排序方式: 共有118条查询结果,搜索用时 15 毫秒
1.
利用溶剂提取与DEAE—Sephadex A—25柱层析进行分离,根据高碘酸氧化、Smith降解,部分酸水解、甲基化及其产物的G.C.和G.C.—M.S.联机分析鉴定其结构.得出BJⅡa是多分枝结构的中性杂多糖,其主链是由1→2连接的葡萄糖和岩藻糖构成。 相似文献
2.
嗜水气单胞菌外分泌物的致病性 总被引:3,自引:0,他引:3
该菌分泌物有溶血性、细胞毒性和肠毒素毒性,毒性实验证实嗜水气单胞菌外分泌物对小白鼠和中华鳖有较高的致死毒性.层析纯化得到的1种蛋白质有上述3种毒性和致死性. 相似文献
3.
以胞内多糖产量为指标,对白灵菇胞内多糖液体发酵培养条件进行优化。在单因素和正交实验的基础上,确定发酵培养条件为:葡萄糖3.0%,蛋白胨0.2%,麸皮4.0%,KH2PO40.1%,MgSO40.1%,转速160r/min,初始pH6.5,接种量10%,装液量80mL/250mL,25℃培养时间6d,胞内多糖的产量达95.56mg/100mL。 相似文献
4.
目的:提取并纯化木防己多糖(AFMP),确定其组成,研究其清除活性氧自由基的作用.方法:利用水提醇沉得到木防己粗多糖,再经DEAE纤维素和聚酰胺纯制.利用Fenton反应研究AFMP清除·OH的作用;利用邻苯三酚自氧化法研究AFMP清除·O2-的作用.结果:纸色谱显示AFMP由葡萄糖和半乳糖组成.AFMP清除·OH的能力较弱;清除·O2-时的效果稍强,IC50为1407.4μg/ml.结论:木防己多糖为首次提取制得,有一定的清除羟自由基和超氧阴离子自由基的作用. 相似文献
5.
目前,国内外对甘薯活性物质的研究大都是以甘薯块根和甘薯茎蔓为对象,而对于甘薯生产的另外一批资源——甘薯根茎的研究却极为少见.其实,这是一部分生物量较大而又极具潜在价值的生物资源.用水作溶剂,采用破壁效果好的超声波技术提取甘薯根茎中的多糖,具有省时、环保、经济且不破坏功能成分结构等诸多优点,有着良好的发展前景. 相似文献
6.
7.
详细探讨了在碱催化下,采用环氧丙烷对罗望子多糖的羟丙基化改性工艺条件,并利用核磁共振氢谱和红外光谱对结构进行确认,结果表明:改性后的罗望子多糖胶粘度大幅下降,且具有良好的室温水溶解性。 相似文献
8.
采用热水浸提法提取显齿蛇葡萄多糖,用蒽酮-硫酸比色法测定糖含量,通过单因素试验研究提取温度、次数、时间及液料比对糖含量的影响,并采用L9(3^4)正交试验对提取工艺进行优化.以最佳工艺提取不同时期显齿蛇葡萄茎、叶的多糖并测定其含量.结果表明,对多糖含量的影响因素由大到小依次为提取温度、次数、时间和液料比.显齿蛇葡萄多糖... 相似文献
9.
巴戟天多糖对体外培养兔软骨细胞增殖的影响 总被引:4,自引:0,他引:4
如何能够促进软骨细胞的增殖是研究防治骨性关节炎(Osteoarthritis,OA)的重大课题.采用体外培养兔软骨细胞的方法,在培养过程中加入巴戟天多糖;实验结果证实巴戟天多糖能够促进软骨细胞增殖,以1.25 μg/mL的浓度为最优,巴戟天多糖促进软骨细胞的增殖作用可能与其使细胞增殖核抗原(PCNA)的表达增加有关. 相似文献
10.
Venkateshwari A Sri Manjari K Krishnaveni D Nallari P Vidyasagar A Jyothy A 《Indian journal of clinical biochemistry : IJCB》2011,26(2):136-139
Pancreatic fibrosis is a key pathological feature in the etiology of chronic pancreatitis that leads to obliteration of exocrine
and endocrine pancreatic tissues and its replacement by fibrous tissue resulting in clinical manifestations. Matrix metalloproteinase
9 is a member of the MMP family that is also known as gelatinase B, degrades type IV collagen of extracellular matrix and
basal membrane. The present study is aimed at evaluating the clinical significance of plasma concentration of MMP-9 in chronic
pancreatitis. The samples were obtained from 112 chronic pancreatitis patients and an equal number of age and sex matched
healthy controls. MMP-9 levels were quantitatively measured by ELISA assay. Statistical analysis was applied to test the significance
of results. The present study revealed a significant increase of plasma MMP 9 levels in chronic pancreatitis patients compared
to control subjects. Elevated levels were also observed in all the patient groups compared to control subjects with regard
to sex, age, addictions etc. MMP-9 degrades the type IV collagens in normal basement membrane, which in turn activates the
pancreatic stellate cells which promote the development of pancreatitic fibrosis. Thus, elevated plasma levels of MMP-9 may
act as a susceptibility factor for the development of chronic pancreatitis. 相似文献