| 光照及黑暗条件下不同浓度2，4-D对诱导苦马豆愈伤组织的影响 |
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| 引用本文： | 周吉林,伍春莲,陈卫英.光照及黑暗条件下不同浓度2，4-D对诱导苦马豆愈伤组织的影响[J].内江师范学院学报,2009,24(12):41-44. |
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| 作者姓名： | 周吉林 伍春莲 陈卫英 |
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| 作者单位： | 1. 广安职业技术学院生物系,四川,广安,638000
2. 西华师范大学生命科学学院,四川,南充,637002 |
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| 基金项目： | 四川省教育厅青年基金项目 |
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| 摘 要： | 以苦马豆幼苗的子叶及下胚轴为外植体，研究了光照及黑暗条件下不同浓度2，4-D对苦马豆愈伤组织生长量的影响．结果表明：予叶愈伤组织的生长均优于下胚轴愈伤组织的生长，2，4-D浓度为0．3，0．6mg／L时，子叶、下胚轴愈伤组织的生长光培养优于暗培养；在2，4-D浓度为1．2mg／L时，子叶、下胚轴愈伤组织的生长暗培养优于光培养．因此，以2，4-D单激素诱导苦马豆愈伤组织，子叶培养在暗处是最好的选择．
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| 关 键 词： | 2，4-D 苦马豆 愈伤组织 鲜重 生物量 |
Effects of Different C0ncentration Of 2,4-D on Callus Induction of Swainsona Salsula under Bright or Dark Conditions |
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| ZHOU Ji-ling,WU Chun-lian,CHEN Wei-ying.Effects of Different C0ncentration Of 2,4-D on Callus Induction of Swainsona Salsula under Bright or Dark Conditions[J].Journal of Neijiang Teachers College,2009,24(12):41-44. |
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| Authors: | ZHOU Ji-ling WU Chun-lian CHEN Wei-ying |
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| Institution: | ZHOU Ji-ling , WU Chun-lian , OHEN Wei-ying ( 1. Deparment of Biology, Guangan Vocational-technical College, Guang＇ an, Siehuan 638000, China; 2. School of Life Sciences, China West Normal University, Nanehong, Siehuan 637002, China) |
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| Abstract: | Swainsona salsula is an important medicinal plant. Using the cotyledons and hypoeotyls of Swainsonia salsula as explants, the effect of different concentration of 2,4-D on callus induction of Swainsona salsula＇s growth under bright or dark conditions was researched,. The results showed that the callus＇s growth of the cotyledons was greater than the hypoeotyls＇s. In 0.3mg/L, 0.6mg/L 2, 4-D, bright conditions were found to have a better result than dark conditions ~ in 1.2mg/L 2, 4-D, better results were observed under dark conditions than under bright conditions. Therefore, if the callus of Swainsona salsula was induced by 2, 4-D, the growth of cotyledons was better under dark conditions. |
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| Keywords: | 2 4-D |
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