排序方式： 共有16条查询结果，搜索用时 1 毫秒
1.
A newly isolated strain EL31410, producing elastase (E. C3. 4. 4. 7) with high elastolytic activity was identified asBacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen
source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production.
A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite
experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran
and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but
soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4% (w/v) wheat bran and 9.4%
(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase
production in the optimized medium composition was also described.
Project (No. 300024) supported by the Zhejiang Provincial Natural Science Foundation of China 相似文献
2.
INTRODUCTION Elastase is an enzyme that attacks and solubi-lizes elastin. As elastase can degrade elastin (Mori-hara, 1967) that other proteases cannot; it has broad applications in medical therapy, food processing and daily use chemicals industry. Considerable eff- orts were made to screen the elastase-producing strains, to study its pathogen effect and its charac-terizations (Tsuzuki and Oka, 1965; Tsai et al., 1988; Sharon et al., 1997; Ozaki and Shiio, 1975). Shiio, 1975). Reporte… 相似文献
3.
The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bio-reactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ during earlier stage of cultivation. The maximum value [5.5 U/(h-g DCW)] of elastase formation rate occurred at 24 h at 30 ℃ compared to 4.6 U/(h-g DCW) at 30 h at 37 ℃. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and os 相似文献
4.
Sequential methodology based on the application of three types of experimental designs was used to optimize the fermentation conditions for elastase production from mutant strain ZJUEL31410 of Bacillus licheniformis in shaking flask cul- tures. The optimal cultivation conditions stimulating the maximal elastase production consist of 220 r/min shaking speed, 25 h fermentation time, 5% (v/v) inoculums volume, 25 ml medium volume in 250 ml Erlenmeyer flask and 18 h seed age. Under the optimized conditions, the predicted maximal elastase activity was 495 U/ml. The application of response surface methodology resulted in a significant enhancement in elastase production. The effects of other factors such as elastin and the growth factor (corn steep flour) on elastase production and cell growth were also investigated in the current study. The elastin had no significant effect on enzyme-improved production. It is still not clear whether the elastin plays a role as a nitrogen source or not. Corn steep flour was verified to be the best and required factor for elastase production and cell growth by Bacillus licheniformis ZJUEL31410. 相似文献
5.
This work applied the ultrasonic bonding to package flip chip GaN-based light emitting diodes (flip chip LEDs) on Si substrates.
The effects of ultrasonic bonding parameters on the reliability of flip chip GaN-based LED were investigated. In the sequent
aging tests, samples were driven with a constant current of 80 mA for hundreds hours at the room temperature. It was found
that the electroluminescence (EL) intensity variation had a large correlation to the ultrasonic power, and then to the bonding
temperature and force. A high bonding temperature and ultrasonic power and a proper bonding force improved the EL intensity
significantly. It was contributed to a strong atom inter-diffusion forming a stable joint at the bonding interface. The temperature
fluctuation in the aging test was the main factor to generate a high inner stress forming delamination at the interface between
the chip and Au bump. As a result, delamination had retarded the photons to emit out of the LED packaging and decay its EL
intensity. 相似文献
6.
目的:在大肠杆菌(BL21)中构建可溶性表达的金黄色葡萄球菌B型肠毒素(SEB)受体拮抗剂。方法:首先确定SEB受体桔抗剂的基因序列,然后用含有SEB受体桔抗剂的基因序列重组质粒表达载体PGEX-4T-1转化大肠杆菌BL21(DE3),利用IPTG诱导表达获得蛋白,产物经GST柱纯化后,利用ELISA检测其与SEB的结合能力并进行其体内外药效学实验。结果:该质粒成功转化为可溶性表达,ELISA结果显示表达产物可与SEB特异性结合。结论:本研究成功对SEB受体拮抗剂GST可溶性表达并对其活性进行初步分析。 相似文献
7.
An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K2HPO4, MgSO4@7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM,showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K2HPO4 0.206 g/100 ml and MgSO4@7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth. 相似文献
8.
An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies
by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize
the medium constituents. A central composite design was used to explain the combined effect of three medium constituents,
viz, glucose, K2HPO4, MgSO4·7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium.
The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour
0.616 g/100 ml, K2HPO4 0.206 g/100 ml and MgSO4·7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found
that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase
production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which
can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase
production and cell growth.
Project (No. 300024) supported by the Natural Science Foundation of Zhejiang Province, China 相似文献
9.
针对双PWM电机驱动系统中,因电机运行状态的频繁改变而引起．的直流母线电压的波动问题．通过分析直流电压波动的原因,提出了一种基于EL模型和负载功率前馈的双PWM控制策略．通过对整流器进行非线性建模、加入直流稳态电压补偿和采用负载功率预测方式,从而可以用较小的直流电容获得稳定的直流电压和网侧单位功率因数,实现能量的双向流动,仿真分析验证了该方法的正确性． 相似文献
10.
晶体硅光伏组件在户外长期运行时,各种材料会出现性能衰退。通过探究一批使用约30年的多晶硅组件,发现其在户外使用28年后衰减率仅为5.37%。此外,通过长期监测组件发电性能研究其在广州地区的发电和衰减情况,并采用EL测试研究材料衰变对组件影响,评估光伏组件的可靠性。 相似文献