| ZPI基因外显子-3多态性与妊娠高血压疾病子痫前期的相关性探讨 |
| |
| 引用本文： | 黄斌伦,程屹,王敏,徐秋仙,何云芹.ZPI基因外显子-3多态性与妊娠高血压疾病子痫前期的相关性探讨[J].金华职业技术学院学报,2013(6):70-73. |
| |
| 作者姓名： | 黄斌伦 程屹 王敏 徐秋仙 何云芹 |
| |
| 作者单位： | 金华职业技术学院;金华市中心医院 |
| |
| 基金项目： | 金华市科技局重点资助项目(2009-3-037) |
| |
| 摘 要： | 目的：探讨蛋白z依赖的蛋白酶抑制物（ZPI）基因外显子-3（exon-3）多态性与妊娠高血压疾病子痫前期的相关性。方法：选择健康孕妇30例、妊娠高血压疾病子痫前期36例，用ELISA竞争法测定其血浆ZPI水平。用直接DNA测序法对ZPI基因exon-3测序。结果：ZPI含量健康妊娠组为（144．32±75．45）pg／mL，妊娠高血压疾病子痫前期组为（137．61±42．95）pg／mL，两组之间无显著性差异（F=0．131，P=0．719）。ZPI基因外显子-3测序结果，发现在exon-3137A〉G、181A〉G、481A〉T存在基因多态性，但这三种基因型频率和等位基因频率分布，两组之间均无统计学差异（均P〉0．05）。结论：ZPI基因exon-3137A〉G、181A〉G、481A〉T存在基因多态性，但与妊娠高血压疾病子痫前期关系不大。
|
| 关 键 词： | 妊娠高血压疾病子痫前期 蛋白Z依赖的蛋白酶抑制物 基因多态性 |
Relevant Study of Protein Z-dependent Protease Inhibitor Exon 3 Polymorphisms and the preeclampsia of Hypertensive Disorders in Pregnancy |
| |
| HUANG Bin-lun;CHEN Yi;WANG min;XU Qiu-xian;HE Yun-qin.Relevant Study of Protein Z-dependent Protease Inhibitor Exon 3 Polymorphisms and the preeclampsia of Hypertensive Disorders in Pregnancy[J].Journal of Jinhua College of Profession and Technology,2013(6):70-73. |
| |
| Authors: | HUANG Bin-lun;CHEN Yi;WANG min;XU Qiu-xian;HE Yun-qin |
| |
| Institution: | HUANG Bin-lun;CHEN Yi;WANG min;XU Qiu-xian;HE Yun-qin;Jinhua Polytechnic;Jinhua Municipal Central Hospital; |
| |
| Abstract: | Objective ：To explore the relationship between polymorphisms of protein Z-dependent protease inhibitor （ZPI） exon-3 and the preeclampsia of hypertensive disorders in pregnancy. Methods： The blood samples are collected from 30 cases of healthy pregnant women and 36 with preeclampsia of hypertension disorders in pregnancy. ZPI from blood and protein Z-dependent protease inhibitor（ZPI） exon-3 were detected by enzyme linked immunosorbent assay （ELISA） and direct DNA sequencing. Results： ZPI in the hypertensive disorders in pregnancy group,and healthy women comtrol group were （137.61 ±42.95）pg/ml and （144.32 ±75.45）pg/ml respectively. There were no significant difference between the two groups （F=0.131, P=0.719）. The polymorphisms of ZPI were identified in exon-3 137A〉 G, 181A〉G, 481A〉T, however,there was no significantly association with any of the three genotypic frequency and Alleles frequency distributions （P〉0.05）. Conclusion： There exist gene polymorphisms in ZPI exon-3 137A〉G,181A〉G,481A〉T, but they have little to do with preeclampsia of hypertension disorders in pregnancy. |
| |
| Keywords: | preeclampsia of hypertensive disorders in pregnancy protein Z-dependent proteasinhibitor gene polymorphisms |
| 本文献已被 CNKI 维普 等数据库收录！ |
