| 纤维素分解菌的分离筛选和产酶条件优化 |
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| 引用本文： | 周红霞,江海涛,张红琳,陈聪颖.纤维素分解菌的分离筛选和产酶条件优化[J].南京晓庄学院学报,2010,26(6):37-40. |
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| 作者姓名： | 周红霞 江海涛 张红琳 陈聪颖 |
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| 作者单位： | 南京晓庄学院生物化工和环境工程学院,江苏南京211171 |
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| 基金项目： | 江苏省农业三项工程项目 |
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| 摘 要： | 目的：研究纤维素分解菌的分离及产酶条件优化.方法：以含菌秸秆、腐木叶和玉米地土壤为原料,经富集、初筛培养后,根据水解圈直径/培养天数进行纤维素分解菌的复筛培养,测定CMC酶活和FPA滤纸酶活,并进行了产酶条件的单因素优化实验.结果：采集的样品中共分离到4个菌株,均为细菌,其中H-3的羧甲基纤维素（CMC）酶活和滤纸（FPA）酶活最高,分别是0.5401U.m l-1、0.2923U.m l-1.结论：H-3菌分解纤维素的能力最强,最佳产酶条件为温度30℃,pH4.5.不含尿素,葡萄糖和纤维素含量为0.4%和0.6%.
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| 关 键 词： | 纤维素分解菌 分离 筛选 CMC酶活 滤纸酶活 |
Screening on the Cellulose-decomposing Microorganisms and Optimization of Fermentation Conditions |
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| ZHOU Hong-xia,JIANG Hai-tao,ZHANG Hong-lin,CHEN Cong-ying.Screening on the Cellulose-decomposing Microorganisms and Optimization of Fermentation Conditions[J].Journal of Nanjing Xiaozhuang College,2010,26(6):37-40. |
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| Authors: | ZHOU Hong-xia JIANG Hai-tao ZHANG Hong-lin CHEN Cong-ying |
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| Institution: | (School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,Jiangsu) |
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| Abstract: | The study is aimed to screen cellulose decomposing microorganisms with good decomposition effect.With straw that contained microorganisms,rotten leaves and soil in maize field as materials,after the enrichment culture and primary screening,the microorganism was isolated according to the diameter of hydrolyzed circle/cultured days.The crude enzyme liquid was prepared for the determination of CMC Case activity and FPA.The optimized object was decided through changing conditions for the enzyme reaction.Then the single factor optimization was done.The results showed that 4 strains were isolated from the sample with one being better than others.The CMC Case activity and FPA of H-3 was 0.5401.It is concluded that strains H-3 has the strongest ability to decompose cellulose;the optimum conditions include ：the cultural temperature set at 30℃,pH at 4.5,absence of urea,the concentration of GLC and cellulose being 0.3% and 0.5% respectively. |
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| Keywords: | cellulose-decomposing microorganisms isolation screening CMC Case activity FPA |
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