| 模拟3500m不同低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响 |
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| 引用本文： | 李洁,邢良美.模拟3500m不同低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响[J].上海体育学院学报,2012(1):51-55. |
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| 作者姓名： | 李洁 邢良美 |
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| 作者单位： | 西北师范大学体育学院 |
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| 基金项目： | 国家自然科学基金资助项目(31060145) |
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| 摘 要： | 目的:探讨4种低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响。方法:将雄性Wistar大鼠随机均分为5组,即低住低训组(LoLo)、高住高练组(HiHi)、高住低训组(HiLo)、低住高练组(LoHi)及高住高练低训组(HiHiLo)。以当地环境为基点作为常氧环境,采用低压氧舱模拟低氧环境。依实验方案,各组大鼠分别在常氧或/和低氧环境中居住及递增负荷训练5周,每周训练6 d。各组大鼠在最后1次训练后,在常氧环境中恢复3 d,力竭运动后即刻取心肌样本。用差速离心法提取心肌线粒体,分别测定丙二醛含量、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶(catalase,CAT)活性及呼吸链酶复合体I～IV(CI～IV)活性。结果:与LoLo组相比,心肌组织中HiHi组GSH-Px及CAT活性均显著升高,HiLo组CAT活性显著升高,LoHi和HiHiLo组SOD、GSH-Px及CAT活性均显著升高,4种低氧训练模式MDA含量均显著降低。与LoLo组相比,HiHiLo组心肌线粒体SOD活性显著升高,HiHi、HiLo和HiHiLo组MDA含量均显著降低。与LoLo组相比,HiHi组心肌线粒体CIV活性显著升高,HiHiLo组CI、CII和CIV活性均显著提高,HiHi、LoHi及HiHiLo组CIII活性均显著降低。结论:HiHiLo可能是较好的低氧训练模式。
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| 关 键 词： | 低氧训练 大鼠 心肌 线粒体 抗氧化酶 丙二醛 呼吸链 酶复合体 |
The Effects of Simulated 3 500 m Different Hypoxic Training on Free Radical Metabolism and Respiratory Chain Function of Mitochondrial in Myocardium after Exhaustive Running in Rat |
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| LI Jie,XING Liang-mei.The Effects of Simulated 3 500 m Different Hypoxic Training on Free Radical Metabolism and Respiratory Chain Function of Mitochondrial in Myocardium after Exhaustive Running in Rat[J].Journal of Shanghai Physical Education Institute,2012(1):51-55. |
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| Authors: | LI Jie XING Liang-mei |
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| Institution: | College of Physical Education,Northwest Normal University,Lanzhou 730070,Gansu,China |
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| Abstract: | This paper studies the effects of hypoxic training on mitochondrial free radical metabolism and activities of respiratory chain complexes in mitochondria of myocardium in rats.Forty healthy male Wistar rats were randomized to 5 groups: living low-training low(LoLo),living high-training high(HiHi),living high-training low(HiLo),living low-training high(LoHi),and living high-exercise high-training low(HiHilo).The local environment takes normoxic environment.A hypoxic chamber was employed to simulate hypoxic environment.All the animals were subjected to 5-week living and training in normoxic or/an hypoxic environment.Before exhaustive running,the animals stayed in normoxic for 3 days,myocardium was prepared immediately after enhaustive running.Myocardium mitochondria were extracted by different centrifugation.Spectra-photometric analysis was used to evaluate activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),catalase(CAT),malondialdehyde(MDA) level and respiratory chain complex I-IV activities in myocardium homogenate and mitochondria.Results showed that GSH-Px and CAT activities of myocardium homogenate in HiHi groups were significantly increased,CAT activity in HiLo group was significantly increased.MDA level in 4 hypoxic training modes were significantly decreased compared with those in LoLo group.Myocardium mitochondrial activities of SOD was remarkably higher in HiHiLo,MDA level in HiHi,HiLo and HiHiLo group was significantly lower than those in LoLo groups.Meanwhile,CIV activity in HiHi groups was increased significantly,and CI,CII and CIV activates in HiHiLo group was increased remarkably and CIII activity in HiHi,LoHi,HiHiLo groups was decreased remarkably compared with those in LoLo group.These results suggest that HiHiLo might be an ideal hypoxic training mode. |
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| Keywords: | hypoxic training rat myocardium tissue mitochondria antioxidant malondialdehyde respiratory chain multi-enzymatic complexes |
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