| 壳聚糖固定化As1.398中性蛋白酶稳定性的研究 |
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| 引用本文： | 万洪善.壳聚糖固定化As1.398中性蛋白酶稳定性的研究[J].连云港师范高等专科学校学报,2002(2). |
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| 作者姓名： | 万洪善 |
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| 作者单位： | 连云港职业技术学院,化工系,江苏,连云港,222006 |
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| 摘 要： | 研究了以中空球形壳聚糖固定As1 .398中性蛋白酶的方法 ,分析了戊二醛浓度、给酶量对固定化的影响 ,并对固定化酶的性质进行了讨论 ,实验结果显示 ,戊二醛质量浓度为 4 % ,每克载体给酶量 2 5mg时 ,酶活力回收为 65 .4% ,固定化酶的热稳定性、pH稳定性及乙醇的稳定性均高于游离酶
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| 关 键 词： | 球形壳聚糖 戊二醛 固定化 As1.398蛋白酶 |
Study on the Stability of Chitosan-Immobilized As1.398 Neutral Proteinase |
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| WAN Hong-shan.Study on the Stability of Chitosan-Immobilized As1.398 Neutral Proteinase[J].Journal of Lianyungang Teachers College,2002(2). |
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| Authors: | WAN Hong-shan |
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| Abstract: | A method is proposed to immobilize As1.398 neutral proteinase with globular chitosan. Analysis is given to the effects of glutaraldehyde concentration and the ratio of proteinase on the immobilization with discussions on the nature of the immobilized enzyme. Experiment records show that wit 4% of glutaraldehyde and the average ratio of proteinase 25mg per gram,the recycling rate of proteinase can be as high as 65.4%,and the stabilities of the immobilized enzyme,of the pH value and of alcohol are obviously higher than of free enzyme. |
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| Keywords: | globular chitosan immobilize As1 398 neutral proteinase |
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