排序方式： 共有8条查询结果，搜索用时 15 毫秒
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Chun Pang Yu-chen Sheng Ping Jiang Hai Wei Li-li Ji 《Journal of Zhejiang University. Science. B》2015,16(7):602-610
Chlorogenic acid (CGA), a polyphenolic compound, is abundant in fruits, dietary vegetables, and some medicinal herbs. This study investigated the prevention of CGA against acetaminophen (AP)-induced hepatotoxicity and its engaged mechanisms. CGA reversed the decreased cell viability induced by AP in L-02 cells in vitro. In addition, CGA reduced the AP-induced increased serum levels of alanine/aspartate aminotransferase (ALT/AST) in vivo. The effect of CGA on cytochrome P450 (CYP) enzymatic (CYP2E1, CYP1A2, and CYP3A4) activities showed that CGA caused very little inhibition on CYP2E1 and CYP1A2 enzymatic activities, but not CYP3A4. The measurement of liver malondialdehyde (MDA), reactive oxygen species (ROS), and glutathione (GSH) levels showed that CGA prevented AP-induced liver oxidative stress injury. Further, CGA increased the AP-induced decreased mRNA expression of peroxiredoxin (Prx) 1, 2, 3, 5, 6, epoxide hydrolase (Ephx) 2, and polymerase (RNA) II (DNA directed) polypeptide K (Polr2k), and nuclear factor erythroid-2-related factor 2 (Nrf2). In summary, CGA ameliorates the AP-induced liver injury probably by slightly inhibiting CYP2E1 and CYP1A2 enzymatic properties. In addition, cellular important antioxidant signals such as Prx1, 2, 3, 5, 6, Ephx2, Polr2k, and Nrf2 also contributed to the protection of CGA against AP-induced oxidative stress injury. 相似文献
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国民党和青年党，是两个在中国政坛上有重大影响的政党。抗战前的十余年间，双方曾有过几次合作的努力并最终走到了一起，但在这一过程中，矛盾和斗争是贯穿始终的。这既是国民党长期实行“一党专政”造成的，也是青年党追求自身政治利益的结果。 相似文献
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由于单核苷酸多态性(SNPs)在寻找致病基因，了解遗传多样性、环境与基因的相互作用方面有重大价值，SNPs研究已越来越多地引起了学术界和产业界的兴趣．CYP1A1编码的酶在烟草的主要几类前致癌物(如PAHs和芳香胺类物质)的代谢活化中有重要作用，其多态性与个体对环境相关的癌症的易感性密切相关．本研究采用PCR-RFLP技术在上海人群中，对CYP1Al的两个SNPs：CYP1A1 m1和m2进行了基因分型．结合其它已发表的数据，我们的研究表明，在中国人群的不同群体中，m1等位基因的频率分布比较一致，而m2等位基因的频率分布则存在着显著的不同． 相似文献
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Hai-ping LU Martin EDWARDS Qi-zhao WANG Hai-jun ZHAO Hao-wei FU Jian-zhong HUANG Angharad GATEHOUSE Qing-yao SHU 《Journal of Zhejiang University. Science. B》2015,16(2):113-122
目的:分析CYP81A6基因在苯达松及甲磺隆处理下的诱导表达模式,解释该基因与两种除草剂代谢相关的可能原因。创新点:从两种除草剂降解途径中产生的小分子物质的结构相似性出发,通过基因诱导表达的特点分析,解释CYP81A6和两种除草剂降解相关的原因。方法:通过实时定量聚合酶链反应(PCR)来分析基因表达的特点;利用CYP81A6启动子与GUS报告基因构建的载体来分析组织特异性表达;通过亚细胞定位来确定CYP81A6发挥功能的场所。结论:CYP81A6基因受苯达松及甲磺隆诱导,在不同的时间点开始上调,说明了甲磺隆的降解中间产物可以诱导这个基因的表达;CYP81A6是组成型表达,在根、茎、叶中均有表达;亚细胞定位结果证明CYP81A6是一个内质网上的蛋白。 相似文献
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目的：观察有氧运动对大鼠肝脏功能以及CYP7A1mRNA的表达。方法：对高脂饮食饲养的肥胖及肥胖抵抗大鼠进行8周的运动干预，用组织学染色及血清生化检测，评价大鼠肝脏脂代谢及功能；用相对定量PCR评价CYP7A1mRNA的表达情况。结果：肥胖对照组大鼠HE染色肝细胞胞质内显现大小不等空泡，肝窦间隙明显变窄，肥胖游泳组肝细胞形态基本正常，细胞核居中，肝细胞脂肪变性明显减轻，肝窦间隙无明显变化；肥胖对照组、肥胖抵抗对照组大鼠血清ALT、AST水平高于普饲对照组并且具有非常显著的差异，肥胖游泳组大鼠血清ALT、AST显著低于肥胖对照组；肥胖对照组大鼠CYP7A1mRNA相对表达量高度显著低于普饲对照组。肥胖游泳组大鼠CYP7A1mRNA相对表达量显著高于肥胖对照组。结论：10周高脂饮食可引肥胖及肥胖抵抗大鼠血脂出现异常，脂质代谢紊乱、肝脏出现脂肪沉积、肝功能异常；有氧运动可能通过增加肥胖大鼠肝脏CYP7A1表达，改善胆固醇转化障碍，从而达到改善10周高脂饮食导致的肝功能异常的现象。 相似文献
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Ashavaid TF Raje HS Shah BV Shah SA 《Indian journal of clinical biochemistry : IJCB》2011,26(1):18-21
Single nucleotide polymorphisms in CYP3A5 (A6986G) and MDR-1 (C3435T) genes have been shown to be associated with the pharmacokinetics
of tacrolimus in case of renal transplant recipients. Knowing these genotypes of the recipients before undergoing transplantation,
is therefore essential for physicians to adjust the starting dose of tacrolimus in order to avoid drug induced nephrotoxicity.
We have designed an allele specific PCR method for easier and rapid detection of these polymorphisms. 20 Indian renal transplant
recipients on tacrolimus who developed nephrotoxicity within 1 month of transplantation and 58 Indian non-transplant subjects
having the risk factors for kidney disease i.e. hypertension or diabetes or the family history of these, have been studied
for these SNPs by allele specific PCR method. The data suggest that the heterozygosity of CYP3A5 and mutant allele frequency
of MDR-1 SNP is higher in transplant patients as well as in general population. 相似文献
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目的:研究穿心莲内酯对小鼠肝细胞色素P450的影响。方法:小鼠给药穿心莲内酯或3-甲基胆蒽后,分别测量小鼠肝脏内P450酶的总含量、CYP1A1/2的活性和蛋白表达等指标。结果:穿心莲单独给药时,对CYP1A1/2的活性及蛋白表达没有影响。穿心莲内酯和3-甲基胆蒽联合给药时对CYP1A1/2的活性呈诱导作用,同时CYP1A1/2的蛋白表达均显著高于空白对照。结论:穿心莲内酯与3-甲基胆蒽联合用药时对小鼠肝P450的活性及蛋白表达均显示出一定的调控作用。 相似文献
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细胞色素Ｐ４５０ ２Ｄ６９ＣＹＰ２Ｄ＾０基因２０６４Ｇ→Ａ突变,造成表达产物２１２位上谷氨酸变成甘氨酸,从而引起酶分子失活,被称为ＣＹ２Ｄ６＾＊６。利用等位基因特异扩增法,建立了ＡＳＡ－ＰＣＲ测定了ＣＹＰ２Ｄ６＾＊６的方法,经３９６例测定,说明本法快捷,准确,测定结果显示ＣＹＰ２Ｄ６＾＊６与ＣＹＰ２Ｄ６Ｔ共存,如何只为准确预测ＣＹＰ２Ｄ６酶活性,对ＣＹＰ２Ｄ６＾＊６进行测定并无意义。 相似文献
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