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1.

Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites

Ya-e Zhao Zheng-hang Wang Yang Xu Ji-ru Xu Wen-yan Liu Meng Wei Chu-ying Wang 《Journal of Zhejiang University. Science. B》2012,13(10):763-768

To our knowledge, few reports on Demodex studied at the molecular level are available at present. In this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (CHS) gene fragments of Demodex folliculorum, Demodex brevis, and Demodex canis (three isolates from each species) from Xi’an China, by designing specific primers based on the only partial sequence of the CHS gene of D. canis from Japan, retrieved from GenBank. Results show that amplification was successful only in three D. canis isolates and one D. brevis isolate out of the nine Demodex isolates. The obtained fragments were sequenced to be 339 bp for D. canis and 338 bp for D. brevis. The CHS gene sequence similarities between the three Xi’an D. canis isolates and one Japanese D. canis isolate ranged from 99.7% to 100.0%, and those between four D. canis isolates and one D. brevis isolate were 99.1%–99.4%. Phylogenetic trees based on maximum parsimony (MP) and maximum likelihood (ML) methods shared the same clusters, according with the traditional classification. Two open reading frames (ORFs) were identified in each CHS gene sequenced, and their corresponding amino acid sequences were located at the catalytic domain. The relatively conserved sequences could be deduced to be a CHS class A gene, which is associated with chitin synthesis in the integument of Demodex mites. 相似文献

2.

蚕蛹壳聚糖制取工艺优化研究

冯丹朱新鹏何小兰方琼《安康学院学报》2011,23(1):86-88

通过正交试验,对蚕蛹甲壳素脱乙酰制备壳聚糖的条件进行了优化研究。结果表明：NaOH浓度对蚕蛹甲壳素脱乙酰的影响最大,其次为处理温度,处理时间和固液比影响较小。优化后的工艺条件为：NaOH浓度50%、处理温度90℃、时间10h、固液比1：15。经验证试验,在该条件下,得到的蚕蛹壳聚糖脱乙酰度可达到72%。相似文献

3.

甲壳素、壳聚糖在饲料工业上的应用 总被引：3，自引：0，他引：3

朱斌《四川职业技术学院学报》2005,15(4):103-105

本文从甲壳素、壳聚糖对动物的生理作用和它们在饲料工业上的初步应用从两个方面进行了讨论,由此说明,随着对甲壳素、壳聚糖在饲料工业中应用的深入研究,必将对养殖业发展产生深远的影响相似文献

4.

壳聚糖硫酸盐的制备及其催化作用的研究 总被引：2，自引：0，他引：2

陈晓彬《邵阳学院学报(社会科学版)》2003,2(2):95-98

利用虾蟹壳为原料制备壳聚糖硫酸盐，探索以其为摧化剂的多相酯化反应催化体系的影响因素．通过研究，探索出催化剂的催化效能与壳聚糖的分子量和脱乙酰度的关系，并得出以壳聚糖硫酸盐为催化剂的酯化反应多相催化体系的应用范围和主要影响因素．相似文献

5.

酸介质对从废弃对虾壳中提取甲壳素工艺的影响