Induction of IgE antibody response by the green seed extract of<Emphasis Type="Italic">Vigna sinensis</Emphasis> in mice |
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| Authors: | T Raghava Rao D N Rao B Veerendra Kumar P Aparanji K Srinivas Rao R Athota |
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| Institution: | (1) Department of Biochemistry, AIIMS, 110029 New Delhi;(2) Department of Biochemistry, Andhra University, 530 003 Visakhapatnam, Andhra Pradesh |
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| Abstract: | Sensitization to ingested foods is a known fact and several food allergens have been characterized. It has been observed in
our survey that the people complained of allergic symptoms after consumption of the vegetableVigna sinensis. In this study, the experiments were carried to investigate the IgE antibody response against the green seed extract of vigna
sinensis in mice and found that the primary, secondary and tertiary immunization with or without adjuvant by different doses
induced a significant production of IgE antibodies. The presence of IgE antibodies in the mice sera were determined by passive
cutaneous anaphylaxis and enzyme linked immunosorbent assay. It was also confirmed that these allergens were found to be heat
resistant and shared a common epitope(s) with the other legume foods, as evidenced by the cross-reactive studies. |
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| Keywords: | Vigna sinensis Food allergy PCA ELISA |
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