| 葛渣异黄酮的提取及对细菌的抑制作用 |
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| 引用本文： | 徐毅君,黄彤.葛渣异黄酮的提取及对细菌的抑制作用[J].宜宾师范高等专科学校学报,2013(12):84-87. |
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| 作者姓名： | 徐毅君 黄彤 |
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| 作者单位： | 宜宾学院生命科学与食品工程学院,四川宜宾644007 |
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| 基金项目： | 四川省教育厅科研项目“葛粉生产废液及废渣的综合利用”（09ZA149） |
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| 摘 要： | 以葛根（radix puerariae）生产葛粉后的葛渣为材料,采用乙醇回流法通过正交实验对异黄酮提取条件进行优化,同时对葛根异黄酮对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑菌作用进行研究.结果表明：以乙醇作为提取溶剂,最佳提取条件为乙醇浓度95％,提取温度70℃,料液比1∶15,提取时间120 min,葛根异黄酮的得率为1.59％;葛根异黄酮对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的生长都具有抑制作用,最低抑菌浓度分别为125 μg/mL、62.5 μg/mL、250μg/mL,最低杀菌浓度分别为250 μg/mL、125 μg/mL、500 μg/mL.
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| 关 键 词： | 葛渣 异黄酮 提取 抑菌 |
The Extraction and Antibacterial Activity of Isoflavones from Root Dregs of Kudzu |
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| XU Yi-jun,HUANG Tong.The Extraction and Antibacterial Activity of Isoflavones from Root Dregs of Kudzu[J].Journal of Yibin Teachers College,2013(12):84-87. |
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| Authors: | XU Yi-jun HUANG Tong |
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| Institution: | (Institute of Life Science and Food Engineering, Yibin University, Yibin, Sichuan 644007, China) |
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| Abstract: | The ethanol refluxing method was used to extract isoflavones from the root dregs of kudzu （radix puerariae）. The ex- traction conditions were studied by orthogonal design experiments. The antibacterial activity of isoflavones to escherichia coli, staphylococcus aureus, and bacillus subtilis was also studied. The experimental results show that： using ethanol as extraction sol- vent, the optimum extraction conditions is ethanol concentration 95%, extraction temperature 70 ℃, material-to-liquid ratio 1 ： 15, extraction time 120 min. Under this condition, the yield of isoflavones extracting from root dregs of kudzu is 1.59%. Isofla- vones has inhibitory effect on the growth of escherichia coli, staphylococcus aureus and bacillus subtilis. The minimal inhibitory concentration （MIC） is 125 μg/mL, 62.5 μg/mL and 250 μg/mL separately; the minimum bactericidal concentration （MBC） is 250 μg/mL, 125 μg/mL and 500 Ixg/mL separately. |
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| Keywords: | root dregs of kudzu isoflavones extraction antibacterial activity |
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