| HPLC法测定骨愈灵片中人参皂苷Rg1和三七皂苷R1的含量 |
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| 引用本文： | 张冕.HPLC法测定骨愈灵片中人参皂苷Rg1和三七皂苷R1的含量[J].荆门职业技术学院学报,2009,24(2):43-46. |
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| 作者姓名： | 张冕 |
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| 作者单位： | 荆楚理工学院,化工与药学院,湖北,荆门,448000
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| 摘 要： | 目的：建立骨愈灵片中人参皂苷Rg1和三七皂苷R1的含量测定方法。方法：采用高效液相色谱法，乙腈-水（18：82）为流动相；流速1．0mL·min^-1；检测波长203131／1。结果：人参皂苷Rg1的回归方程为Y=2．829X＋4．167（r=0．9999，n=5），线性范围为3．0～22．5ug；三七皂苷R1的回归方程为Y=7．580X-3．971（r=0．9996，n=5），线性范围为1．0～7．5ug。平均回收率分别为98．95％、99．27％。结论：本方法准确、灵敏，可用于骨愈灵片的质量控制。
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| 关 键 词： | 骨愈灵片 人参皂苷Rg1 三七皂苷R1 HPLC |
Determination of Ginsenoside Rg_1 and Notoginsecoside R_1 in Guyuling Tablets by HPLC |
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| ZHANG Mian.Determination of Ginsenoside Rg_1 and Notoginsecoside R_1 in Guyuling Tablets by HPLC[J].Journal of Jingmen Vocational Technical College,2009,24(2):43-46. |
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| Authors: | ZHANG Mian |
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| Institution: | Chemical Technology & Medicine College;Jingchu University of Technology;Jingmen;Hubei;448000;China |
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| Abstract: | Objective：To establish the determination method of Ginsenoside Rg1 and Notoginsecoside R1 in Guyuling Tablets. Methods： HPLC with C -18 column was used,and the mobile phase was acetonitrile- water （18：82）. The flow rate was 1.0 mL · min^-1. The detection wavelength was at 203 nm. Results：The standard curve equation of Ginsenoside RgI was Y =2. 829X ＋4. 167 （r =0.999 9 ,n =5） ,the linear range was 3.0 - 22.5 p,g. The standard curve equation of Notoginsecoside R1 was Y = 7. 580X - 3.971 （ r = 0.999 6, n = 5 ）, the linear range was 1.0 - 7.5 ug. The recovery were 98.95% and 99.27% respectively. Conclusion：This method is accurate and sensitive, it can be used for the quality control of Guyuling Tablets. |
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| Keywords: | HPLC |
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