| 雷氏大疣蛛毒素-V的分离纯化与初步活性鉴定 |
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| 引用本文： | 曾雄智,梁宋平.雷氏大疣蛛毒素-V的分离纯化与初步活性鉴定[J].怀化学院学报,2007(4). |
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| 作者姓名： | 曾雄智 梁宋平 |
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| 作者单位： | 湖南师范大学生命科学学院 湖南长沙410081 |
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| 基金项目： | 国家自然科学基金重点项目(NO30430170)，国家863专题2006AA02Z141，湖南省教育厅基金项目(06C503) |
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| 摘 要： | 雷氏大疣蛛(Macrothele raveni)是最近发现的异仿蛛科大疣蛛属的蜘蛛新种.雷氏大疣蛛毒素-V就是以该蜘蛛粗毒为原料,利用阴、阳离子交换层析和反相高效液相色谱分离纯化得到并命名的一种新型蜘蛛毒素.利用基质辅助激光解析电离飞行时间质谱仪分析得知它的相对分子质量为3133.48;通过初步活性研究证明雷氏大疣蛛毒素-V是一种昆虫专一性神经毒素.
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| 关 键 词： | 蜘蛛 雷氏大疣蛛毒素-V 离子交换层析 反相高效液相色谱 基质辅助激光解析电离飞行时间质谱 |
Purification and Preliminary Active Characterization of Raventoxin-V,a Novel Insecticidal Toxin Isolated from the Spider Macrothele Rraveni |
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| ZENG Xiong-zhi,LIANG Song-ping.Purification and Preliminary Active Characterization of Raventoxin-V,a Novel Insecticidal Toxin Isolated from the Spider Macrothele Rraveni[J].Journal of Huaihua University,2007(4). |
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| Authors: | ZENG Xiong-zhi LIANG Song-ping |
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| Abstract: | The spider Macrothele raveni was recently identified as a new species of Genus Macrothele.A novel neurotoxic peptide named Raventoxin-V was purified from the venom of the spider Macrothele raveni by cation-exchange,anion-exchange and reverse phase high performance liquid chromatography.The relative molecular mass of the toxin was determined to be 3133.48 by matrix assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS).According to the preliminary activity research,it has been proved that Raventoxin-V is a novel insectticidal neurotoxin. |
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| Keywords: | spider raventoxin-V ion-exchange chromatography reverse-phase high performance liquid chromatography MALDI-TOF MS |
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