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INTRODUCTION Ribulose-1,5-bisphosphate carboxylase/oxy-genase (Rubisco) is a key enzyme that initiates bothphotosynthetic and photorespiratory carbon me-tabolism. However, Rubisco has to be activated andcarbamylated to become catalytically competent.Rubisco activase (RCA) can alter the activity ofRubisco by facilitating the dissociation of tightlybound sugar phosphates from Rubisco in a processthat requires ATP hydrolysis. RCA, a chloroplastprotein encoded by a nuclea…  相似文献   
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本研究应用14C放射技术对1,5一二磷酸核酮糖羧化酶/加氧酶(Rubisco)活性进行了测定,并通过 SDS-PAGE不连续电泳凝胶技术对其蛋白亚基进行分离研究.结果发现:Rubisco的大亚基(LS)和小亚基(SS)在水稻叶片失绿过程中相对稳定,而其活性却有明显不同.说明 Rubisco在光合作用过程中的活性,除与自身的分子结构稳定性有关外,还与叶绿体中其他调节蛋白的存在密切相关.  相似文献   
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Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction inRubsico activase and/or the reduction in carbamylation.  相似文献   
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