针刺和静力牵张对大负荷运动后骨骼肌M线变化影响的免疫电镜研究   总被引：4，自引：1，他引：3  

屈竹青  樊景禹  卢鼎厚 《体育科学》1992,(6)

用胶体金标记技术定位运动后及运动后经针刺或静力牵张处理的人骨骼肌M蛋白,以探讨超过习惯负荷后骨骼肌收缩结构变化以及针刺和静力牵张对促进其恢复的机制。我们发现:大负荷斜蹲后未经针刺的对照腿股外肌M线的M蛋白标记密度低于针刺腿(P<0.05),而对照腿M线两侧及M线两侧以外的M蛋白标记密度高于针刺腿(P<0.05)。对照实验证明运动腿M线以外增加的标记物为物异性标记。静力牵张组在M线及M线两侧的标记结果与针刺组一致。仅在M线两侧以外区域牵张腿与运动对照腿之间标记密度的差异没有统计学意义。我们还观察到运动后对照腿变化M线体密度达42—48％,而针刺或牵张腿为6—9％。上述结果表明大负荷运动加强了负荷后骨骼肌M蛋白的解聚或降解,从而导致M线结构改变;针刺和静力牵张可显著促进M蛋白的合成或抑制其降解,而使M线结构恢复或稳定。本文还探讨了M线变化与肌粗丝结构变化的关系。  相似文献   

Seeking Nettle Textiles – Utilizing a Combination of Microscopic Methods for Fibre Identification     

Jenni A. Suomela  Krista Vajanto  Riikka Räisänen 《文物保护研究》2018,63(7):412-422

Bast fibres have been commonly used as a textile material in Northern Europe since Neolithic times. However, the process of identifying the different species has been problematic, and many important questions related to their cultural history are still unanswered. For example, a modified Herzog test and the presence of calcium oxalate crystals have both been used in identification. In order to generate more reliable results, further research and advancement in multi-methodological methods is required. This paper introduces a combination of methods which can be used to identify and distinguish flax (Linum usitatissimum), hemp (Cannabis sativa), and stinging nettle (Urtica dioica). The research material consisted of reference fibres and 25 fibre samples obtained from 12 textiles assumed to be made of nettle. The textiles were from the Finno-Ugric and Historical Collections of The National Museum of Finland. The fibre samples were studied by observing the surface characteristics and cross sections with transmitted light microscopy, and by using a modified Herzog test with polarized light, in order to identify the distinguishable features in their morphological structures. The study showed that five out of 25 samples were cotton, 16 nettle, one flax, and one hemp. Findings from two samples were inconsistent. The results show that it is possible to distinguish common north European bast fibres from each other by using a combination of microscopic methods. Furthermore, by utilizing these combined methods, new and more reliable information could be obtained from historical ethnographic textiles, which creates new vistas for the interpretation of their cultural history.  相似文献   

Virtual Microscopy as a Learning Tool in Brazilian Medical Education     

Fernanda Somera dos Santos  Mariana Kiomy Osako  Gleici da Silva Castro Perdoná  Márcia Gaião Alves  Katiuchia Uzzun Sales 《Anatomical sciences education》2021,14(4):408-416

Virtual microscopy (VM) is a widely used teaching method in Medical Education in many developed countries. In Brazil, however, this is not the case for most medical schools, considering Brazilian social inequality and uneven access to technology. Recently, the Covid-19 pandemic has also challenged Universities to seek and make a transition toward more effective methods of full-time online education. Thus, the main goal of this work was to verify student's perception and academic performance, assessed upon VM implementation in a Brazilian Medical School. Ribeirao Preto Medical School students answered a 26-question survey with regards to optical microscopy (OM) and VM. Academic performance was compared between participants that were (year of 2019) or were not (year of 2015) exposed to VM. Taken the results together, subjective impressions such as handling, suitability, learning effectiveness, and pleasure using the tools, have shown a higher score for virtual microscopy (median = 29), when compared to optical microscopy (median = 24) with a P-value < 0.001 by Wilcoxon rank test, upon measurement using an ordinal scale. Regarding academic performance, no statistically significant differences were found between groups (P-value = 0.38, Cohen's d = 0.19). Therefore, VM proved to be adequate to the Brazilian medical education in light of Brazilian social contexts and Covid-19 pandemic.  相似文献   

Evaluating a technology supported interactive response system during the laboratory section of a histology course          下载免费PDF全文

Vera D. Rinaldi  Nancy A. Lorr  Kimberly Williams 《Anatomical sciences education》2017,10(4):328-338

Monitoring of student learning through systematic formative assessment is important for adjusting pedagogical strategies. However, traditional formative assessments, such as quizzes and written assignments, may not be sufficiently timely for making adjustments to a learning process. Technology supported formative assessment tools assess student knowledge, allow for immediate feedback, facilitate classroom dialogues, and have the potential to modify student learning strategies. As an attempt to integrate technology supported formative assessment in the laboratory section of an upper‐level histology course, the interactive application Learning Catalytics^TM, a cloud‐based assessment system, was used. This study conducted during the 2015 Histology courses at Cornell University concluded that this application is helpful for identifying student misconceptions “on‐the‐go,” engaging otherwise marginalized students, and forming a new communication venue between students and instructors. There was no overall difference between grades from topics that used the application and grades from those that did not, and students reported that it only slightly helped improve their understanding of the topic (3.8 ± 0.99 on a five‐point Likert scale). However, they highly recommended using it (4.2 ± 0.71). The major limitation was regarding the image display and graphical resolution of this application. Even though students embrace the use of technology, 39% reported benefits of having the traditional light microscope available. This cohort of students led instructors to conclude that the newest tools are not always better, but rather can complement traditional instruction methods. Anat Sci Educ 10: 328–338. © 2016 American Association of Anatomists.  相似文献   

布儒斯特角显微镜和单分子膜表面形貌研究技术     

刘明贤  甘礼华  陈根  王小兰  陈龙武 《实验室研究与探索》2005,24(9):15-16

用布儒斯特角显微镜研究了花生酸分子成膜过程中单分子膜表面形貌的动态变化，发现在气／液界面上花生酸分子首先形成岛状分布，随着表面压的增加形成完整的单分子膜，而当表面压达到崩溃压后薄膜出现裂纹，分子膜重叠，形成多层膜。分析了花生酸单分子膜的形成机理。  相似文献   

Clinical Case-Based Image Portfolios in Medical Histopathology     

Thomas S. King  Ramaswamy Sharma  Jeff Jackson  Kristin R. Fiebelkorn 《Anatomical sciences education》2019,12(2):200-209

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Dynamics and manipulation of ferroelectric domain walls in bismuth ferrite thin films     

Shuyu Xiao  Yaming Jin  Xiaomei Lu  Sang-Wook Cheong  Jiangyu Li  Yang Li  Fengzhen Huang  Jinsong Zhu 《国家科学评论(英文版)》2020,7(2):278

Ferroelectric domain walls differ from domains not only in their crystalline and discrete symmetry, but also in their electronic, magnetic, and mechanical properties. Although domain walls provide a degree of freedom to regulate the physical properties at the nanoscale, the relatively lower controllability prevents their practical applications in nano-devices. In this work, with the advantages of 3D domain configuration detection based on piezoresponse force microscopy, we find that the mobility of three types of domain walls (tail-to-tail, head-to-tail, head-to-head) in (001) BiFeO₃ films varies with the applied electrical field. Under low voltages, head-to-tail domain walls are more mobile than other domain walls, while, under high voltages, tail-to-tail domain walls become rather active and possess relatively long average lengths. This is due to the high nucleation energy and relatively low growth energy for charged domain walls. Finally, we demonstrate the manipulation of domain walls through successive electric writings, resulting in well-aligned conduction paths as designed, paving the way for their application in advanced spintronic, memory and communication nano-devices.  相似文献   

Chinese archaeological artefacts: Microstructure and corrosion behaviour of high-leaded bronzes     

《Journal of Cultural Heritage》2014,15(3):283-291

Metallographic features of ancient bronze artefacts often hide peculiar micro-chemical processes and corrosion behaviours, which are worth to be studied as they can provide conservators and archaeologists with valuable tools and information. It is widely documented that Chinese bronzes were cast and the way to adjust their properties was to change the alloy composition. In particular, addition of lead, which is insoluble in the bronze matrix, results in the formation of inclusions or globules, which undergo oxidation processes leading to their conversion into corrosion products. The mechanisms through which this occurs were still poorly investigated. The present work was conducted to further study the corrosion behaviour of high-leaded bronze, especially focusing on the behaviour of lead globules. To this aim, a collection of Chinese archaeological bronzes, showing intermediate steps of degradation, were selected and investigated. The use of combined microscopy-based, molecular and elemental, analytical techniques allowed the characterization as well as the precise location of corrosion products, thus enabling us to propose a degradation pathway basing on thermodynamic data provided by Pourbaix diagram. The achieved results will be useful for researchers involved in these kinds of studies to better interpret data obtained.  相似文献   

A prospective,randomized crossover study comparing direct inspection by light microscopy versus projected images for teaching of hematopathology to medical students          下载免费PDF全文

Aaron M. Carlson  Ellen D. McPhail  Vilmarie Rodriguez  Georgene Schroeder  Alexandra P. Wolanskyj 《Anatomical sciences education》2014,7(2):130-134

Instruction in hematopathology at Mayo Medical School has evolved from instructor‐guided direct inspection under the light microscope (laboratory method), to photomicrographs of glass slides with classroom projection (projection method). These methods have not been compared directly to date. Forty‐one second‐year medical students participated in this pilot study, a prospective, randomized, crossover study measuring educational performance during a hematology pathophysiology course. The students were randomized to one of two groups. All students received the same didactic lectures in the classroom and subsequent case‐based review of peripheral blood smears using either laboratory or projection methods, on day one with a crossover to the other method on day two. Pre‐ and post‐test examinations centered on morphology recognition measured educational performance on each day, followed by a questionnaire identifying the student's favored method. There was no significant difference in the pre‐test and post‐test scores between the two teaching methods (rank‐sum P = 0.43). Students overwhelmingly preferred the projection method and perceived it as superior (76%), although post‐test scores were not significantly different. Student's recommended method was split with 50% favoring the projection method, 43% favoring a combined approach, and 23% noting logistical challenges to the laboratory. In this study, the laboratory and projection method were equivalent in terms of educational performance for hematopathology among medicals students. A classroom‐based approach such as the projection method is favored, given the large class sizes in undergraduate medical education, as well as the ergonomic challenges and additional resources required for large group instruction in a laboratory setting. Anat Sci Educ 7: 130–134. © 2013 American Association of Anatomists.  相似文献   

Evaluation of usage of virtual microscopy for the study of histology in the medical,dental, and veterinary undergraduate programs of a UK University          下载免费PDF全文

Margaret K. Gatumu  Frances M. MacMillan  Philip D. Langton  P. Max Headley  Judy R. Harris 《Anatomical sciences education》2014,7(5):389-398

This article describes the introduction of a virtual microscope (VM) that has allowed preclinical histology teaching to be fashioned to better suit the needs of approximately 900 undergraduate students per year studying medicine, dentistry, or veterinary science at the University of Bristol, United Kingdom. Features of the VM implementation include: (1) the facility for students and teachers to make annotations on the digital slides; (2) in‐house development of VM‐based quizzes that are used for both formative and summative assessments; (3) archiving of teaching materials generated each year, enabling students to access their personalized learning resources throughout their programs; and (4) retention of light microscopy capability alongside the VM. Student feedback on the VM is particularly positive about its ease of use, the value of the annotation tool, the quizzes, and the accessibility of all components off‐campus. Analysis of login data indicates considerable, although variable, use of the VM by students outside timetabled teaching. The median number of annual logins per student account for every course exceeded the number of timetabled histology classes for that course (1.6–3.5 times). The total number of annual student logins across all cohorts increased from approximately 9,000 in the year 2007–2008 to 22,000 in the year 2010–2011. The implementation of the VM has improved teaching and learning in practical classes within the histology laboratory and facilitated consolidation and revision of material outside the laboratory. Discussion is provided of some novel strategies that capitalize on the benefits of introducing a VM, as well as strategies adopted to overcome some potential challenges. Anat Sci Educ 7: 389–398. © 2013 American Association of Anatomists.  相似文献