| 黄粉虫几丁质酶的纯化及性质 |
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| 引用本文： | 谢晓兰,曾炜,高平章,陈显柱,陈小莲.黄粉虫几丁质酶的纯化及性质[J].泉州师范学院学报,2012,30(4):56-61. |
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| 作者姓名： | 谢晓兰 曾炜 高平章 陈显柱 陈小莲 |
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| 作者单位： | 泉州师范学院化学与生命科学学院福建省分子生物与药物化学重点实验室,福建泉州,362000 |
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| 基金项目： | 泉州市科技计划项目(2011Z17);泉州师院大学生科研基金项目(2011DKJ08) |
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| 摘 要： | 以黄粉虫为材料,采用盐析、亲和柱层析、Sephadex G-100分子筛及DE-52离子交换柱层析等制得纯化倍数约33倍,回收率为38%,比活力为294.7U/mg的几丁质酶制剂.在此基础上,以胶体几丁质为底物,考察黄粉虫几丁质酶的性质.结果表明：该酶催化水解几丁质的Km值为71.4mg/L;酶的最适pH值是6.0;最适温度为45℃左右;酶在pH 5.0～7.0,45℃以下酶活力稳定性较好.Na＋和K＋对酶活无影响;Ca2＋、Cu2＋对酶活起先扬后抑作用;Mn2＋、Mg2＋、Fe3＋、Al3＋对该酶活力均具有不同程度的抑制作用.
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| 关 键 词： | 黄粉虫 几丁质酶 纯化 酶学性质 |
Characterization and Purification of Chitinase from Terebrio molitor Linneeus |
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| XIE Xiao-lan,ZENG Wei,GAO Ping-zhang,CHEN Xian-zhu,CHEN Xiao-lian.Characterization and Purification of Chitinase from Terebrio molitor Linneeus[J].Journal of Quanzhou Normal College,2012,30(4):56-61. |
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| Authors: | XIE Xiao-lan ZENG Wei GAO Ping-zhang CHEN Xian-zhu CHEN Xiao-lian |
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| Institution: | (Key Lab of Fujian Province for Molecular Biology and Medicinal Chemistry, College of Chemistry and Life Sciences,Quanzhou Normal University,Fujian 362000,China) |
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| Abstract: | In this paper,Chitinase from Tenebrio molitor Linneeus was purified by extraction and ammonium sulfate fractionation,then chromatography on chitin Affinity column followed by Sephadex G-100 and DEAE-cellulose(DE-52) columns.The specific activity of the purified chitinase was 294.7 U/mg.the recovery rate of chitinase was 38%,and the multiple of purification was about 33 times.The characterization of chitinase from Terebrio molitor Linneeus was studied.The Michaelis constant Km value of chitinase was determinded to be 71.4 mg/L.The optimum pH and optimum temperature of chitinase for the hydrolysis of colloidal chitin(enzyme substrate) were determined to be pH 6.0 and 45 ℃,respectively.The stability of chitinase was investigated,and the results show that the enzyme is stable in a pH range from 5.0 to 7.0 and at temperatures under 45 ℃.The effects of metal ions on the enzyme were also studied.The results indicates that Na+ and K+ have no any effect on the enzyme activity.But Ca2+ and Cu2+ activates the enzyme activity at low concentration,and they inactivate it at high concentrations.Mn2+,Mg2+,Fe3+,Al3+ inhibite the enzyme. |
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| Keywords: | tenebrio molitor linneeus chitinase purification characterization |
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