Review of microfluidic microbioreactor technology for high-throughput submerged microbiological cultivation |
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| Authors: | Hanaa M Hegab Ahmed ElMekawy Tim Stakenborg |
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| Institution: | 1.KACST-Intel Consortium Center of Excellence in Nano-Manufacturing Applications (CENA), Riyadh, Saudi Arabia;2.IMEC, Kapeldreef 75, Leuven, Belgium;3.Institute of Advanced Technology and New Materials, City of Scientific Research and Technological Applications, Borg Elarab, Alexandria, Egypt;4.Genetic Engineering and Biotechnology Research Institute, Minufiya University, Sadat City, Egypt |
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| Abstract: | Microbial fermentation process development is pursuing a high production yield. This requires a high throughput screening and optimization of the microbial strains, which is nowadays commonly achieved by applying slow and labor-intensive submerged cultivation in shake flasks or microtiter plates. These methods are also limited towards end-point measurements, low analytical data output, and control over the fermentation process. These drawbacks could be overcome by means of scaled-down microfluidic microbioreactors (μBR) that allow for online control over cultivation data and automation, hence reducing cost and time. This review goes beyond previous work not only by providing a detailed update on the current μBR fabrication techniques but also the operation and control of μBRs is compared to large scale fermentation reactors. |
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