| 贵州铜仁产广谱抑菌作用细菌素乳酸菌的筛选及鉴定 |
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| 引用本文： | 胡美忠,张新卓,刘芸.贵州铜仁产广谱抑菌作用细菌素乳酸菌的筛选及鉴定[J].铜仁学院学报,2014(4):32-36. |
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| 作者姓名： | 胡美忠 张新卓 刘芸 |
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| 作者单位： | 铜仁职业技术学院,贵州铜仁550025 |
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| 摘 要： | 从贵州铜仁产发酵食品中分离纯化出70余株乳酸菌，采用Agar-spot-test初筛与排除酸、过氧化氢抑制后复筛出一株能产广谱抑菌作用细菌素的乳酸菌（编号G55），经生理生化及16S rDNA鉴定可知G55为植物乳杆菌。抑菌谱实验表明，G55产生的细菌素能抑制革兰阳性菌及革兰阴性菌的生长；蛋白酶实验表明，G55产生的细菌素对胃蛋白酶、蛋白酶K敏感，对胰蛋白酶、α凝乳蛋白酶部分敏感。
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| 关 键 词： | 植物乳杆菌 广谱抑菌 细菌素 |
On the Sift and Identification of the Broad-spectrum Antibacterial Bacteriocin Produced in Tongren,Guizhou |
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| HU Mei-zhong,ZHANG Xin-zhuo,Liu Yun.On the Sift and Identification of the Broad-spectrum Antibacterial Bacteriocin Produced in Tongren,Guizhou[J].Journal of Tongren University,2014(4):32-36. |
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| Authors: | HU Mei-zhong ZHANG Xin-zhuo Liu Yun |
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| Institution: | ( Tongren Polytechnic College, Tongren, Guizhou 550025, China ) |
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| Abstract: | More than 70 strains of lactic acid bacteria are separated and purified from the fermented foods made in Tongren, Guizhou. First, they are preliminarily sifted by means of agar-spot-test and then excluded from the inhibition of the acid and hydrogen peroxide. After a second sift, a strain of lactic acid bacteria named G55 which can produce broad-spectrum antibacterial bacteriocin is chosen from them. After the physiobiochemical experiment and the 16SrDNA identification, it is concluded that G55 is an actobacillus plantarum. According to the antibacterial spectrum experiment, it shows that the bacteriocin produced by G55 can inhibit the growth of both gram-positive bacteria and gram-negative bacteria. Meanwhile, the experiment of protease shows that the bacteriorin is sensitive to pepsin and proteinase K and is partially sensitive to trypsin andαcurd protease. |
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| Keywords: | lactobacillus plantarum broad-spectrum bacteriostasis bacteriocins |
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