| 分子排阻色谱法测定云芝糖肽的分子量分布 |
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| 引用本文： | 高平.分子排阻色谱法测定云芝糖肽的分子量分布[J].泰州职业技术学院学报,2011,11(2):61-62,34. |
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| 作者姓名： | 高平 |
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| 作者单位： | 江苏江山制药有限公司,江苏靖江,214500 |
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| 摘 要： | 目的建立分子排阻色谱法测定云芝糖肽的分子量分布的方法。方法用分子排阻色谱法,色谱柱：TSKgelG 4000SW（7.5mm×300m m）柱,流动相：0.02mol/L醋酸钠溶液,柱温：30℃,已知分子量右旋糖酐分子量标准品作校正标准,示差折光检测器。流速：0.50m l/min;进样量：10μL。结果根据建立的方法测定,分析了3批样品,得到云芝糖肽的排阻色谱图并计算出重均分子量大于40000的相对百分比为59.7%,63.6%,66.6%。结论该方法简便、快速,可用云芝糖肽的质量控制。
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| 关 键 词： | 云芝糖肽 分子排阻色谱法 分子量分布 测定 |
Determination of the Molecular Weight Distribution of Posaverptidum(PSP) by GPC |
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| GAO Ping.Determination of the Molecular Weight Distribution of Posaverptidum(PSP) by GPC[J].Journal of Taizhou Polytechnical Institute,2011,11(2):61-62,34. |
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| Authors: | GAO Ping |
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| Institution: | GAO Ping(Aland(Jiangsu) Nutraceutical Co.,Ltd.,Jingjiang Jiangsu 214500,China) |
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| Abstract: | Objective To establish GPC method of analysis the molecular weight and molecular weight distribution of Posaverptidum.Methods Using HPSEC method,the TSK G4000SW column was used at 30℃,with 0.02 mol/L sodium acetate solution were used as mobile phase,and with a flow rate of 0.50 ml/min and an injection volume of 10 L,using dextrans with known molecular weights as calibration standards,and RI detector was used.Results A method was established and three samples were analyzed by the method.The chromatograms and Weight-average molecular weights and Relative content of weight-average molecular weight more than 40,000 of polysaccharide in the samples were obtained 59.7%,63.6%,66.6%.Conclusion The method is simple,rapid and convenient.It can be used in the quality control of Posaverptidum. |
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| Keywords: | posaverptidum GPC molecular weight distribution determination |
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