| HPLC-DAD法测定复方氨酚烷胺片中的咖啡因 |
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| 引用本文： | 杨小萍,谢跃勤,丁茹.HPLC-DAD法测定复方氨酚烷胺片中的咖啡因[J].安徽教育学院学报,2011,29(3):77-79. |
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| 作者姓名： | 杨小萍 谢跃勤 丁茹 |
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| 作者单位： | 合肥师范学院,化学化工系,安徽,合肥,230061 |
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| 摘 要： | 建立了高效液相色谱（HPLC）测定复方氨酚烷胺片中咖啡因的方法。在流动相为甲醇-水（体积比为70：30）、检测波长为270 nm、流速为1.0mL/min、柱温为30℃、进样量为20μL的色谱条件下对药品中的咖啡因含量进行测定。实验测得咖啡因浓度在12~240μg/mL范围内,浓度与峰面积呈良好的线性关系（r=0.9999）,加标回收率为100.61%,相对标准偏差RSD小于1%。用该方法测定复方氨酚烷胺片中的咖啡因,操作简单、快速、准确,结果令人满意。
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| 关 键 词： | HPLC 咖啡因 复方氨酚烷胺片 |
HPLC Determination of "Exclamation Mark" Brand Compound Paracetamol and Amantadine Hydrochloride Tablets Caffeine |
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| YANG Xiao-ping,XIE Yue-qin,DING Ru.HPLC Determination of "Exclamation Mark" Brand Compound Paracetamol and Amantadine Hydrochloride Tablets Caffeine[J].Journal of Anhui Institute of Education,2011,29(3):77-79. |
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| Authors: | YANG Xiao-ping XIE Yue-qin DING Ru |
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| Institution: | (Department of Chemistry and Chemical Engineering,Hefei Normal University,Hefei 230061,China) |
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| Abstract: | This study established an accurate determination of ＂Exclamation Mark＂ brand compound paracetamol and amantadine hydrochloride tablets by high performance liquid chromatographic determination of caffeine method,that is,the mobile phase was methanol-water（70：30）,detection wavelength 270 nm,flow rate 1.0mL/min,column temperature was 30 ℃,the injection volume was 20uL drugs under the chromatographic conditions were determined in caffeine.Experimentally measured range of caffeine concentration in the 12-240ug/mL,concentration and peak area was a good linear relationship（r=0.9999）,recovery was 100.61%,RSD relative standard deviation less than 1%.This method permitted paracetamol and amantadine hydrochloride tablets compound caffeine,simple,rapid,accurate and satisfactory results. |
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| Keywords: | HPLC |
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