| 一种简便灵敏检测半胱氨酸的共振散射光谱法 |
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| 引用本文： | 许丽莉,陈华舟,黄娅平,陈连泽.一种简便灵敏检测半胱氨酸的共振散射光谱法[J].钦州学院学报,2014(2):4-8. |
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| 作者姓名： | 许丽莉 陈华舟 黄娅平 陈连泽 |
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| 作者单位： | [1]钦州学院海洋学院,广西钦州535000 [2]桂林理工大学理学院,广西桂林541000 |
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| 基金项目： | 钦州学院校级青年基金项目：氧化石墨烯的制备及其生物大分子的相互作用研究（2013XJKY-49QA）. |
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| 摘 要： | 采用硼氢化钠还原法制备10nm的纳米银。纳米银在NaCl作用下会发生聚集，在469nm产生一个较强共振散射峰，在pH值为6．8的Na2HPO4-NaH2PO4缓冲液中，半胱氨酸（Cys）吸附在纳米银表面，使纳米银聚集程度减弱，导致体系在469nm的共振散射峰减弱。在选定条件下，Cys浓度在5-300nmol／L范围内与469nm的共振散射光强度减弱值△I469nm呈线性关系，回归方程为△I469=11．55C＋387．92，相关系数和方法检出限分别为0．998、0．5nM。该法可用于草莓汁添加剂半胱氨酸的含量的检测，结果满意。
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| 关 键 词： | 半胱氨酸 纳米银 共振散射光谱 |
A Simple And Sensitive Detection of Cysteine Using Resonance Scattering Spectroscopy |
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| Institution: | XU Li-li, CHEN Hua-zhou, HUANG Ya-ping, CHEN Lian-zhe ( 1. College of Marine, Qinzhou University, Qinzhou 535000, China ; College of Science, Guilin Technology University, Guilin 541000, China) |
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| Abstract: | Nanosilve in size of 10 nm was prepared by the NaBH4 procedure. In NaCl solution, the nanosilver were aggregated to form big particles that exhibited a strong resonance Rayleigh scattering （RRS） peak at 469nm. In pH6.8 Na2HPO4 - NaH2PO4 acid buffer solution, Cysteine （Cys） adsorbed on the surface of nanosilver, so that the degree ofaggregationof nanosilver weakened which led to the resonance scattering （RS） peak at 469 nm declined greatly. Under the selected conditions, the reduced RS intensity （ AI469 nm ） is linear to Cys concentration in the range of 5-300 nmol/L, with a regression equation of AI469 = 11.55C＋387.92 ,coefficient of 0.998, and a detection limit of 0.5 nmol/L. This nanogold RS method was applied to determination of Cys in waste water samples, with satisfactory results. |
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| Keywords: | Cysteine nanosilver resonance scattering spectroscopy |
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