| HPLC法测定众生片中黄芩苷的含量 |
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| 引用本文： | 罗明芳.HPLC法测定众生片中黄芩苷的含量[J].黄冈职业技术学院学报,2007,9(3):89-90. |
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| 作者姓名： | 罗明芳 |
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| 作者单位： | 湖北大学知行学院生工系,湖北,武汉,430010 |
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| 摘 要： | 目的:采用高效液相法测定众生片中黄芩苷的含量。方法:色谱柱为HypersilC18(4.6mm×200mm,5μm;流动相为甲醇-水-磷酸(45:55:0.2);流速为:1ml.min-1;检测波长为278nm.。结果:黄芩苷进样量在0.0150.15ug之间,与峰面积呈良好的线性关系(r=0.9999),平均加样回收率为96.57%,RSD=1.3%(n=5)。结论:本方法操作简单,快捷,可用于测定众生片中黄芩苷的含量。
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| 关 键 词： | HPLC 众生片 黄芩苷 含量测定 |
| 文章编号： | 1672-1047（2007）03-0089-02 |
| 修稿时间： | 2007-07-18 |
Determination of Baicalin in Zhongshengpian by HPLC |
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| Luo Ming-fang.Determination of Baicalin in Zhongshengpian by HPLC[J].Journal of Huanggang Polytechnic,2007,9(3):89-90. |
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| Authors: | Luo Ming-fang |
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| Institution: | Hubei University Zhixing institute biology engineering faculty Wuhan 430010 Hubei |
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| Abstract: | A method was established for determination the content of Baicalin in Zhongshengpian by HPLC.Method: Using RP-HPLC with Hypersil C18 column(200 mm ×4.6 mm,5 μm).The mobile phase consisted of methanol-water-phosphoric acid(45:55:0.2)with 1 mL · min-1 of flow rate,and the detection wavelength was at 278 nm.Results:The Calibration curve was in good linearity over the range of 0.021~0.315ug(r=0.9999),the average recovery rate was 96.57%(RSD=1.30).Conclusion:The determination method is simple and rapid.It can be determine Baicalin in Zhongshengpian. |
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| Keywords: | HPLC Zhongshengpian Baicalin Determination |
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