| Meso-四-(3-5-二溴4-羟基苯)卟啉分光光度法测食品中铅含量 |
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| 引用本文： | 陈莉,彭惠琦.Meso-四-(3-5-二溴4-羟基苯)卟啉分光光度法测食品中铅含量[J].实验室研究与探索,2011,30(11):44-47,60. |
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| 作者姓名： | 陈莉 彭惠琦 |
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| 作者单位： | 华东师范大学化学系,上海,200241 |
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| 摘 要： | 以食品为对象,研究了铅与Meso-四-(3-5-二溴-4-羟基苯)卟啉T(DBHP)P]的配合反应,并对于溶液pH值、加热时间、表面活性剂用量、显色剂用量等对配合物的生成及稳定性的影响进行了探究,确定反应的最佳条件.经试验,当8-羟基喹啉存在时,铅在碱性条件下可与T(DBHP)P反应生成1:2的橙黄色化合物.该化合物在479 nm处有较强的吸收,当铅含量在0～12 μg/mL时,测得的曲线呈线性关系,服从比尔定律,重复性较好.经分光光度计测出其吸光度,绘制标准工作曲线,计算出铅的含量.结果表明:本实验所采用方法简便快速、安全,对环境污染小,且灵敏度较高,选择性较好,样品不需要分离出干扰离子,只需加入掩蔽剂即可消除钙离子和铁离子的影响.将该方法用于测定食品中铅的含量,取得了较满意的效果.
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| 关 键 词： | Meso-四-(3-5-二溴-4-羟基苯)卟啉 分光光度法 铅 |
Spectrophotometric Determination of Lead in Food with Meso-4-(3-5-Dibromo-4-hydroxyphenyl) Porphyrin [T(DBHP)P] |
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| CHEN Li
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PENG Hui-qi.Spectrophotometric Determination of Lead in Food with Meso-4-(3-5-Dibromo-4-hydroxyphenyl) Porphyrin [T(DBHP)P][J].Laboratory Research and Exploration,2011,30(11):44-47,60. |
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| Authors: | CHEN Li PENG Hui-qi |
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| Institution: | CHEN Li,PENG Hui-qi(College of Chemistry,East China Normal University,Shanghai 200241,China) |
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| Abstract: | Reaction of Meso-4-(3-5-Dibromo-4-hydroxyphenyl) porphyrin T(DBHP)P] and the lead in the foods,and the impact of heating time,pH,dosage of the surfactants and reagent on the formation and stability of the complex are studied,and the best reaction conditions are determined.With the presence of 8-hydroxyquinoline,lead can create 1∶ 2 orange compounds with T(DBHP)P.Which has strong absorption of 479 nm.When the lead content is in the range of 0~12 μg/mL,the measured curve is linear.The results showed that the method used is simple,safe,more environmental friendly with higher sensitivity and better selectivity.There is no need to separate the interfering ions in the sample.Simply by adding a masking agent,the calcium and iron effects can be eliminated.The spectrophotometer measures absorbance and the standard curve to calculate the lead content.This method can be used to measure the lead in foods with ease. |
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| Keywords: | Meso-4-(3-5-Dibromo-4-hydroxyphenyl) porphyrin [T(DBHP)P] spectrophotometry lead |
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