排序方式： 共有33条查询结果，搜索用时 31 毫秒
1.
人体谷胱苷肽S-转移酶在肺和心血管系统免疫反应方面扮演一定角色,不少研究已经显示谷胱苷肽S-转移酶P1(glutathione S-transferaseP1,GSTP1)基因Ile105Val多态性可能与肺疾病的发生发展相关联,而该多态是否影响正常人体心肺功能少见报道。选取198名健康汉族成年男性,首次对GSTPl基... 相似文献
2.
Imbalanced free radicals and antioxidant defense systems in schizophrenia： A comparative study 总被引：2，自引：0，他引：2
Objective： To examine changes of blood oxidative-antiovidative level in schizophrenic patients and its relationship with clinical symptoms. Methods： Forty-six Chinese patients met DSM-IV （Diagnostic and Statistical Manual of Mental Disorders-iV） criteria for schizophrenia and fifty age- and sex-matched healthy controls were enrolled in the present study. Baseline psychiatric symptom severity was assessed with brief psychiatric rating scale, positive and negative syndrome scale on the blood draw day. Fresh blood samples were collected to measure levels of nitric oxide and lipid peroxide in plasma as well as activities of superoxide dismutase, catalase and glutathione peroxidase in red blood cells by spectrophotometric assays simultaneously. Results Comparison of the biochemical parameters indicated that the level of nitric oxide and lipid peroxide increased in patient group, which represented a positive correlation with positive scale scores; while the activities of three critical enzymes decreased and showed a negative linear correlation. Conclusion： This study showed that there are dysregulation of free radical metabolism and poor activities of the antioxidant defense systems in schizophrenic patients. Excess free radicals formation may play a critical role in the etiology of schizophrenia. Using antioxidants might be an effective therapeutic approach to partially alleviate or prevent the symptoms of schizophrenia. 相似文献
3.
Gora Dadheech Sandhya Mishra Shiv Gautam Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2006,21(2):34-38
A disturbance in the antioxidant defense system including α-tocopherol, ascorbic acid and reduced glutahtione metabolism due
to free radical induced oxidative injury has been implicated in various neuro-psychiatric disorders. The roles of these antioxidants,
changes in their blood levels and correlation with oxidative stress were studied in a common psychiatric illness Schizophrenia.
Fifty-eight subjects of either sex ranging in age from 18–60 years divided into two age groups (≤40 and >40 years) diagnosed
for schizophrenia, and forty age and sex-matched normal subjects as controls were included in the study. Blood samples were
analyzed for malondialdehyde (MDA), α-tocopherol, total ascorbic acid (TAA), dehydro ascorbic acid (DHAA), reduced ascorbic
acid (RAA), leucocyte ascorbic acid (LAA) and reduced glutathione (GSH). A decrease in the levels of α-tocopherol, total ascorbic
acid and reduced glutathione was found in schizophrenics compared to normal controls. Further a significant rise in oxidative
stress and decreased antioxidant status was observed in the chronic stage of schizophrenia as compared to those in acute condition.
A significant rise in dehydroascorbic acid with concomitant fall in reduced ascorbic acid suggests scavenging action of ascorbic
acid and its utilization with increased oxidative stress as indicated by high blood malondialdehyde levels. Leucocyte ascorbic
acid, a better index of ascorbic acid status was also found to be reduced in schizophrenics, suggesting depletion of body
stores of ascorbic acid and the condition worsened with advancing age. 相似文献
4.
K. S. Motghare Anil Bhutey B. B. Murrhar Madhur Gupta A. W. Meshram Y. Balsubramanium 《Indian journal of clinical biochemistry : IJCB》2001,16(2):213-215
With the growing interest in the concept of free radicals in the pathogenesis of myocardial ischemia, it was thought worthwhile
to study the changes in lipid peroxides and antioxidant enzyme glutathione peroxidase (GPx) in ischemic heart disease (IHD).
The study was carried out on 76 patients of IHD-38 of acute myocardial infarction (AMI) and 38 of stable ischemic heart disease
(SIDH). They were age and sex matched with 38 normal healthy controls. A significant increase (p<0.001) in lipid peroxides
as malondialdehyde (MDA) (5.9±0.7 mmol/L) and a decrease in GPx (24.6±2.2 U/gmHb) was found in patients of AMI when compared
with controls. There was no significant difference in these values in SIHD. Thus this study confirms the earlier findings
that MDA and GPx are useful parameters in IHD and their magnitude is dependent on severity and/or duration of ischemia. We
suggest that these tests would be of use in smaller institutions with limited facilities. 相似文献
5.
Thyagaraju K. Hemavathi B. Vasundhara K. Rao A. D. Devi K. N. 《Journal of Zhejiang University. Science. B》2005,6(8):759-769
A comparative study was made on the tissue specific expression of glutathione transferases (GST) in brain and testis after
exposure of rat to phenobarbitol (PB) and β-methylcholanthrene (MC). Glutathione transferases, a family of multifunctional
proteins are involved in intracellular transport processes and in detoxication of electrophilic xenobiotics by catalyzing
reactions such as conjugation, isomerization, reduction and thiolysis. On purification, the yield of GST proteins by affinity
chromatography was 39% in testis and 32% in brain. The affinity purified testis GSTs were resolved by chromatofocusing into
six anionic and four cationic isozymes, and in brain glutathione transferases were resolved into four anionic and three cationic
isozymes, suggesting the presence of multiple isozymes with Yc, Yb, Yβ and Yδ in both of them. In testis and brain, these
isozymes at identical pI values showed variable functions with a battery of substrates and the cationic isozymes of brain
and testis showed identical properties in CHP (cumene hydroperoxide) at pH values of above 7.0. Substrate specificity studies
and immunoblot analysis of testis and brain proteins revealed that they play a predominant role in the detoxication of phenobarbitol
or β-methylcholanthrene. Expression of the isozymes in testis and brain on exposure to PB and MC indicated elevated subunit
variation. In both testis and brain, Yδ of π class was expressed on PB treatment and Yc of α class and Yβ of μ class was expressed
in MC treated testis and only Yc was predominantly expressed in MC treated brain. Thus these subunits expression is considered
as markers for carcinogenesis and specific to chemical toxicity under phenobarbitol and β-methylcholanthrene stress.
Project supported by Department of Science and Technology and Council for Scientific and Industrial Research, New Delhi 相似文献
6.
Y. P. Singh O. P. Sachdeva S. K. Aggarwal K. Chugh Harbans Lal 《Indian journal of clinical biochemistry : IJCB》2008,23(3):290-292
Blood glutathione was estimated in fifty patients of head and neck cancer in the age group of 18–76 years and the results
were compared with a group of normal healthy controls. Mean blood glutathione level was found to be significantly lowered
in patients than the controls. Irrespective of the site, TNM classification, histopathology, and character of lesion, fall
in blood glutathione was nearly same in all the patients. The mean level was significantly increased after radiotherapy when
compared with the levels before radiotherapy. The decreased levels of GSH in-patients with head and neck cancer, observed
in the present study, may be due to its increased utilization by the cells. The results suggest that patients with head and
neck cancer have increased oxidative stress. 相似文献
7.
T. K. Sankaranarayanan C. Subramanyam 《Indian journal of clinical biochemistry : IJCB》2003,18(1):1-4
A comparative study was conducted to evaluate calcineurin activity in normal pancreatic beta cells and insulinoma cells in
relation to their oxidative state. In comparison to normal islets, insulinoma cells had enhanced oxidative stress as evidenced
by increased content of thiobarbituric acid reactive substances. In addition, diminished activity of calcineurin in insulinoma
cells was concomitant with decreased content of reduced glutathione and glutathione peroxidase activity signifying diminished
antioxidant status in these cells. Culturing insulinoma cells in presence of the calcineurin inhibitor cyclosporin A resulted
in further decrease of calcineurin activity with restoration of glutathione peroxidase but without restoration of reduced
glutathione levels. These results indicate that an estimate of oxidative stress in pancreatic islets and insulinoma cells
can be obtained by assaying calcineurin activity. 相似文献
8.
Subir Kumar Das Hiran K. R. Sukhes Mukherjee D. M. Vasudevan 《Indian journal of clinical biochemistry : IJCB》2007,22(1):99-104
Damaging effects of reactive oxygen species on living systems are well documented. They include oxidative attack on vital
cell constituents. Chronic ethanol administration is able to induce an oxidative stress in the central nervous system. In
the present study, 16–18 week-old male albino rats of Wistar strain were exposed to different concentration of ethanol for
4 weeks. This exposure showed profound effect on body weight. Ascorbic acid level; and activities of alkaline phosphatase
and aspartate transaminase in the brain are dependent on the concentration of ethanol exposure. Chronic ethanol ingestion
elicits statistically significant increase in thiobarbituric acid reactive substances level and decrease in gluatathione level
in the brain. It reduces superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activities in a
dose dependent manner. However, histological examination could not reveal any pathophysiological changes. Therefore, we conclude
that biochemical alterations and oxidative stress related parameters respond early in alcoholism than the histopathological
changes in brain. 相似文献
9.
Lipid peroxidation by measurement of Thiobarbituric Acid Reactive Substances (TBARS) and antioxidant status by determining
the activities of the enzymes, Catalase (Cat), Glutathione Peroxidase (GPx) and Superoxide Dismutase (SOD) and the level of
Reduced Glutathione (GSH) in the erythrocytes of patients with type 2 Diabetes Mellitus were investigated. It was observed
that the level of TBARS in the erythrocytes was increased by 50% showing a significant generation of free radicals in the
erythrocytes of these patients. The activities of both Cat and SOD were enhanced while that of GPx was not altered. The level
of GSH was also not changed. 相似文献
10.
Ya-wei Li Ze-hua Gong Yao Mu Yi-xian Zhang Zeng-jie Qiao Li-ping Zhang Zhu-ping Jin Hua Li Yan-xi Pei 《Journal of Zhejiang University. Science. B》2012,13(12):1006-1014
The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production.In our studies,we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis.Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis.The expression of some genes responsible for Cd2+ transportation into vacuoles was induced,and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment.The expression of Cd-transportation related genes was not in response to Cd-stress,whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment.The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment.Additionally,malondialdehyde (MDA),hydrogen peroxide,and Cd2+ contents,and activities of some antioxidative enzymes,differed between the wild type and atcsr-2.Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years.The findings revealed that the expression pattern of H2 S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2,and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity.Finally,one possible suggestion was given:down-regulation of atcsr-2,depending on H2S gas-transmitter not only weakened Cd2+ chelation,but also reduced Cd2+ transportation into vacuoles,as well as enhancing the Cd2+ assimilation,thus rendering atcsr-2 mutant sensitive to Cd-stress. 相似文献