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Mutagenic effects of chromium trioxide on root tip cells of <Emphasis Type="Italic">Vicia faba</Emphasis>
Authors:Email author" target="_blank">Qian?Xiao-wei?Email author
Institution:(1) School of Life and Environmental Science, Wenzhou Normal College, 325003 Wenzhou, China
Abstract:In this study on the mutagenic effects of different concentrations of chromium trioxide (CrO3) on Vicia faba root tip, micronucleus assay and chromosome aberration assay were used to determine the mitotic indexes, micronucleus rate and chromosome aberration rate of Vicia faba root tip cells. The results showed that the effects of CrO3 concentration on the mitotic indexes were complicated. CrO3 increases the micronucleus rate of Vicia faba root tip cells. It was found that within certain range of CrO3 concentration the micronucleus rate increased systematically with increased concentration of CrO3, but that the micronucleus rate decreased at higher level of CrO3 and that CrO3 also caused various types of chromosome aberration at a rate which increased systematically with increased concentration of CrO3. We concluded that CrO3 has significant mutagenic effect on Vicia faba root tip cells. Project supported by Wenzhou Technology Bureau (No. S2002A015) and Wenzhou “551” Talented People Fund of China
Keywords:Chromium trioxide (CrO3)            Vicia faba            Mitotic index  Micronucleus rate  Rate of chromosome aberration
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