| 大肠杆菌生产基因重组草鱼生长激素的分离纯化 |
| |
| 引用本文： | 张为民,林浩然,沈文英,马广智.大肠杆菌生产基因重组草鱼生长激素的分离纯化[J].中山大学学报论丛,1995(1). |
| |
| 作者姓名： | 张为民 林浩然 沈文英 马广智 |
| |
| 作者单位： | 中山大学生物学系!广州510275(张为民，林浩然，沈文英)，华南师范大学生物学系(马广智) |
| |
| 摘 要： | 草鱼生长激素cDNA在大肠杆菌细胞中表达后，重组草鱼生长激素在胞内形成不溶性的包含体．这种包含体在洗涤、变性、复性和C12反相层析等步骤后，产物再经快速蛋白液相色谱，重组草鱼生长激素在SDS-聚丙烯酰凝胶电泳中显示均一性，纯度可达90％放射免疫分析表明：重组草鱼生长激素具有草鱼生长激素的免疫活住．分离纯化产率约为1L细菌培养液1～2m重组草鱼生长激素．
|
| 关 键 词： | 大肠杆菌 包含体 基因重组草鱼生长激素 |
Isolation and Purification of Recombinant Grass Carp Growth Hormone Produced in Escherichia coli |
| |
| Zhang Weimin, Lin Haoren, Shen Wenyin, Ma Guangzhi.Isolation and Purification of Recombinant Grass Carp Growth Hormone Produced in Escherichia coli[J].Supplement to the Journal of Sun Yatsen University,1995(1). |
| |
| Authors: | Zhang Weimin Lin Haoren Shen Wenyin Ma Guangzhi |
| |
| Abstract: | Cloned cDNA of grass carn growth hormone (rgGH) was expressed in Escherichia coli cells and the rgGH was accumulated as insoluble inclusion theies. The inclusion bodies were washed and denatured. After renaturation the rgGH was purified by C15 revcrse phase chromatography and fast protein liquid chromatography (FPLC) to 90% purity. The results of radioimmunoassay show that the rgGH possesses immunoreatctivity of growth hormone. The final yield of the rgGH was 1 ~ 2 mg per liter of bacteria culture. |
| |
| Keywords: | Escherichia coli inclusion body recombinant grass carp growth hormone |
| 本文献已被 CNKI 等数据库收录！ |
