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采用活体接种的方法,比较致病疫霉在辣椒和番茄上的致病性差异;结果表明致病疫霉在两种寄主上都能侵染,但是在番茄上的致病能力远远强与辣椒上的致病能力. 相似文献
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In October 2003, a new bacterial disease with symptoms similar to those caused by Xanthomonas axonopodis pv.poinsettiicola was observed on poinsettia leaves at a flower nursery in Zhejiang Province of China. Three Xanthomonas strains were isolated from infected plants and classified as X. axonopodis. They were differentiated from the pathotype strain LMG849 of X. axonopodis pv. poinsettiicola causing bacterial leaf spot of poinsettia by comparison of pathogenicity, substrate utilization and BOX-PCR genomic fingerprints. 相似文献
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Tong-bao Liu Guo-qing Chen Hang Min Fu-cheng Lin 《Journal of Zhejiang University. Science. B》2009,10(6):434-444
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight
of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino
acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses,
respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular
localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium
turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity
in M. oryzae.
Project supported by the National Natural Science Foundation of China (No. 30870101) and the Public Welfare Profession (Agriculture)
Research Project (No. 200803008), China 相似文献
4.
Po Po THAN Haryudian PRIHASTUTI Sitthisack PHOULIVONG Paul W.J. TAYLOR Kevin D. HYDE 《Journal of Zhejiang University. Science. B》2008,9(10)
Anthracnose disease is one of the major economic constraints to chilli production worldwide, especially in tropical and subtropical regions. Accurate taxonomic information is necessary for effective disease control management. In the Colletotrichum patho-system, different Colletotrichum species can be associated with anthracnose of the same host. Little information is known concerning the interactions of the species associated with the chilli anthracnose although several Colletotrichum species have been reported as causal agents of chilli anthracnose disease worldwide. The ambiguous taxonomic status of Colletotrichum species has resulted in inaccurate identification which may cause practical problems in plant breeding and disease management. Although the management and control of anthracnose disease are still being extensively researched, commercial eultivars of Capsicum annuum that are resistant to the pathogens that cause chilli anthracnose have not yet been developed. This paper reviews the causal agents of chilli anthracnose, the disease cycle, conventional methods in identification of the pathogen and molecular approaches that have been used for the identification of Colletotrichum species. Pathogenetic variation and population structure of the causal agents of chilli anthracnose along with the current taxonomic status of Colletotrichum species are discussed. Future developments leading to the disease management strategies are suggested. 相似文献
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草莓红中柱根腐病原致病性及杀菌剂毒力测定 总被引：1，自引：0，他引：1
为探究草莓红中柱根腐病的病原菌类型及筛选防治草莓红中柱根腐病适用药物,对皖北草莓种植区红中柱根腐病进行了调查、取样,并在室内进行病原菌分离、鉴定及致病性测定;同时,采用杀菌剂平板生物量法测定5种杀菌剂对草莓根腐病原菌的抑制中浓度EC50。结果表明,皖北草莓红中柱根腐病优势病原菌为立枯丝核菌及尖孢镰刀菌两种类型,58％雷多米尔EC50值为464．05,抑菌率最高;其次为95％溴菌清、50％异菌脲和80％乙磷铝;72．2％霜霉威EC50值为5715．58,抑菌率最差,EC50值大小为72．2％霜霉威〉80％乙磷铝〉50％异菌脲〉95％溴菌清〉58％雷多米尔。 相似文献
6.
Tong-bao LIU Guo-qing CHEN Hang MIN Fuc-heng LIN 《Journal of Zhejiang University. Science. B》2009,10(6)
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pl of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction ofconidiation, conidiai adhesion, appressorium turgot, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity in M. oryzae. 相似文献
7.
INTRODUCTION In recent years poinsettia cultivation has blos-somed from a small garden business into a multimil-lion-dollar industry in China. Some fungal diseases, such as Rhizoctonia stem and root rot, Pythium root rot, Fusarium wilt, and Botrytis blight have been reported to cause considerable damage during propagation (Sun et al., 2003). A new bacterial dis-ease characterized by leaf spot appearance was first observed on poinsettia in October 2003, in the Xiaoshan district of Zhej… 相似文献
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A rapidly growing number of successful genome sequencing projects in plant pathogenic fungi greatly increase the demands for tools and methodologies to study fungal pathogenicity at genomic scale. Magnaporthe oryzae is an economically important plant pathogenic fungus whose genome is fully sequenced. Recently we have reported the development and application of functional genomics platform technologies in M. oryzae. This model approach would have many practical ramifications in design and implementation of upcoming functional genomics studies of filamentous fungi aimed at understanding fungal pathogenicity. 相似文献
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Than PP Prihastuti H Phoulivong S Taylor PW Hyde KD 《Journal of Zhejiang University. Science. B》2008,9(10):764-778
Anthracnose disease is one of the major economic constraints to chilli production worldwide, especially in tropical and subtropical
regions. Accurate taxonomic information is necessary for effective disease control management. In the Colletotrichum patho-system, different Colletotrichum species can be associated with anthracnose of the same host. Little information is known concerning the interactions of the
species associated with the chilli anthracnose although several Colletotrichum species have been reported as causal agents of chilli anthracnose disease worldwide. The ambiguous taxonomic status of Colletotrichum species has resulted in inaccurate identification which may cause practical problems in plant breeding and disease management.
Although the management and control of anthracnose disease are still being extensively researched, commercial cultivars of
Capsicum annuum that are resistant to the pathogens that cause chilli anthracnose have not yet been developed. This paper reviews the causal
agents of chilli anthracnose, the disease cycle, conventional methods in identification of the pathogen and molecular approaches
that have been used for the identification of Colletotrichum species. Pathogenetic variation and population structure of the causal agents of chilli anthracnose along with the current
taxonomic status of Colletotrichum species are discussed. Future developments leading to the disease management strategies are suggested.
相似文献
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