the beginnings of words ( tr im- tr ap), which constituted the onset, than at the ends of words (wi nk -ta nk ), which broke up the rime. In contrast, Experiment 2 showed that when words shared a vowel as well as a consonant blend, more was learned about spelling sequences at the ends of words (w ink -p ink ), which now reflected the rime, than at the beginnings of words ( tr im- tr ip), where the vowel extended the onset. So intrasyllabic phonological knowledge does seem to play a role in learning about spelling sequences in reading. 相似文献
5.
Madhusudhana Rao A Anand U Anand CV 《Indian journal of clinical biochemistry : IJCB》2011,26(1):28-31
Numerous lines of evidence implicate a role of myeloperoxidase (MPO) in the pathogenesis of cardiovascular disease (CVD).
It is a well accepted fact that patients with chronic kidney disease (CKD) are at an increased risk for CVD. MPO is a pro-oxidant
enzyme which could be involved in the increased susceptibility of these patients to CVD. Hence, the levels of plasma MPO was
determined in healthy controls as well as in patients with CKD [stratified with the level of their kidney failure as CKD stages
II–V (end stage renal disease)]. Plasma MPO was assayed by a spectrophotometric method. Serum urea and creatinine were estimated
on a clinical chemistry analyzer using standard laboratory procedures. The mean plasma MPO levels were significantly lower
with advancing stages of renal failure (P < 0.001). There was a positive correlation between MPO and GFR (r = +0.89, P < 0.001) and a negative correlation with urea (r = −0.85, P < 0.001) and creatinine (r = −0.82, P < 0.001). While an inverse association was observed between plasma MPO and urea in CKD patients, such an association was
not observed in control subjects (P = 0.43). In conclusion, the decline in plasma MPO levels may be due to the inhibitory effect of uraemic toxins on the enzyme. 相似文献
6.
M. Swarna M. Sujatha P. Usha Rani P. P. Reddy 《Indian journal of clinical biochemistry : IJCB》2004,19(2):163-167
Recent studies have presented evidence for the involvement of L1CAM gene mutations in various X-linked mental retardation
syndromes. The neural cell adhesion molecule, L1CAM is a transmembrane protein belonging to the super family of the immunoglobulins
that play a key role in embryonic development of the nervous system and is involved in memory and learning. No studies were
carried out from India on L1 CAM gene in X-linked mental retardation syndromes. Hence, an investigation was taken up to delineate
the role of L1CAM gene in mental retardation.
Two families (Family I and Family II) having only two members affected with mental retardation in each family were studied
for mutations in L1CAM gene. In family II, the younger sibling showed deletion involving region between the nucleotide 13,773
(intron 25) and 14,158 (intron 27) region. The mutation what we observed in younger sibling of the family II is a novel mutation
which was not hitherto reported in the world literature. 相似文献
7.
Poonam Gautam Taruna Madan W. N. Gade P. Usha Sarma 《Indian journal of clinical biochemistry : IJCB》2006,21(2):12-19
Allergenic/antigenic proteins are known to induce Type I and Type III hypersensitivity reactions leading to allergic bronchopulmonary
aspergillosis (ABPA) in immunocompetent host. The common structural features or intrinsic properties of the allergens/antigens
leading to allergenicity in a host are not well understood. In the current report, comparative analysis of proteins on two
dimensional gel electrophoresis (2D-3) and specific IgE immunoblots ofA. fumigatus secretory proteins (1st, 2nd and 3rd week culture filtrate proteins) was carried out. We observed a total of 159 proteins in 1st, 2nd and 3rd week culture filtrates ofA. fumigatus. Specific IgE immunoreactivity was observed in 75 proteins with different intensity. Third week culture filtrate showed maximum
number of proteins, 142, and specific IgE immunoreactive proteins, 65. MALDI-TOF analysis resulted in putative identification
of two allergens as hypothetical protein YBL057c fromSaccharomyces cereviseae and unnamed protein product fromDebaryomyces hansenii (similar to IPF14568 ofCandida albicans). Identification of a repertoire of specific IgE immunoreactive proteins will facilitate the studies on structure-function
relationship of these proteins relevant for diagnosis and pathogenesis. 相似文献
8.
Usha Kotelawala 《International Journal of Science and Mathematics Education》2016,14(6):1113-1131
This report examines teachers’ self-espoused attitudes and beliefs on proving in the secondary mathematics classroom. Conclusions were based on a questionnaire of 78 US mathematics teachers who had completed at least 2 years of teaching mathematics at the secondary level. While these teachers placed importance on proving as a general mathematical skill, when they discuss their own classrooms, procedural skill consistently surpasses proof-related activities in importance for a majority of high school teachers. Furthermore, teachers labeling their own past experiences in proving as causing anxiety are predictably more likely to put less value on proving. Interestingly, the quantity of past college mathematics courses is a reverse predictor indicating that further study should consider how students perceptions of proving change as they pass through a mathematics major. 相似文献
9.
It is well-documented that news media (both traditional and social) have a significant impact on intercultural relations in contemporary societies. This paper compares results of two surveys where we asked Australians about their news sources. The study shows that Australian news consumers from diverse cultural backgrounds are alienated from Australian mainstream news media, increasingly relying on online and social media sources for their information. The paper situates this empirical research in the contemporary articulation of uses and gratification theory to understand audiences’ preferred media sources for news and information and the potential implications for intercultural relations in Australia. 相似文献
10.
Educational Neuroscience: Defining a New Discipline for the Study of Mental Representations 总被引:1,自引:0,他引:1
ABSTRACT— Is educational neuroscience a “bridge too far”? Here, we argue against this negative assessment. We suggest that one major reason for skepticism within the educational community has been the inadequate definition of the potential role and use of neuroscience research in education. Here, we offer a provisional definition for the emerging discipline of educational neuroscience as the study of the development of mental representations. We define mental representations in terms of neural activity in the brain. We argue that there is a fundamental difference between doing educational neuroscience and using neuroscience research results to inform education. While current neuroscience research results do not translate into direct classroom applications, educational neuroscience can expand our knowledge about learning, for example, by tracking the normative development of mental representations. We illustrate this briefly via mathematical educational neuroscience. Current capabilities and limitations of neuroscience research methods are also considered. 相似文献